Search Results - "Memelova, L. V."

Synthesis of mixed ribo/deoxyribopolynucleotides by mutant T7 RNA polymerase by Gudima, S.O, Kostyuk, D.A, Grishchenko, O.I, Tunitskaya, V.L, Memelova, L.V, Kochetkov, S.N

“…Synthesis of deoxynucleotide-containing RNA-like single-stranded polynucleotides (dcRNAs) using the Y639F, S641A mutant of T7 RNA polymerase (T7 RNAP) was…”
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Photoaffinity Modification of Bacteriophage T7 DNA-Dependent RNA Polymerase with a Reaction Product Containing an Azido Derivative of UTP by Tunitskaya, V. L., Memelova, L. V., Skoblov, Yu. S., Kochetkov, S. N.

“…The possibility of using 5-[3-(E)-(4-azido-2,3,5,6-tetrafluorobenzamido)-propenyl-1]-UTP (N^sub 3^-TFBP-UTP) as a photoaffinity modifier of T7 DNA-dependent…”
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Mutant T7 RNA polymerase is capable of catalyzing DNA primer extension reaction by Rusakova, E.E, Tunitskaya, V.L, Memelova, L.V, Kochetkova, S.V, Kostyuk, D.A, Kochetkov, S.N

“…The mutant T7 RNA polymerase (T7 RNAP), containing two substitutions (Y639F, S641A) was earlier shown to utilize both rNTP and dNTP in a transcription-like…”
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The studies of cooperative regions in T7 RNA polymerase by Protasevich, I.I., Memelova, L.V., Kochetkov, S.N., Makarov, A.A.

“…The heat denaturation of bacteriophage T7 RNA polymerase (T7RNAP) was studied by scanning microcalorimetry. The thermodynamic parameters of the denaturation…”
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Photoaffinity modification of bacteriophage T7 DNA-dependent RNA polymerase by the reaction product containing the azido derivative of UTP by Tunitskaia, V L, Memelova, L V, Skoblov, Iu S, Kochetkov, S N

“…The possibility of using the 5-[3-(E)-(4-azido-2,3,5,6-tetrafluorobenzamido)-propenyl-1]-UTP (N3-TFBP-UTP) as the affinity modificator of bacteriophage T7…”
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Mapping of T7 RNA polymerase active site with novel reagents – oligonucleotides with reactive dialdehyde groups by Tunitskaya, Vera L, Rusakova, Ekaterina E, Memelova, Lyudmila V, Kochetkov, Sergey N, Van Aerschot, Arthur, Herdewijn, Piet, Efimtseva, Ekaterina V, Ermolinsky, Boris S, Mikhailov, Sergey N

“…Oligonucleotides of a novel type containing 2′- O-β-ribofuranosyl-cytidine were synthesized and further oxidized to yield T7 consensus promoters with…”
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Deoxyribonucleotide-containing RNAs: A novel class of templates for HIV-1 reverse transcriptase by Gudima, Severin O., Kazantseva, Ekaterina G., Kostyuk, Dmitry A., Shchaveleva, Irina L., Grishchenko, Olga I., Memelova, Lyudmila V., Kochetkov, Sergey N.

“…Deoxyribonucleotide-containing RNA-like polynucleotides (dcRNAs) were synthesized by mutant T7 RNA polymerase and their structures confirmed by sequencing…”
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Investigation of the sites phosphorylated in lysine-rich histones by protein kinase from pig brain by Shlyapnikov, S.V., Arutyunyan, A.A., Kurochkin, S.N., Memelova, L.V., Nesterova, M.V., Sashchenko, L.P., Severin, E.S.

