Molecular Evidence for Divergent Breeding Groups of Walleye ( Stizostedion vitreum) in Tributaries to Western Lake Erie

Molecular evidence from mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) supports the existence of genetically different spawning groups of walleye (Stizostedion vitreum) among the Maumee, Sandusky, and Grand rivers that flow into Lake Erie. Three separate regions of the m...

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Bibliographic Details
Published in:Journal of Great Lakes research Vol. 22; no. 2; pp. 280 - 288
Main Authors: Merker, R.J., Woodruff, R.C.
Format: Journal Article
Language:English
Published: Elsevier B.V 1996
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Summary:Molecular evidence from mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) supports the existence of genetically different spawning groups of walleye (Stizostedion vitreum) among the Maumee, Sandusky, and Grand rivers that flow into Lake Erie. Three separate regions of the mtDNA molecule (the D-loop region, the NADH dehydrogenase 3, 4, and 4L genes, and the 12S and 16S rRNA genes) from female walleye eggs were amplified using the polymerase chain reaction technique. Amplifications were digested with nine different four base cutting restriction endonucleases and were resolved on 9% polyacrylamide gels. The D-loop and the NADH genes produced RFLPs, revealing a total of eight different haplotypes. The frequencies of haplotypes within the Maumee River spawning walleye were significantly different from the haplotypes within the Sandusky River. Frequencies of the haplotypes within the Grand River spawning walleye were not statistically different from those of the Maumee or Sandusky rivers. These results support the hypothesis that the Maumee and Sandusky River spawning walleye are members of two different breeding groups. The molecular identification of these walleye breeding groups may help in future fisheries management decisions.
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ISSN:0380-1330
DOI:10.1016/S0380-1330(96)70955-3