Cholesterol effect on Apolipoprotein B secretion and binding of low-density lipoproteins hepatoma HepG2 Cells

Cholesterol effect on Apolipoprotein B secretion and binding of low-density lipoproteins hepatoma HepG2 Cells

The effect of cholesterol, oleic acid and recombinant complexes containing apolipoprotein Al/dimyristoylphosphatidylcholine (apoAl/DMPC) on apolipoprotein B (apoB) secretion and uptake of 125I-labeled low-density lipoproteins (LDL) by cultured human hepatoma HepG2 cells was studied. Addition of diff...

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Bibliographic Details
Published in:Biopolimery i kletka Vol. 5; no. 2; pp. 74 - 78
Main Authors: Fuki, I. V., Preobrazhensky, S. N., Misharin, A. Yu, Bushmakina, N. G., Menshchikov, G. B., Repin, V. S.
Format: Journal Article
Language:English
Published: Kiev Natsional'na Akademiya Nauk Ukrainy - National Academy of Sciences of Ukraine 20-03-1989
Subjects:
Apolipoprotein B
Apolipoproteins
Cell culture
Cholesterol
Dimyristoylphosphatidylcholine
Ethanol
Hepatoma
Lipoproteins
Liver cancer
Low density lipoprotein
Oleic acid
Secretion
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Summary:The effect of cholesterol, oleic acid and recombinant complexes containing apolipoprotein Al/dimyristoylphosphatidylcholine (apoAl/DMPC) on apolipoprotein B (apoB) secretion and uptake of 125I-labeled low-density lipoproteins (LDL) by cultured human hepatoma HepG2 cells was studied. Addition of different concentrations of exogenous cholesterol (as ethanol solution) to the cells increased apoB secretion and inhibited 1 25I-labelled LDL uptake in a dose-dependent manner. Similar effects were found when the cells were incubated with different concentrations of oleic acid, which enhanced the intracellular cholesterol content. The presence of complexes apoAl/DMPC in the culture medium, which decreased the cellular pool of cholesterol, resulted in the opposite effects: apoB secretion was inhibited, while the LDL-receptor activity was stimulated. Significant negative correlation (r = —0.92, p<0.001) was found between apoB secretion and LDL uptake. The obtained data suggest that cholesterol can induce cooperated changes in apoB secretion and LDL-rcceptor activity.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.0000AA