Egg data validation in quantitative monitoring of Aedes albopictus in Emilia-Romagna region, Italy

Egg data validation in quantitative monitoring of Aedes albopictus in Emilia-Romagna region, Italy

The monitoring of Aedes urban species is usually conducted by ovitraps, which can provide a good estimate of population density at low cost and relatively easy management. When the monitoring network is managed by many operators, as in the case of the Emilia-Romagna regional plan, it becomes necessa...

Full description

Saved in:
Bibliographic Details
Published in:Pathogens and global health Vol. 115; no. 2; pp. 125 - 131
Main Authors: Carrieri, Marco, Albieri, Alessandro, Gentili, Lisa, Bacchi, Marta, Manzieri, Anna Maria, Angelini, Paola, Venturelli, Claudio, Matrangolo, Carmela, Leis, Marilena, Pezzi, Marco, Rani, Monica, Iezzi, Rocco Silvio, Melotti, Sabrina, Casari, Alice, Bellini, Romeo
Format: Journal Article
Language:English
Published: England Taylor & Francis 17-02-2021
Subjects:
Aedes
Animals
Chikungunya
dengue
Italy
Mosquito Control
Ovum
quality assessment
quality control
zika
Italy
Chikungunya
quality control
zika
dengue
quality assessment
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The monitoring of Aedes urban species is usually conducted by ovitraps, which can provide a good estimate of population density at low cost and relatively easy management. When the monitoring network is managed by many operators, as in the case of the Emilia-Romagna regional plan, it becomes necessary to develop a quality control methodology capable of highlighting the possible data incoherency and ensuring that the monitoring system provides reliable data. This paper presents the procedure applied in the egg counting phase as developed in Emilia-Romagna in order to check the data quality and validate the data before being included in the database. Several steps have been identified and protocols developed to serve quality control such as training of technicians and intralaboratory quality check, daily random double counting of Masonite paddles and interlaboratory periodic comparison. The qualitative test showed that all operators were able to distinguish Aedes albopictus eggs from other mosquito species eggs. The quantitative test showed significant differences between the 11 operators, with a relative error resulting in the range of 0.1-25.8%. The daily internal double counting of randomly extracted Masonite slides was performed by the coordinator showing a relative error in the range 2.47-2.63% without statistical differences, confirming a good alignment of the operators with the coordinator throughout the monitoring period. The interlaboratory comparison trial produced an average relative error of 7.20% showing a sufficient alignment between the three laboratories. Moreover a new time-saving methods in egg counting was developed and tested in real condition.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2047-7724
2047-7732
DOI:10.1080/20477724.2020.1866375