Search Results - "Merkle, T."

Modeling Human Disease with Pluripotent Stem Cells: from Genome Association to Function by Merkle, Florian T., Eggan, Kevin

“…Mechanistic insights into human disease may enable the development of treatments that are effective in broad patient populations. The confluence of…”
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Mosaic Organization of Neural Stem Cells in the Adult Brain by Merkle, Florian T, Mirzadeh, Zaman, Alvarez-Buylla, Arturo

“…The in vivo potential of neural stem cells in the postnatal mouse brain is not known, but because they produce many different types of neurons, they must be…”
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Population-scale single-cell RNA-seq profiling across dopaminergic neuron differentiation by Jerber, Julie, Seaton, Daniel D., Cuomo, Anna S. E., Kumasaka, Natsuhiko, Haldane, James, Steer, Juliette, Patel, Minal, Pearce, Daniel, Andersson, Malin, Bonder, Marc Jan, Mountjoy, Ed, Ghoussaini, Maya, Lancaster, Madeline A., Marioni, John C., Merkle, Florian T., Gaffney, Daniel J., Stegle, Oliver

“…Studying the function of common genetic variants in primary human tissues and during development is challenging. To address this, we use an efficient…”
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Adult neural stem cells in distinct microdomains generate previously unknown interneuron types by Merkle, Florian T, Fuentealba, Luis C, Sanders, Timothy A, Magno, Lorenza, Kessaris, Nicoletta, Alvarez-Buylla, Arturo

“…The authors report the generation of four previously unknown olfactory bulb interneuron subtypes by adult neural stem cells, organized into surprisingly small…”
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Directed Differentiation and Functional Maturation of Cortical Interneurons from Human Embryonic Stem Cells by Maroof, Asif M., Keros, Sotirios, Tyson, Jennifer A., Ying, Shui-Wang, Ganat, Yosif M., Merkle, Florian T., Liu, Becky, Goulburn, Adam, Stanley, Edouard G., Elefanty, Andrew G., Widmer, Hans Ruedi, Eggan, Kevin, Goldstein, Peter A., Anderson, Stewart A., Studer, Lorenz

“…Human pluripotent stem cells are a powerful tool for modeling brain development and disease. The human cortex is composed of two major neuronal populations:…”
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Origin and function of olfactory bulb interneuron diversity by Lledo, Pierre-Marie, Merkle, Florian T, Alvarez-Buylla, Arturo

“…In adult rodents, subventricular zone (SVZ) astrocytes (B cells) function as primary progenitors in the generation of new neurons that migrate to the olfactory…”
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Efficient CRISPR-Cas9-Mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus by Merkle, Florian T., Neuhausser, Werner M., Santos, David, Valen, Eivind, Gagnon, James A., Maas, Kristi, Sandoe, Jackson, Schier, Alexander F., Eggan, Kevin

“…The CRISPR-Cas9 system has the potential to revolutionize genome editing in human pluripotent stem cells (hPSCs), but its advantages and pitfalls are still…”
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Generation of neuropeptidergic hypothalamic neurons from human pluripotent stem cells by Merkle, Florian T, Maroof, Asif, Wataya, Takafumi, Sasai, Yoshiki, Studer, Lorenz, Eggan, Kevin, Schier, Alexander F

“…Hypothalamic neurons orchestrate many essential physiological and behavioral processes via secreted neuropeptides, and are relevant to human diseases such as…”
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Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1 by Kiskinis, Evangelos, Sandoe, Jackson, Williams, Luis A., Boulting, Gabriella L., Moccia, Rob, Wainger, Brian J., Han, Steve, Peng, Theodore, Thams, Sebastian, Mikkilineni, Shravani, Mellin, Cassidy, Merkle, Florian T., Davis-Dusenbery, Brandi N., Ziller, Michael, Oakley, Derek, Ichida, Justin, Di Costanzo, Stefania, Atwater, Nick, Maeder, Morgan L., Goodwin, Mathew J., Nemesh, James, Handsaker, Robert E., Paull, Daniel, Noggle, Scott, McCarroll, Steven A., Joung, J. Keith, Woolf, Clifford J., Brown, Robert H., Eggan, Kevin

“…Although many distinct mutations in a variety of genes are known to cause Amyotrophic Lateral Sclerosis (ALS), it remains poorly understood how they…”
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Radial Glia Give Rise to Adult Neural Stem Cells in the Subventricular Zone by Merkle, Florian T., Tramontin, Anthony D., García-Verdugo, José Manuel, Alvarez-Buylla, Arturo, Nottebohm, Fernando

