Search Results - "Mendez, Joshua H."

Structural basis for substrate selection by the SARS-CoV-2 replicase by Malone, Brandon F., Perry, Jason K., Olinares, Paul Dominic B., Lee, Hery W., Chen, James, Appleby, Todd C., Feng, Joy Y., Bilello, John P., Ng, Honkit, Sotiris, Johanna, Ebrahim, Mark, Chua, Eugene Y. D., Mendez, Joshua H., Eng, Ed T., Landick, Robert, Götte, Matthias, Chait, Brian T., Campbell, Elizabeth A., Darst, Seth A.

“…The SARS-CoV-2 RNA-dependent RNA polymerase coordinates viral RNA synthesis as part of an assembly known as the replication–transcription complex (RTC) 1 …”
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Throughput and resolution with a next-generation direct electron detector by Mendez, Joshua H, Mehrani, Atousa, Randolph, Peter, Stagg, Scott

“…Direct electron detectors (DEDs) have revolutionized cryo-electron microscopy (cryo-EM) by facilitating the correction of beam-induced motion and radiation…”
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Fully automated multi-grid cryoEM screening using Smart Leginon by Cheng, Anchi, Kim, Paul T, Kuang, Huihui, Mendez, Joshua H, Chua, Eugene Y D, Maruthi, Kashyap, Wei, Hui, Sawh, Anjelique, Aragon, Mahira F, Serbynovskyi, Viacheslav, Neselu, Kasahun, Eng, Edward T, Potter, Clinton S, Carragher, Bridget, Bepler, Tristan, Noble, Alex J

“…Single-particle cryo-electron microscopy (cryoEM) is a swiftly growing method for understanding protein structure. With increasing demand for high-throughput,…”
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Characterizing the resolution and throughput of the Apollo direct electron detector by Peng, Ruizhi, Fu, Xiaofeng, Mendez, Joshua H., Randolph, Peter S., Bammes, Benjamin E., Stagg, Scott M.

“…[Display omitted] •A new high-resolution direct electron detection camera, the Apollo, is characterized.•The Apollo uses on-the-fly counting to increase data…”
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Recent advances in infectious disease research using cryo-electron tomography by Asarnow, Daniel, Becker, Vada A, Bobe, Daija, Dubbledam, Charlie, Johnston, Jake D, Kopylov, Mykhailo, Lavoie, Nathalie R, Li, Qiuye, Mattingly, Jacob M, Mendez, Joshua H, Paraan, Mohammadreza, Turner, Jack, Upadhye, Viraj, Walsh, Jr, Richard M, Gupta, Meghna, Eng, Edward T

“…With the increasing spread of infectious diseases worldwide, there is an urgent need for novel strategies to combat them. Cryogenic sample electron microscopy…”
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Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy by Chua, Eugene Y.D, Mendez, Joshua H, Rapp, Micah, Ilca, Serban L, Tan, Yong Zi, Maruthi, Kashyap, Kuang, Huihui, Zimanyi, Christina M, Cheng, Anchi, Eng, Edward T, Noble, Alex J, Potter, Clinton S, Carragher, Bridget

“…Cryo-electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the…”
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Automated pipelines for rapid evaluation during cryoEM data acquisition by Mendez, Joshua H, Chua, Eugene Y D, Paraan, Mohammadreza, Potter, Clinton S, Carragher, Bridget

“…Cryo-electron microscopy (cryoEM) has become a popular method for determining high-resolution structures of biomolecules. However, data processing can be…”
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Assessing the quality of single particle reconstructions by atomic model building by Mendez, Joshua H., Stagg, Scott M.

“…The 2015/2016 Map Challenge challenged cryo-EM practitioners to process a series of publicly available cryo-EM datasets. As part of the challenge, metrics…”
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Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy by Saecker, Ruth M., Mueller, Andreas U., Malone, Brandon, Chen, James, Budell, William C., Dandey, Venkata P., Maruthi, Kashyap, Mendez, Joshua H., Molina, Nina, Eng, Edward T., Yen, Laura Y., Potter, Clinton S., Carragher, Bridget, Darst, Seth A.

“…During formation of the transcription-competent open complex (RPo) by bacterial RNA polymerases (RNAPs), transient intermediates pile up before overcoming a…”
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Processing apoferritin with the Appion pipeline by Stagg, Scott M., Mendez, Joshua H.

“…The 3DEM map challenge provided an opportunity to test different algorithms and workflows for processing single particle cryo-EM data. We were interested in…”
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Cryo-Electron Microscopy Screening Automation across Multiple Grids using Smart Leginon by Sawh-Gopal, Anjelique, Ishemgulova, Aygul, Chua, Eugene Y D, Aragon, Mahira F, Mendez, Joshua H, Eng, Edward T, Noble, Alex J

“…Advancements in cryo-electron microscopy (cryoEM) techniques over the past decade have allowed structural biologists to routinely resolve macromolecular…”
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The first single particle analysis Map Challenge: A summary of the assessments by Heymann, J. Bernard, Marabini, Roberto, Kazemi, Mohsen, Sorzano, Carlos Oscar S., Holmdahl, Maya, Mendez, Joshua H., Stagg, Scott M., Jonic, Slavica, Palovcak, Eugene, Armache, Jean-Paul, Zhao, Jianhua, Cheng, Yifan, Pintilie, Grigore, Chiu, Wah, Patwardhan, Ardan, Carazo, Jose-Maria

“…The recent successes of cryo-electron microscopy fostered great expectation of solving many new and previously recalcitrant biomolecular structures. However,…”
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Photoreception and signaling in bacterial phytochrome revealed by single-particle cryo-EM by Malla, Tek Narsingh, Hernandez, Carolina, Muniyappan, Srinivasan, Menendez, David, Bizhga, Dorina, Mendez, Joshua H, Schwander, Peter, Stojković, Emina A, Schmidt, Marius

“…Phytochromes are red-light photoreceptors discovered in plants with homologs in bacteria and fungi that regulate a variety of physiological responses. They…”
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Characterization of the DE64 Direct Electron Detector by Mendez, Joshua H., Stagg, Scott M.

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Throughput and Resolution with a Next Generation Direct Detector by Stagg, Scott M., Mendez, Joshua H.

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Improving photon detector efficiency using a high-fidelity optical CNOT gate by Brown, Katherine L, Singh, Robinjeet, Plaskus, Joshua H. Mendez, Broadus, Hanna E, Dowling, Jonathan P

“…Phys. Rev. A 91, 022327 (2015) A significant problem for optical quantum computing is inefficient, or inaccurate photo-detectors. It is possible to use CNOT…”
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Improving photon detector efficiency using a high-fidelity optical controlled-not gate by Brown, Katherine L., Singh, Robinjeet, Plaskus, Joshua H. Mendez, Broadus, Hanna E., Dowling, Jonathan P.

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