Search Results - "Mehl, Ryan"

Site-specific dual encoding and labeling of proteins via genetic code expansion by Bednar, Riley M, Karplus, P Andrew, Mehl, Ryan A

“…The ability to selectively modify proteins at two or more defined locations opens new avenues for manipulating, engineering, and studying living systems. As a…”
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Access to Faster Eukaryotic Cell Labeling with Encoded Tetrazine Amino Acids by Jang, Hyo Sang, Jana, Subhashis, Blizzard, Robert J, Meeuwsen, Joseph C, Mehl, Ryan A

“…Labeling of biomolecules in live eukaryotic cells has been limited by low component stability and slow reaction rates. We show that genetically encoded…”
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Ideal Bioorthogonal Reactions Using A Site-Specifically Encoded Tetrazine Amino Acid by Blizzard, Robert J, Backus, Dakota R, Brown, Wes, Bazewicz, Christopher G, Li, Yi, Mehl, Ryan A

“…Bioorthogonal reactions for labeling biomolecules in live cells have been limited by slow reaction rates or low component selectivity and stability. Ideal…”
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Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRET by Wang, Kaihang, Sachdeva, Amit, Cox, Daniel J., Wilf, Nabil M., Lang, Kathrin, Wallace, Stephen, Mehl, Ryan A., Chin, Jason W.

“…The ability to introduce different biophysical probes into defined positions in target proteins will provide powerful approaches for interrogating protein…”
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Increasing Enzyme Stability and Activity through Hydrogen Bond-Enhanced Halogen Bonds by Carlsson, Anna-Carin C, Scholfield, Matthew R, Rowe, Rhianon K, Ford, Melissa Coates, Alexander, Austin T, Mehl, Ryan A, Ho, P. Shing

“…The construction of more stable proteins is important in biomolecular engineering, particularly in the design of biologics-based therapeutics. We show here…”
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Well-defined biohybrids using reversible-deactivation radical polymerization procedures by Averick, Saadyah, Mehl, Ryan A., Das, Subha R., Matyjaszewski, Krzysztof

“…The use of reversible deactivation radical polymerization (RDRP) methods has significantly expanded the field of bioconjugate synthesis. RDRP procedures have…”
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Conformationally Strained trans -Cyclooctene with Improved Stability and Excellent Reactivity in Tetrazine Ligation by Darko, Ampofo, Wallace, Stephen, Dmitrenko, Olga, Machovina, Melodie M, Mehl, Ryan A, Chin, Jason W, Fox, Joseph M

“…Computation has guided the design of conformationally-strained dioxolane-fused -cyclooctene (d-TCO) derivatives that display excellent reactivity in the…”
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Partial wrapping of single-stranded DNA by replication protein A and modulation through phosphorylation by Chadda, Rahul, Kaushik, Vikas, Ahmad, Iram Munir, Deveryshetty, Jaigeeth, Holehouse, Alex S, Sigurdsson, Snorri Th, Biswas, Gargi, Levy, Yaakov, Bothner, Brian, Cooley, Richard B, Mehl, Ryan A, Dastvan, Reza, Origanti, Sofia, Antony, Edwin

“…Single-stranded DNA (ssDNA) intermediates which emerge during DNA metabolic processes are shielded by replication protein A (RPA). RPA binds to ssDNA and acts…”
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1,2,4-Triazines Are Versatile Bioorthogonal Reagents by Kamber, David N, Liang, Yong, Blizzard, Robert J, Liu, Fang, Mehl, Ryan A, Houk, K. N, Prescher, Jennifer A

“…A new class of bioorthogonal reagents, 1,2,4-triazines, is described. These scaffolds are stable in biological media and capable of robust reactivity with…”
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Genetic Encoding of Phosphorylated Amino Acids into Proteins by Allen, Michael C., Karplus, P. Andrew, Mehl, Ryan A., Cooley, Richard B.

