Search Results - "Medina‐Cucurella, Angélica V."

Pro region engineering of nerve growth factor by deep mutational scanning enables a yeast platform for conformational epitope mapping of anti‐NGF monoclonal antibodies by Medina‐Cucurella, Angélica V., Zhu, Yaqi, Bowen, Scott J., Bergeron, Lisa M., Whitehead, Timothy A.

“…Nerve growth factor (NGF) plays a central role in multiple chronic pain conditions. As such, anti‐NGF monoclonal antibodies (mAbs) that function by…”
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Enhanced T cell receptor specificity through framework engineering by Rosenberg, Aaron M, Ayres, Cory M, Medina-Cucurella, Angélica V, Whitehead, Timothy A, Baker, Brian M

“…Development of T cell receptors (TCRs) as immunotherapeutics is hindered by inherent TCR cross-reactivity. Engineering more specific TCRs has proven…”
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A yeast surface display platform for plant hormone receptors: Toward directed evolution of new biosensors by Steiner, Paul J., Bedewitz, Matthew A., Medina‐Cucurella, Angélica V., Cutler, Sean R., Whitehead, Timothy A.

“…Small‐molecule biosensors have major applications in biotechnology and medicine but remain difficult to engineer. Plant hormone receptors represent an…”
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Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds by Beltrán, Jesús, Steiner, Paul J., Bedewitz, Matthew, Wei, Shuang, Peterson, Francis C., Li, Zongbo, Hughes, Brigid E., Hartley, Zachary, Robertson, Nicholas R., Medina-Cucurella, Angélica V., Baumer, Zachary T., Leonard, Alison C., Park, Sang-Youl, Volkman, Brian F., Nusinow, Dmitri A., Zhong, Wenwan, Wheeldon, Ian, Cutler, Sean R., Whitehead, Timothy A.

“…A general method to generate biosensors for user-defined molecules could provide detection tools for a wide range of biological applications. Here, we describe…”
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An orthogonalized PYR1-based CID module with reprogrammable ligand-binding specificity by Park, Sang-Youl, Qiu, Jingde, Wei, Shuang, Peterson, Francis C., Beltrán, Jesús, Medina-Cucurella, Angélica V., Vaidya, Aditya S., Xing, Zenan, Volkman, Brian F., Nusinow, Dmitri A., Whitehead, Timothy A., Wheeldon, Ian, Cutler, Sean R.

“…Plants sense abscisic acid (ABA) using chemical-induced dimerization (CID) modules, including the receptor PYR1 and HAB1, a phosphatase inhibited by…”
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Preferential Identification of Agonistic OX40 Antibodies by Using Cell Lysate to Pan Natively Paired, Humanized Mouse-Derived Yeast Surface Display Libraries by Medina-Cucurella, Angélica, Mizrahi, Rena, Asensio, Michael, Edgar, Robert, Leong, Jackson, Leong, Renee, Lim, Yoong, Nelson, Ayla, Niedecken, Ariel, Simons, Jan, Spindler, Matthew, Stadtmiller, Kacy, Wayham, Nicholas, Adler, Adam, Johnson, David

“…To discover therapeutically relevant antibody candidates, many groups use mouse immunization followed by hybridoma generation or B cell screening. One modern…”
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User-defined single pot mutagenesis using unamplified oligo pools by Medina-Cucurella, Angélica V, Steiner, Paul J, Faber, Matthew S, Beltrán, Jesús, Borelli, Alexandra N, Kirby, Monica B, Cutler, Sean R, Whitehead, Timothy A

“…Abstract User-defined mutagenic libraries are fundamental for applied protein engineering workflows. Here we show that unamplified oligo pools can be used to…”
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Optimization of multi-site nicking mutagenesis for generation of large, user-defined combinatorial libraries by Kirby, Monica B, Medina-Cucurella, Angélica V, Baumer, Zachary T, Whitehead, Timothy A

“…Abstract Generating combinatorial libraries of specific sets of mutations are essential for addressing protein engineering questions involving contingency in…”
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A Potent Recombinant Polyclonal Antibody Therapeutic for Protection Against New Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern by Wayham, Nicholas P, Niedecken, Ariel R, Simons, Jan Fredrik, Chiang, Yao Y, Medina-Cucurella, Angélica V, Mizrahi, Rena A, Wagner, Ellen K, Gras, Ashley, Segal, Ilana, Witte, Peyton, Enstrom, Alexis, Bountouvas, Aristea, Nelson, Sabrina M, Weinberger, Tess, Tan, David, Asensio, Michael A, Subramanian, Alagu, Lim, Yoong Wearn, Adler, Adam S, Keating, Sheila M

“…Abstract Emerging variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) possess mutations that prevent antibody therapeutics from…”
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Pro region engineering of nerve growth factor by deep mutational scanning enables a yeast platform for conformational epitope mapping of anti-NGF monoclonal antibodies by Medina-Cucurella, Angélica V, Zhu, Yaqi, Bowen, Scott J, Bergeron, Lisa M, Whitehead, Timothy A

