Search Results - "Mello, Craig C."

Melting dsDNA Donor Molecules Greatly Improves Precision Genome Editing in Caenorhabditis elegans by Ghanta, Krishna S, Mello, Craig C

“…Abstract Melting and fast cooling double stranded DNA donor molecules prior to injection dramatically increases the frequency of homology-directed repair for…”
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piRNAs Initiate an Epigenetic Memory of Nonself RNA in the C. elegans Germline by Shirayama, Masaki, Seth, Meetu, Lee, Heng-Chi, Gu, Weifeng, Ishidate, Takao, Conte, Darryl, Mello, Craig C.

“…Organisms employ a fascinating array of strategies to silence invasive nucleic acids such as transposons and viruses. Although evidence exists for several…”
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C. elegans piRNAs Mediate the Genome-wide Surveillance of Germline Transcripts by Lee, Heng-Chi, Gu, Weifeng, Shirayama, Masaki, Youngman, Elaine, Conte, Darryl, Mello, Craig C.

“…Piwi Argonautes and Piwi-interacting RNAs (piRNAs) mediate genome defense by targeting transposons. However, many piRNA species lack obvious sequence…”
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The C. elegans CSR-1 Argonaute Pathway Counteracts Epigenetic Silencing to Promote Germline Gene Expression by Seth, Meetu, Shirayama, Masaki, Gu, Weifeng, Ishidate, Takao, Conte, Darryl, Mello, Craig C.

“…Organisms can develop adaptive sequence-specific immunity by reexpressing pathogen-specific small RNAs that guide gene silencing. For example, the C. elegans…”
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The RNase PARN-1 Trims piRNA 3′ Ends to Promote Transcriptome Surveillance in C. elegans by Tang, Wen, Tu, Shikui, Lee, Heng-Chi, Weng, Zhiping, Mello, Craig C.

“…Piwi-interacting RNAs (piRNAs) engage Piwi proteins to suppress transposons and are essential for fertility in diverse organisms. An interesting feature of…”
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CapSeq and CIP-TAP Identify Pol II Start Sites and Reveal Capped Small RNAs as C. elegans piRNA Precursors by Gu, Weifeng, Lee, Heng-Chi, Chaves, Daniel, Youngman, Elaine M., Pazour, Gregory J., Conte, Darryl, Mello, Craig C.

“…Piwi-interacting (pi) RNAs are germline-expressed small RNAs linked to epigenetic programming. C. elegans piRNAs are thought to be transcribed as independent…”
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Identification of piRNA Binding Sites Reveals the Argonaute Regulatory Landscape of the C. elegans Germline by Shen, En-Zhi, Chen, Hao, Ozturk, Ahmet R., Tu, Shikui, Shirayama, Masaki, Tang, Wen, Ding, Yue-He, Dai, Si-Yuan, Weng, Zhiping, Mello, Craig C.

“…piRNAs (Piwi-interacting small RNAs) engage Piwi Argonautes to silence transposons and promote fertility in animal germlines. Genetic and computational studies…”
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Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals by Sharma, Upasna, Conine, Colin C., Shea, Jeremy M., Boskovic, Ana, Derr, Alan G., Bing, Xin Y., Belleannee, Clemence, Kucukural, Alper, Serra, Ryan W., Sun, Fengyun, Song, Lina, Carone, Benjamin R., Ricci, Emiliano P., Li, Xin Z., Fauquier, Lucas, Moore, Melissa J., Sullivan, Robert, Mello, Craig C., Garber, Manuel, Rando, Oliver J.

“…Several recent studies link parental environments to phenotypes in subsequent generations. In this work, we investigate the mechanism by which paternal diet…”
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The CERV protein of Cer1, a C. elegans LTR retrotransposon, is required for nuclear export of viral genomic RNA and can form giant nuclear rods by Sun, Bing, Kim, Haram, Mello, Craig C, Priess, James R

“…Retroviruses and closely related LTR retrotransposons export full-length, unspliced genomic RNA (gRNA) for packaging into virions and to serve as the mRNA…”
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Germ granule dysfunction is a hallmark and mirror of Piwi mutant sterility by Spichal, Maya, Heestand, Bree, Billmyre, Katherine Kretovich, Frenk, Stephen, Mello, Craig C., Ahmed, Shawn

“…In several species, Piwi/piRNA genome silencing defects cause immediate sterility that correlates with transposon expression and transposon-induced genomic…”
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Specific miRNA Stabilization by Gld2-Catalyzed Monoadenylation by D’Ambrogio, Andrea, Gu, Weifeng, Udagawa, Tsuyoshi, Mello, Craig C., Richter, Joel D.