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Identification of the exposed and buried cysteine residues in the peptide sequence of aspartate aminotransferase from pig heart cytosol by Zufarova, R A, Dedyukina, M M, Memelova, L V, Torchinsky, Y M

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Inactivation of bacteriophage T7 DNA-dependent RNA polymerase by 5'-p-fluorosulfonylbenzoyladenosine : identification of the modification site and the effect of the modification on enzyme action by TUNITSKAYA, V. L, AKBAROV, A. K, LUCHIN, S. V, MEMELOVA, L. V, RECHINSKY, V. O, KOCHETKOV, S. N

“…Bacteriophage T7 RNA polymerase was covalently modified by 5'-[4-fluorosulfonyl)benzoyl]adenosine (4-FSO2BzAdo). The modified enzyme lacks the ability to…”
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Site-specific mutagenesis of RNA-polymerase from bacteriophage T7: effect of Met-635 and Ser-633 substitutions on enzyme properties by Rusakova, E E, Iankin, A P, Memelova, L V, Tunitskaia, V L, Kochetkov, S N

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Regularities in utilization of deoxyribonucleoside triphosphates by mutant forms of bacteriophage T7 RNA polymerase by Tunitskaia, V L, Memelova, L V, Kostiuk, D A, Gudima, S O, Kochetkov, S N

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Stability of human immunodeficiency virus to azidothymidine. II. Kinetic characteristics of "AZT-resistant" mutant forms of reverse transcriptase by Pokholok, D K, Gudima, S O, Memelova, L V, Esipov, D S, Rechinskiĭ, V O, Kochetkov, S N

“…Prolonged treatment of AIDS patients with azidothymidine results in the development of resistance to the drug which correlates with the appearance of point…”
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Kinetic analysis of interaction of human immunodeficiency virus reverse transcriptase with alkaloids by Gudima, S O, Memelova, L V, Borodulin, V B, Pokholok, D K, Mednikov, B M, Tolkachev, O N, Kochetkov, S N

“…The interactions of HIV-I reverse transcriptase with some alkaloids were studied. Among nine compounds tested three--berberine, palmatine and…”
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Studies of the resistance of the human immunodeficiency virus to azidothymidine. I. Kinetic studies of the interaction between reverse transcriptase and a series of its mutant forms with substrates and azidothymidine-5'-trisphosphate by Pokholok, D K, Gudima, S O, Esipov, D S, Dobrynin, V N, Memelova, L V, Rechinskiĭ, V O, Kochetkov, S N

“…Prolonged therapy of AIDS patients with azidothymidine results in the development of resistance to the drug. This phenomenon is accompanied by the appearance…”
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Functional studies of mutant forms of bacteriophage T7 RNA polymerases containing point substitutions in the motif at the active site of the enzyme by Tunitskaia, V L, Dragan, S M, Kostiuk, D A, Liakhov, D L, Memelova, L V, Rechinskiĭ, V O, Kochetkov, S N

“…Monomeric DNA and RNA polymerases contain a number of conserved motifs. By means of random and oligonucleotide-directed mutagenesis the point mutants of…”
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Affinity modification of DNA-dependent RNA-polymerase from phage T7 with 5'-p-fluorosulfonylbenzoyl adenosine: the effect of modification on the interaction with substrates by Tunitskaia, V L, Luchin, S V, Memelova, L V, Liakhov, D L, Rechinskiĭ, V O, Kochetkov, S N

“…T7 RNA polymerase, covalently modified with 5'-p-fluorosulfonylbenzoyl adenosine, looses the ability of binding the promoter (pGEM-2 plasmid) and poly(dC)…”
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Activation of adenylate cyclase system enzymes and lysosomal acid phosphatase in target cells interacting with T-killer cells by Sashchenko, L P, Kupriianova, T A, Memelova, L V, Bykovskaia, S N, Gnuchev, N V

“…The interaction of T-killers with target cells was studied to reveal the biochemical changes in the latter. On specific binding of target cells with T-killers…”
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Phosphorylation of lysine-rich histones by swine brain histokinase by Shliapnikov, S V, Arutiunian, A A, Kurochkin, S N, Memelova, L V, Nesterova, M V

“…The phosphorylation of lysine-rich histones F1, F2a2 and F2b of calf thymus has been investigated using homogeneous histone kinase from pig brain. 32P-labelled…”
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Identification of cysteine residues in aspartate transaminase from pig-heart cytosol by Zufarova, R.A, Dedyukina, M.M, Memelova, L.V

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