Methodology for processing mastectomy and cryopreservation of breast cancer tissue in a resource- poor setting: A pilot study
There is scarcity of breast cancer tissues derived from women of African origin available for patient - derived xenograft and organoid models. We aim to create a versatile protocol for processing mastectomy and cryopreservation of breast cancer tissue. An immediate collection of breast cancer tissue...
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Published in: | Cryobiology Vol. 97; pp. 179 - 184 |
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Main Authors: | , , , , , , , , , , , , , , , , |
Format: | Journal Article |
Language: | English |
Published: |
Netherlands
Elsevier Inc
01-12-2020
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Subjects: | |
Online Access: | Get full text |
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Summary: | There is scarcity of breast cancer tissues derived from women of African origin available for patient - derived xenograft and organoid models.
We aim to create a versatile protocol for processing mastectomy and cryopreservation of breast cancer tissue.
An immediate collection of breast cancer tissue from mastectomy was bathed in 4 °C HBSS and immediately transferred to 4 °C RPMI1640 containing HEPES, 10% FBS, Streptomycin and Penicillin. Tissues were processed over ice yielding nine samples of cold ischemic time (20–45 min) stored at 3 min interval. Cut samples were transferred into cryovials containing 4 °C cryoprotectant agent (90% FBS +10% Me2SO) before snap -freezing in liquid Nitrogen vapour and final short-term storage in −80 °C Freezer. The histomorphology, tissue and molecular viability were assessed.
The cold ischemic times had no detrimental effect to the nine samples despite being processed in a resource poor setting, hence providing a reproducible and reliable protocol. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0011-2240 1090-2392 |
DOI: | 10.1016/j.cryobiol.2020.05.006 |