In Vitro and In Vivo Cytogenotoxic Effects of Hot Aqueous Extract of Achyrocline satureioides (Lam.) DC.

In this work we extend the toxicological studies of hot aqueous extract of A. satureioides (As-HAE) evaluating cytotoxic and apoptotic effects on human peripheral blood mononuclear cells (PBMCs). We also determine genotoxic action of this extract in vivo. In addition, the extract was chemically char...

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Published in:	BioMed research international Vol. 2015; no. 2015; pp. 1 - 12
Main Authors:	Comini, L. R., Sabini, L., Zanon, S. M., Reinoso, E. B., Merckis, C., Montironi, I., Bacchetti, R., Escobar, F., Sabini, C., Cariddi, L. N., Núñez Montoya, S.
Format:	Journal Article
Language:	English
Published:	Cairo, Egypt Hindawi Publishing Corporation 01-01-2015 John Wiley & Sons, Inc Hindawi Limited
Subjects:	Achyrocline - chemistry Animals Apoptosis - drug effects Bioflavonoids Bone marrow Cold Cytotoxicity DNA Damage - drug effects DNA Fragmentation - drug effects Flavones Flavonoids Flavonoids - chemistry Flavonoids - pharmacology Free radicals Genetic toxicology Health aspects Humans Leukocytes, Mononuclear - drug effects Luteolin - isolation & purification Luteolin - pharmacology Materia medica, Vegetable Medical research Medicine, Experimental Mice Plant extracts Plant Extracts - chemistry Plant Extracts - pharmacology Quercetin - analogs & derivatives Quercetin - isolation & purification Quercetin - pharmacology Studies Europe
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Summary:	In this work we extend the toxicological studies of hot aqueous extract of A. satureioides (As-HAE) evaluating cytotoxic and apoptotic effects on human peripheral blood mononuclear cells (PBMCs). We also determine genotoxic action of this extract in vivo. In addition, the extract was chemically characterized. Finally, we established a comparison with previous data of cold aqueous extract. The As-HAE induced cytotoxicity on PBMCs determined by trypan blue dye exclusion (CC50 = 653 μg/mL) and MTT (CC50 = 588 μg/mL) assays being more toxic than cold extract. However, As-HAE as well as cold extract did not induce apoptosis measured by Hoechst 33258 staining, TUNEL assay, and DNA fragmentation analysis. The in vivo micronucleus test showed that As-HAE exerted cytogenotoxic effects on bone marrow of mice, contrary to what was observed with cold extract. The chemical study of As-HAE allowed identifying the flavonoids found in cold extract: luteolin, quercetin, and 3-O-methylquercetin, but at higher concentrations. We suggest that toxic effects induced by As-HAE could be due to high concentrations of these flavonoids. Given that As-HAE is the most used in folkloric medicine, its administration should be controlled in order to prevent potential cell damage.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Academic Editor: Isabel C. F. R. Ferreira
ISSN:	2314-6133 2314-6141
DOI:	10.1155/2015/270973