“…Nerve growth factor (NGF) plays a central role in multiple chronic pain conditions. As such, anti-NGF monoclonal antibodies (mAbs) that function by…”
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Characterizing Protein-Protein Interactions Using Deep Sequencing Coupled to Yeast Surface Display by Medina-Cucurella, Angelica V, Whitehead, Timothy A

“…In this chapter, we discuss a method to determine the affinity and specificity of nearly all single-point mutants for a full-length protein binder. This method…”
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Feline Interleukin-31 Shares Overlapping Epitopes with the Oncostatin M Receptor and IL-31RA by Medina-Cucurella, Angelica V, Bammert, Gary F, Dunkle, William, Javens, Christopher, Zhu, Yaqi, Mutchler, Veronica T, Teel, Janet T, Stein, Caitlin A, Dunham, Steve A, Whitehead, Timothy A

“…Interleukin-31 (IL-31) is a major protein involved in severe inflammatory skin disorders. Its signaling pathway is mediated through two type I cytokine…”
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A Potent Recombinant Polyclonal Antibody Therapeutic for Protection Against New SARS-CoV-2 Variants of Concern by Wayham, Nicholas P, Niedecken, Ariel R, Simons, Jan Fredrik, Chiang, Yao Y, Medina-Cucurella, Angélica V, Mizrahi, Rena A, Wagner, Ellen K, Gras, Ashley, Segal, Ilana, Witte, Peyton, Enstrom, Alexis, Bountouvas, Aristea, Nelson, Sabrina M, Weinberger, Tess, Tan, David, Asensio, Michael A, Subramanian, Alagu, Lim, Yoong Wearn, Adler, Adam S, Keating, Sheila M

“…Emerging variants of SARS-CoV-2 possess mutations that prevent antibody therapeutics from maintaining anti-viral binding and neutralizing efficacy. Monoclonal…”
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Generation of recombinant hyperimmune globulins from diverse B-cell repertoires by Keating, Sheila M., Mizrahi, Rena A., Adams, Matthew S., Asensio, Michael A., Benzie, Emily, Carter, Kyle P., Chiang, Yao, Edgar, Robert C., Gautam, Bishal K., Gras, Ashley, Leong, Jackson, Leong, Renee, Lim, Yoong Wearn, Manickam, Vishal A., Medina-Cucurella, Angelica V., Niedecken, Ariel R., Saini, Jasmeen, Simons, Jan Fredrik, Spindler, Matthew J., Stadtmiller, Kacy, Tinsley, Brendan, Wagner, Ellen K., Wayham, Nicholas, Tracy, LaRee, Lundberg, Carina Vingsbo, Büscher, Dirk, Terencio, Jose Vicente, Roalfe, Lucy, Pearce, Emma, Richardson, Hayley, Goldblatt, David, Ramjag, Anushka T., Carrington, Christine V. F., Simmons, Graham, Muench, Marcus O., Chamow, Steven M., Monroe, Bryan, Olson, Charles, Oguin, Thomas H., Lynch, Heather, Jeanfreau, Robert, Mosher, Rachel A., Walch, Matthew J., Bartley, Christopher R., Ross, Carl A., Meyer, Everett H., Adler, Adam S., Johnson, David S.

“…Plasma-derived polyclonal antibody therapeutics, such as intravenous immunoglobulin, have multiple drawbacks, including low potency, impurities, insufficient…”
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Engineering Deep Sequencing-Guided Platforms to Evaluate Sequence-Function Relationships between Proteins for the Development of Therapeutic Antibodies by Medina-Cucurella, Angelica V

“…Over the past two decades, monoclonal antibodies (mAbs) have been used as a major class of therapeutic treatments for cancer and autoimmune diseases given…”
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GMP Manufacturing and IND-Enabling Studies of a Recombinant Hyperimmune Globulin Targeting SARS-CoV-2 by Mizrahi, Rena A, Lin, Wendy Y, Gras, Ashley, Niedecken, Ariel R, Wagner, Ellen K, Keating, Sheila M, Ikon, Nikita, Manickam, Vishal A, Asensio, Michael A, Leong, Jackson, Medina-Cucurella, Angelica V, Benzie, Emily, Carter, Kyle P, Chiang, Yao, Edgar, Robert C, Leong, Renee, Lim, Yoong Wearn, Simons, Jan Fredrik, Spindler, Matthew J, Stadtmiller, Kacy, Wayham, Nicholas, Büscher, Dirk, Terencio, Jose Vicente, Germanio, Clara Di, Chamow, Steven M, Olson, Charles, Pino, Paula A, Park, Jun-Gyu, Hicks, Amberlee, Ye, Chengjin, Garcia-Vilanova, Andreu, Martinez-Sobrido, Luis, Torrelles, Jordi B, Johnson, David S, Adler, Adam S

“…Conventionally, hyperimmune globulin drugs manufactured from pooled immunoglobulins from vaccinated or convalescent donors have been used in treating…”
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