In situ electrochemical and AFM study of thalidomide–DNA interaction

In situ electrochemical and AFM study of thalidomide–DNA interaction

The interaction of thalidomide (TD) with double-stranded DNA (dsDNA) was studied using atomic force microscopy (AFM) at highly oriented pyrolytic graphite (HOPG), differential pulse voltammetry (DPV) at glassy carbon electrodes (GCE), UV–Vis and electrophoresis. After incubation of dsDNA with differ...

Full description

Saved in:
Bibliographic Details
Published in:Bioelectrochemistry (Amsterdam, Netherlands) Vol. 76; no. 1; pp. 201 - 207
Main Authors: Oliveira, S.C.B., Chiorcea-Paquim, A.M., Ribeiro, S.M., Melo, A.T.P., Vivan, M., Oliveira-Brett, A.M.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-09-2009
Subjects:
AFM
Animals
Carbon - chemistry
Cattle
DNA - chemistry
DNA - metabolism
DNA - ultrastructure
DNA-damage
Electrochemistry
Electrodes
Electrophoresis
Glass - chemistry
Magnetics
Microscopy, Atomic Force
Oxidative Stress
Spectrophotometry, Ultraviolet
Thalidomide
Thalidomide - chemistry
Thalidomide - metabolism
Thalidomide–DNA interaction
Voltammetry
AFM
Voltammetry
Thalidomide–DNA interaction
DNA-damage
Thalidomide
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The interaction of thalidomide (TD) with double-stranded DNA (dsDNA) was studied using atomic force microscopy (AFM) at highly oriented pyrolytic graphite (HOPG), differential pulse voltammetry (DPV) at glassy carbon electrodes (GCE), UV–Vis and electrophoresis. After incubation of dsDNA with different concentrations of TD, the AFM images show the formation of thin and incomplete TD–DNA network films with a number of embedded molecular aggregates and regions of uncovered HOPG. Both the TD–dsDNA aggregates and network thickness directly depended on the TD concentration and incubation time. The voltammetric data also showed that the modifications caused by TD to the DNA double helical structure are time-dependent. In agreement with AFM, DPV, UV–Vis and electrophoresis results, a model is proposed for the TD–DNA interaction, considering that TD intercalates into the dsDNA, causing defects in the dsDNA secondary structure and DNA double helix unwinding. Moreover, both AFM and DPV show that condensation is caused to DNA by TD and occurs until 24 h of incubation, as well as DNA oxidative damage, detected electrochemically by the appearance of the 8-oxoGua and/or 2,8 oxoAde oxidation peak.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1567-5394
1878-562X
DOI:10.1016/j.bioelechem.2009.03.003