APETALA2 control of barley internode elongation

Many plants dramatically elongate their stems during flowering, yet how this response is coordinated with the reproductive phase is unclear. We demonstrate that microRNA (miRNA) control of ( ) is required for rapid, complete elongation of stem internodes in barley, especially of the final 'pedu...

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Published in:	Development (Cambridge) Vol. 146; no. 11
Main Authors:	Patil, Vrushali, McDermott, Hannah I, McAllister, Trisha, Cummins, Michael, Silva, Joana C, Mollison, Ewan, Meikle, Rowan, Morris, Jenny, Hedley, Pete E, Waugh, Robbie, Dockter, Christoph, Hansson, Mats, McKim, Sarah M
Format:	Journal Article
Language:	English
Published:	England The Company of Biologists Ltd 12-06-2019
Subjects:	Annan medicinsk bioteknologi APETALA2 Biologi Biological Sciences Cell Biology Cellbiologi Cereal development Intercalary meristem Jasmonate Medical and Health Sciences Medical Biotechnology Medicin och hälsovetenskap Medicinsk bioteknologi Natural Sciences Naturvetenskap Other Medical Biotechnology Phase change Stem elongation APETALA2 Intercalary meristem Jasmonate Cereal development Stem elongation Phase change
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Summary:	Many plants dramatically elongate their stems during flowering, yet how this response is coordinated with the reproductive phase is unclear. We demonstrate that microRNA (miRNA) control of ( ) is required for rapid, complete elongation of stem internodes in barley, especially of the final 'peduncle' internode directly underneath the inflorescence. Disrupted miR172 targeting of in the barley mutant caused lower mitotic activity, delayed growth dynamics and premature lignification in the peduncle leading to fewer and shorter cells. Stage- and tissue-specific comparative transcriptomics between and its parent cultivar showed reduced expression of proliferation-associated genes, ectopic expression of maturation-related genes and persistent, elevated expression of genes associated with jasmonate and stress responses. We further show that applying methyl jasmonate (MeJA) phenocopied the stem elongation of , and that itself was hypersensitive to inhibition by MeJA but less responsive to promotion by gibberellin. Taken together, we propose that miR172-mediated restriction of may modulate the jasmonate pathway to facilitate gibberellin-promoted stem growth during flowering.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Hugh Robson Building, University of Edinburgh, Edinburgh EH8 9XD, Scotland. Present address: Institute of Evolutionary Biology and Centre for Immunity, Infection and Evolution, University of Edinburgh, Edinburgh EH9 3JT, Scotland.
ISSN:	0950-1991 1477-9129 1477-9129
DOI:	10.1242/dev.170373