Effectiveness of Chemomechanical Preparation with Alternating Use of Sodium Hypochlorite and EDTA in Eliminating Intracanal Enterococcus faecalis Biofilm

Abstract Introduction The elimination of microorganisms from root canals is a critical step in endodontic treatment. We aimed to evaluate the antimicrobial effectiveness of an alternating irrigation regimen during chemomechanical preparation (CMP). Methods During 21 days, root canals of extracted hu...

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Published in:	Journal of endodontics Vol. 36; no. 5; pp. 894 - 898
Main Authors:	Soares, Janir Alves, PhD, Roque de Carvalho, Maria Auxiliadora, MSc, Cunha Santos, Suelleng Maria, MSc, Mendonça, Rafael Mangerotti Castro, Ribeiro-Sobrinho, Antonio Paulino, PhD, Brito-Júnior, Manoel, MSc, Magalhães, Paula Prazeres, PhD, Santos, Maria Helena, PhD, de Macêdo Farias, Luiz, PhD
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-05-2010
Subjects:	Biofilms - drug effects Colony Count, Microbial Dental Pulp Cavity - microbiology Dentistry Edetic Acid - adverse effects Edetic Acid - pharmacology Endocrinology & Metabolism Enterococcus faecalis Enterococcus faecalis - drug effects ethylenediaminetetraacetic acid Humans root canal irrigants Root Canal Irrigants - administration & dosage Root Canal Irrigants - pharmacology Root Canal Preparation - instrumentation Root Canal Preparation - methods sodium hypochlorite Sodium Hypochlorite - adverse effects Sodium Hypochlorite - pharmacology Enterococcus faecalis root canal irrigants sodium hypochlorite ethylenediaminetetraacetic acid
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Summary:	Abstract Introduction The elimination of microorganisms from root canals is a critical step in endodontic treatment. We aimed to evaluate the antimicrobial effectiveness of an alternating irrigation regimen during chemomechanical preparation (CMP). Methods During 21 days, root canals of extracted human teeth were infected with Enterococcus faecalis , and colonization was confirmed by scanning electron microscopy (SEM). Canals were irrigated with saline solution (control group), with 5.25% NaOCl followed by a final rinse with 17% EDTA (conventional irrigation group), or with the alternating use of NaOCl and EDTA (alternating irrigation [AI] group). Samples were taken before treatment (S1), after CMP (S2), and during the following 14 days. Two specimens/group were analyzed by SEM. Results The AI group yielded negative agar and liquid cultures from immediately after CMP and from the 5th day on, respectively. SEM confirmed several bacterium-free sites in the AI group. Conclusion The irrigation regimen based on the alternating use of NaOCl and EDTA seems to be a promising endodontic tool because it promoted the elimination of root canal E. faecalis biofilms throughout the experimental period.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0099-2399 1878-3554
DOI:	10.1016/j.joen.2010.01.002