An in Vitro Effectiveness Evaluation of Chemical Agents for Toothbrushes Disinfection

An in Vitro Effectiveness Evaluation of Chemical Agents for Toothbrushes Disinfection

Abstract Objective: To evaluate in the vitro effectiveness of three chemical agents for toothbrush disinfection. Material and Methods: Sixteen new toothbrushes were evaluated, previously sterilized and classified in five experimental groups (n=3) and one item as control. Three chemical agents were a...

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Bibliographic Details
Published in:Pesquisa brasileira em odontopediatria e clínica integrada Vol. 19; no. 1; pp. 1 - 8
Main Authors: Merchán, Ingrid, Merino-Alado, Rommie Lucia, Briceño, Elsi Natali, Moronta, Gustavo, Oviedo, Maria Jose, Ortega, Anny, Perez, Ericka, Pestana, Andrea, Rodriguez, Maria Alejandra
Format: Journal Article
Language:English
Published: Associação de Apoio à Pesquisa em Saúde Bucal 2019
Subjects:
DENTISTRY, ORAL SURGERY & MEDICINE
Toothbrushing
Disinfection
Microbiology
Sodium Hypochlorite
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Summary:Abstract Objective: To evaluate in the vitro effectiveness of three chemical agents for toothbrush disinfection. Material and Methods: Sixteen new toothbrushes were evaluated, previously sterilized and classified in five experimental groups (n=3) and one item as control. Three chemical agents were assessed: 0.12% Chlorhexidine gluconate (CHX), essential oil mouth rinse (Listerine) and 3.5% Sodium hypochlorite (NaOCl). The five selected strains were inoculated on toothbrushes and incubated for a 24 hours period and 37°C temperature in aerobic conditions. The incubated toothbrushes were immersed for a 15 min period into selected chemical agents and after drying in a controlled air stream, again re-cultured into enriched broth. A comparison was made between the initial and final microorganisms density recovered after chemical disinfection based on Mc Farland scale. The data obtained was compared by descriptive analysis and ANOVA methodology. Results: 3.5% NaOCl was the most effective chemical agent for toothbrush disinfection followed by CHX; Listerine was not effective to eliminate the inoculated bacteria in toothbrushes. Conclusion: 3.5% NaOCl and 0.12% CHX are the most effective chemical agents for toothbrush disinfection and Listerine was only effective against C. albicans.
ISSN:1519-0501
1983-4632
DOI:10.4034/PBOCI.2019.191.08