Mapping the proteome of poplar and application to the discovery of drought-stress responsive proteins

Poplar is the first forest tree genome to be decoded. As an initial step to the comprehensive analysis of poplar proteome, we described reference 2‐D‐maps for eight tissues/organs of the plant, and the functional characterization of some proteins. A total of 398 proteins were excised from the gels....

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Bibliographic Details
Published in:	Proteomics (Weinheim) Vol. 6; no. 24; pp. 6509 - 6527
Main Authors:	Plomion, Christophe, Lalanne, Céline, Claverol, Stéphane, Meddour, Hakim, Kohler, Annegret, Bogeat-Triboulot, Marie-Béatrice, Barre, Aurélien, Le Provost, Grégoire, Dumazet, Hélène, Jacob, Daniel, Bastien, Catherine, Dreyer, Erwin, de Daruvar, Antoine, Guehl, Jean-Marc, Schmitter, Jean-Marie, Martin, Francis, Bonneu, Marc
Format:	Journal Article
Language:	English
Published:	Weinheim WILEY-VCH Verlag 01-12-2006 WILEY‐VCH Verlag Wiley-VCH Wiley-VCH Verlag
Subjects:	2-DE Analytical, structural and metabolic biochemistry Biochemistry, Molecular Biology Biological and medical sciences Databases, Protein Disasters Down-Regulation Drought Electrophoresis, Gel, Two-Dimensional Fundamental and applied biological sciences. Psychology Life Sciences Miscellaneous Oligonucleotide Array Sequence Analysis Peptide Mapping - methods Peptide sequence tag Plant Leaves - chemistry Plant Leaves - genetics Plant Leaves - metabolism Plant Proteins - genetics Plant Proteins - isolation & purification Plant Proteins - metabolism Plant Roots - chemistry Plant Roots - genetics Plant Roots - metabolism PMF Populus - chemistry Populus - genetics Populus - metabolism Proteins Proteomics - methods Proteomics - statistics & numerical data Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tandem Mass Spectrometry Transcription, Genetic Up-Regulation Peptides Proteomics abase / Drought / PMF / Peptide sequence tag Proteome 2-DE Sequence tagged site Protein Stress database peptide sequence tag drought base de donnée PMF tag
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Summary:	Poplar is the first forest tree genome to be decoded. As an initial step to the comprehensive analysis of poplar proteome, we described reference 2‐D‐maps for eight tissues/organs of the plant, and the functional characterization of some proteins. A total of 398 proteins were excised from the gels. About 91.2% were identified by nanospray LC‐MS/MS, based on comparison with 260 000 Populus sp. ESTs. In comparison, reliable PMFs were obtained for only 51% of the spots by MALDI‐TOF‐MS, from which 43% (83 spots) positively matched gene models of the Populus trichocarpa genome sequence. Among these 83 spots, 58% matched with the same proteins as identified by LC‐MS/MS, 21.7% with unknown function proteins and 19.3% with completely different functions. In the second phase, we studied the effect of drought stress on poplar root and leaf proteomes. The function of up‐ and down‐regulated proteins is discussed with respect to the physiological response of the plants and compared with transcriptomic data. Some important clues regarding the way poplar copes with water deficit were revealed.
Bibliography:	istex:8890A0530300C2ABF278575B6044FD1571501BBD ArticleID:PMIC200600362 INRA EFPA ark:/67375/WNG-QXQQQKZQ-1 ANR Institut Fédérateur de Recherches IFR103 Fonds Européens de Développement Régional; FEDER Number: 2003227 Région Aquitaine - No. 20030304002FA INRA, Région Lorraine and the European Commission through the Institut Fédérateur de Recherche IFR110 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1615-9853 1615-9861
DOI:	10.1002/pmic.200600362