“…MicroRNAs (miRNAs) are small, noncoding RNAs that inhibit translation and promote mRNA decay. The levels of mature miRNAs are the result of different rates of…”
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Resistance to Cry14A family Bacillus thuringiensis crystal proteins in Caenornabditis elegans operates via the nhr-31 transcription factor and vacuolar-type ATPase pathway by Kim, Youmie, Nguyen, Thanh-Thanh, Durning, Daniel J, Ishidate, Takao, Aydemir, Ozkan, Mello, Craig C, Hu, Yan, Kahn, Theodore W, Aroian, Raffi V

“…Bacillus thuringiensis (Bt) has been successfully used commercially for more than 60 years for biocontrol of insect pests. Since 1996, transgenic plants…”
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Microinjection for precision genome editing in Caenorhabditis elegans by Ghanta, Krishna S., Ishidate, Takao, Mello, Craig C.

“…In Caenorhabditis elegans, targeted genome editing techniques are now routinely used to generate germline edits. The remarkable ease of C. elegans germline…”
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The nuclear Argonaute HRDE-1 directs target gene re-localization and shuttles to nuage to promote small RNA-mediated inherited silencing by Ding, Yue-He, Ochoa, Humberto J., Ishidate, Takao, Shirayama, Masaki, Mello, Craig C.

“…Argonaute/small RNA pathways and heterochromatin work together to propagate transgenerational gene silencing, but the mechanisms behind their interaction are…”
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A Sex Chromosome piRNA Promotes Robust Dosage Compensation and Sex Determination in C. elegans by Tang, Wen, Seth, Meetu, Tu, Shikui, Shen, En-Zhi, Li, Qian, Shirayama, Masaki, Weng, Zhiping, Mello, Craig C.

“…In metazoans, Piwi-related Argonaute proteins engage piRNAs (Piwi-interacting small RNAs) to defend the genome against invasive nucleic acids, such as…”
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Distinct Argonaute-Mediated 22G-RNA Pathways Direct Genome Surveillance in the C. elegans Germline by Gu, Weifeng, Shirayama, Masaki, Conte, Darryl, Vasale, Jessica, Batista, Pedro J., Claycomb, Julie M., Moresco, James J., Youngman, Elaine M., Keys, Jennifer, Stoltz, Matthew J., Chen, Chun-Chieh G., Chaves, Daniel A., Duan, Shenghua, Kasschau, Kristin D., Fahlgren, Noah, Yates, John R., Mitani, Shohei, Carrington, James C., Mello, Craig C.

“…Endogenous small RNAs (endo-siRNAs) interact with Argonaute (AGO) proteins to mediate sequence-specific regulation of diverse biological processes. Here, we…”
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Suppression of pervasive noncoding transcription in embryonic stem cells by esBAF by Hainer, Sarah J, Gu, Weifeng, Carone, Benjamin R, Landry, Benjamin D, Rando, Oliver J, Mello, Craig C, Fazzio, Thomas G

“…Approximately 75% of the human genome is transcribed, the majority of which does not encode protein. However, many noncoding RNAs (ncRNAs) are rapidly degraded…”
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The Argonaute CSR-1 and Its 22G-RNA Cofactors Are Required for Holocentric Chromosome Segregation by Claycomb, Julie M., Batista, Pedro J., Pang, Ka Ming, Gu, Weifeng, Vasale, Jessica J., van Wolfswinkel, Josien C., Chaves, Daniel A., Shirayama, Masaki, Mitani, Shohei, Ketting, René F., Conte, Darryl, Mello, Craig C.

“…RNAi-related pathways regulate diverse processes, from developmental timing to transposon silencing. Here, we show that in C. elegans the Argonaute CSR-1, the…”
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Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans by Conine, Colin C, Batista, Pedro J, Gu, Weifeng, Claycomb, Julie M, Chaves, Daniel A, Shirayama, Masaki, Mello, Craig C

“…Gametogenesis is a thermosensitive process in numerous metazoans, ranging from worms to man. In Caenorhabditis elegans, a variety of RNA-binding proteins that…”
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PRG-1 and 21U-RNAs Interact to Form the piRNA Complex Required for Fertility in C. elegans by Batista, Pedro J., Ruby, J. Graham, Claycomb, Julie M., Chiang, Rosaria, Fahlgren, Noah, Kasschau, Kristin D., Chaves, Daniel A., Gu, Weifeng, Vasale, Jessica J., Duan, Shenghua, Conte, Darryl, Luo, Shujun, Schroth, Gary P., Carrington, James C., Bartel, David P., Mello, Craig C.

“…In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we…”
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