“…Neural stem cells with the characteristics of astrocytes persist in the subventricular zone (SVZ) of the juvenile and adult brain. These cells generate large…”
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Adult ependymal cells are postmitotic and are derived from radial glial cells during embryogenesis by Spassky, Nathalie, Merkle, Florian T, Flames, Nuria, Tramontin, Anthony D, García-Verdugo, José Manuel, Alvarez-Buylla, Arturo

“…Ependymal cells on the walls of brain ventricles play essential roles in the transport of CSF and in brain homeostasis. It has been suggested that ependymal…”
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The genetic architecture of DNA replication timing in human pluripotent stem cells by Ding, Qiliang, Edwards, Matthew M., Wang, Ning, Zhu, Xiang, Bracci, Alexa N., Hulke, Michelle L., Hu, Ya, Tong, Yao, Hsiao, Joyce, Charvet, Christine J., Ghosh, Sulagna, Handsaker, Robert E., Eggan, Kevin, Merkle, Florian T., Gerhardt, Jeannine, Egli, Dieter, Clark, Andrew G., Koren, Amnon

“…DNA replication follows a strict spatiotemporal program that intersects with chromatin structure but has a poorly understood genetic basis. To systematically…”
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Ultrasound-guided localization of the radial nerve along the humerus: providing reference points for safer upper arm surgery by Da Silva, T, Mueck, D, Knop, C, Merkle, T

“…The close proximity of the radial nerve to the humerus poses a risk during upper arm surgery. Although the general course of the radial nerve is well-known,…”
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Distinctive Neurons of the Anterior Cingulate and Frontoinsular Cortex: A Historical Perspective by Seeley, William W., Merkle, Florian T., Gaus, Stephanie E., Craig, A.D. (Bud), Allman, John M., Hof, Patrick R.

“…Human anterior cingulate and frontoinsular cortices participate in healthy social-emotional processing. These regions feature 2 related layer 5 neuronal…”
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Evolutionarily conserved regulation of hypocretin neuron specification by Lhx9 by Liu, Justin, Merkle, Florian T, Gandhi, Avni V, Gagnon, James A, Woods, Ian G, Chiu, Cindy N, Shimogori, Tomomi, Schier, Alexander F, Prober, David A

“…Loss of neurons that express the neuropeptide hypocretin (Hcrt) has been implicated in narcolepsy, a debilitating disorder characterized by excessive daytime…”
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The mouse C9ORF72 ortholog is enriched in neurons known to degenerate in ALS and FTD by Suzuki, Naoki, Maroof, Asif M, Merkle, Florian T, Koszka, Kathryn, Intoh, Atsushi, Armstrong, Ian, Moccia, Rob, Davis-Dusenbery, Brandi N, Eggan, Kevin

“…In this study, the authors show that the mouse ortholog of the amyotropic lateral sclerosis/frontotemporal dementia (ALS/FTD)-associated human locus C9ORF72…”
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A Short Sequence Targets Transmembrane Proteins to Primary Cilia by Macarelli, Viviana, Harding, Edward C, Gershlick, David C, Merkle, Florian T

“…Primary cilia are finger-like sensory organelles that extend from the bodies of most cell types and have a distinct lipid and protein composition from the…”
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Generation and Characterization of Functional Human Hypothalamic Neurons by Kirwan, Peter, Jura, Magdalena, Merkle, Florian T

“…Neurons in the hypothalamus orchestrate homeostatic physiological processes and behaviors essential for life. Defects in the function of hypothalamic neurons…”
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The dual aptamer approach: rational design of a high-affinity FAD aptamer by Merkle, T, Holder, I T, Hartig, J S

“…A design strategy for high-affinity aptamers of complex biomolecules is presented. We developed an RNA with FAD-binding properties by combining known ATP- and…”
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Proopiomelanocortin Processing in the Hypothalamus Is Directly Regulated by Saturated Fat: Implications for the Development of Obesity by Razolli, Daniela S, de Araújo, Thiago M, Sant Apos Ana, Marcella R, Kirwan, Peter, Cintra, Dennys E, Merkle, Florian T, Velloso, Licio A

“…In outbred mice, susceptibility or resistance to diet-induced obesity is associated with rapid changes in hypothalamic proopiomelanocortin (POMC) levels. Here,…”
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