“…Reversible phosphorylation is a fundamental mechanism for controlling protein function. Despite the critical roles phosphorylated proteins play in physiology…”
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Structure–Energy Relationships of Halogen Bonds in Proteins by Scholfield, Matthew R, Ford, Melissa Coates, Carlsson, Anna-Carin C, Butta, Hawera, Mehl, Ryan A, Ho, P. Shing

“…The structures and stabilities of proteins are defined by a series of weak noncovalent electrostatic, van der Waals, and hydrogen bond (HB) interactions. In…”
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Cyclopropenones for Metabolic Targeting and Sequential Bioorthogonal Labeling by Row, R. David, Shih, Hui-Wen, Alexander, Austin T, Mehl, Ryan A, Prescher, Jennifer A

“…Cyclopropenones are attractive motifs for bioorthogonal chemistry, owing to their small size and unique modes of reactivity. Unfortunately, the…”
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Biosynthesis and Genetic Encoding of Non-hydrolyzable Phosphoserine into Recombinant Proteins in Escherichia coli by Zhu, Phillip, Mehl, Ryan A, Cooley, Richard B

“…While site-specific translational encoding of phosphoserine (pSer) into proteins in via genetic code expansion (GCE) technologies has transformed our ability…”
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Tyrosine nitration on calmodulin enhances calcium-dependent association and activation of nitric-oxide synthase by Porter, Joseph J., Jang, Hyo Sang, Haque, Mohammad Mahfuzul, Stuehr, Dennis J., Mehl, Ryan A.

“…Production of reactive oxygen species caused by dysregulated endothelial nitric-oxide synthase (eNOS) activity is linked to vascular dysfunction. eNOS is a…”
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A Highly Versatile Expression System for the Production of Multiply Phosphorylated Proteins by Zhu, Phillip, Gafken, Philip R, Mehl, Ryan A, Cooley, Richard B

“…Genetic Code Expansion (GCE) can use TAG stop codons to guide site-specific incorporation of phosphoserine (pSer) into proteins. To eliminate prematurely…”
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Faster Surface Ligation Reactions Improve Immobilized Enzyme Structure and Activity by Chaparro Sosa, Andres F, Bednar, Riley M, Mehl, Ryan A, Schwartz, Daniel K, Kaar, Joel L

“…During integration into materials, the inactivation of enzymes as a result of their interaction with nanometer size denaturing “hotspots” on surfaces…”
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Ultrafast Bioorthogonal Spin-Labeling and Distance Measurements in Mammalian Cells Using Small, Genetically Encoded Tetrazine Amino Acids by Jana, Subhashis, Evans, Eric G. B., Jang, Hyo Sang, Zhang, Shuyang, Zhang, Hui, Rajca, Andrzej, Gordon, Sharona E., Zagotta, William N., Stoll, Stefan, Mehl, Ryan A.

“…Site-directed spin-labeling (SDSL)in combination with double electron–electron resonance (DEER) spectroscopyhas emerged as a powerful technique for…”
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Genetic Incorporation of Two Mutually Orthogonal Bioorthogonal Amino Acids That Enable Efficient Protein Dual-Labeling in Cells by Bednar, Riley M, Jana, Subhashis, Kuppa, Sahiti, Franklin, Rachel, Beckman, Joseph, Antony, Edwin, Cooley, Richard B, Mehl, Ryan A

“…The ability to site-specifically modify proteins at multiple sites in vivo will enable the study of protein function in its native environment with…”
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Accessing isotopically labeled proteins containing genetically encoded phosphoserine for NMR with optimized expression conditions by Vesely, Cat Hoang, Reardon, Patrick N., Yu, Zhen, Barbar, Elisar, Mehl, Ryan A., Cooley, Richard B.

“…Phosphoserine (pSer) sites are primarily located within disordered protein regions, making it difficult to experimentally ascertain their effects on protein…”
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Engineering mutually orthogonal PylRS/tRNA pairs for dual encoding of functional histidine analogues by Taylor, Christopher J., Hardy, Florence J., Burke, Ashleigh J., Bednar, Riley M., Mehl, Ryan A., Green, Anthony P., Lovelock, Sarah L.

“…The availability of an expanded genetic code opens exciting new opportunities in enzyme design and engineering. In this regard histidine analogues have proven…”
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