Impedimetric DNA E-biosensor for multiplexed sensing of Escherichia coli and its virulent f17 strains

An impedance-based DNA multiplexed biosensor was designed to simultaneously detect Escherichia coli ( yaiO gene) and its virulent f17 variant. The thiolated DNA dual probe was self-assembled onto the surface of the gold nanoparticle-modified screen-printed carbon electrode (AuNPs/SPCE) to recognize...

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Bibliographic Details
Published in:	Mikrochimica acta (1966) Vol. 187; no. 11; p. 635
Main Authors:	Rabti, Amal, Zayani, Riham, Meftah, Marwa, Salhi, Imed, Raouafi, Noureddine
Format:	Journal Article
Language:	English
Published:	Vienna Springer Vienna 01-11-2020 Springer Springer Nature B.V
Subjects:	Analysis Analytical Chemistry Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Calves Characterization and Evaluation of Materials Charge transfer Chemistry Chemistry and Materials Science Circuits Deoxyribonucleic acid Detectors DNA DNA sequencing DNA, Bacterial - genetics DNA, Bacterial - isolation & purification E coli Electric Impedance Electric properties Electrochemical Techniques - methods Escherichia coli Escherichia coli - classification Escherichia coli - genetics Escherichia coli - pathogenicity Gene sequencing Genes Genetic aspects Genetic testing Gold Gold - chemistry Linearity Metal Nanoparticles - chemistry Microengineering Multiplexing Nanochemistry Nanoparticles Nanotechnology Nanotechnology devices Nucleotide sequencing Original Paper Regression coefficients Self-assembly Virulence Virulence (Microbiology) Multiplexing Dual biosensor F17 fimbriae Impedance spectroscopy Pathogen Escherichia coli
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Summary:	An impedance-based DNA multiplexed biosensor was designed to simultaneously detect Escherichia coli ( yaiO gene) and its virulent f17 variant. The thiolated DNA dual probe was self-assembled onto the surface of the gold nanoparticle-modified screen-printed carbon electrode (AuNPs/SPCE) to recognize selected sequences from yaiO and f17 genes. The optimal conditions to prepare the bioelectrode were determined based on the monitoring of the impedimetric response fitted to an equivalent electrical circuit model. The charge transfer resistance of the bioelectrode increased by recognizing the target DNA sequences. The limit of detection was 0.8 fM and 1.0 fM for yaiO and f17 target DNA, respectively, and the linearity ranged from 1 × 10 −15 to 1 × 10 −7 M with a linear regression coefficient R ≥ 0.995. The nanodevice provided a novel strategy for simultaneous detection of E. coli and its virulence f17 gene with excellent discrimination with a single-base mismatch, two-base mismatch, and non-complementary sequences. Moreover, genomic DNA extracted from E. coli bacteria isolated from diarrheic camel calves and control animals in Tunisia was successfully detected using the as-prepared biosensor with minimal treatment of the extracted DNA samples. Graphical abstract
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0026-3672 1436-5073
DOI:	10.1007/s00604-020-04614-y