Toxicological, biochemical, and histopathological evaluation of rats fed with macrofungal-treated cottonseed cake

Gossypol is a terpene-polyphenolic compound specific to cotton plants. Considered as an anti-nutritional factor, monogastric livestock show greater sensitivity to this metabolite than ruminants. The aim of this investigation was to evaluate the toxicological effects of gossypol-containing and biodet...

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Bibliographic Details
Published in:	Frontiers in animal science Vol. 5
Main Authors:	Deboleto, Sandriele Goes de Campos, do Nascimento, Camila Aparecida, Melo, Jean Carlos Souza, Conceição, Aparecido Almeida, Araujo, Ana Paula Fernandes, Neto, Clemente Batista Soares, Miller, Robert Neil Gerard, de Siqueira, Felix Gonçalves, Mendonça, Simone, Honorato, Claucia Aparecida
Format:	Journal Article
Language:	English
Published:	Frontiers Media S.A 18-04-2024
Subjects:	biodetoxification cottonseed cake edible mushroom Free-Gossypol solid fermentation
Online Access:	Get full text
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Summary:	Gossypol is a terpene-polyphenolic compound specific to cotton plants. Considered as an anti-nutritional factor, monogastric livestock show greater sensitivity to this metabolite than ruminants. The aim of this investigation was to evaluate the toxicological effects of gossypol-containing and biodetoxified cottonseed-cake on hepatic function in rats. Biodetoxification of cottonseed cake was performed by the macrofungi Fistulina hepatica BRM 047114 and Pleurotus ostreatus BRM 055505 in a solid-state cultivation process. A toxicological study was performed using albino Wistar rat males ( Rattus norvergicus ). A feed assay was conducted using the macrofungi cultivated on cottonseed cake (5%, 15%, and 25%). Animal growth, feed conversion, hemogram, serum biochemical analyses and histopathology were evaluated. For biodetoxified cottonseed cake treatments with both macrofungi, concentrations of feed up to 15% resulted in animal development similar to that of the untreated group. Histopathological analysis after feeding with biodetoxified cottonseed cake presented an unaltered cordonal arrangement. Nevertheless, the group fed with cottonseed cake biodetoxified using F. hepatica BRM 047114 showed discrete inflammatory infiltrate, vacuolization, and pyknotic nuclei in hepatocytes. P. ostreatus BRM 055505 resulted in efficient reduction in toxic effects of free gossypol, with regular animal growth, maintenance of normal liver activities, and a reduction in circulating cholesterol levels. In conclusion, P. ostreatus and F. hepatica were able to minimize the toxic effects of free gossypol in rat diets, representing an important contribution for feed-formulation development for non-ruminants.
ISSN:	2673-6225 2673-6225
DOI:	10.3389/fanim.2024.1343680