The subgingival microbiomes in periodontitis and health of individuals with rheumatoid arthritis and at risk of developing rheumatoid arthritis

The subgingival microbiomes in periodontitis and health of individuals with rheumatoid arthritis and at risk of developing rheumatoid arthritis

Serum anti-citrullinated protein antibodies (ACPAs), present in 70% of people with rheumatoid arthritis (RA), can be detected ≤10years before the onset of clinical disease. RA and periodontitis are epidemiologically associated and we have reported a high incidence of periodontitis in people who are...

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Bibliographic Details
Published in:Journal of oral microbiology Vol. 9; no. sup1; pp. 1325216 - 1
Main Authors: Cheng, Z., Do, T., Mankia, K., Meade, J.L., Hunt, L., Nam, J., Tugnait, A., Speirs, A., Clerehugh, V., Emery, P., Devine, D.
Format: Journal Article
Language:English
Published: Abingdon Taylor & Francis 31-05-2017
Taylor & Francis Ltd
Taylor & Francis Group
Subjects:
Abstract
Actinobacteria
Aggregatibacter actinomycetemcomitans
Antibodies
Arthritis
Autoantibodies
Bacteria
Bacteroidetes
Citrulline
Data processing
Deoxyribonucleic acid
DNA
Epidemiology
Gum disease
Microbiomes
Periodontitis
Proteins
Radioallergosorbent test
Relative abundance
Rheumatoid arthritis
Risk management
Session-3: Periodontal and peri-implant diseases
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Description
Summary:Serum anti-citrullinated protein antibodies (ACPAs), present in 70% of people with rheumatoid arthritis (RA), can be detected ≤10years before the onset of clinical disease. RA and periodontitis are epidemiologically associated and we have reported a high incidence of periodontitis in people who are ACPA+ and at risk of RA. Periodontal bacteria may contribute by multiple routes to the generation of RA-autoantibodies. This study aims to characterise the subgingival microbiomes from periodontitis and health in individuals with/without RA and at risk of RA. Forty-five ACPA+ no RA (RA-at-risk; RAR), 31 healthy controls (HC) and 30 ACPA+ RA patients (RA) underwent a periodontal examination. DNA from subgingival plaque from healthy and deep pocket sites were paired-end sequenced using the Illumina HiSeq3000 and data analysed using MG-RAST + DESeq. Metagenomes in RA samples had high proportions of Actinobacteria; RAR microbiomes contained higher proportions of Bacteroidetes than HC. The relative abundance of P. gingivalis was high in periodontitis and RAR; Aggregatibacter actinomycetemcomitans was detected with similar frequency in each group. Other bacteria implicated in periodontitis and/or autoantibody generation (Filifactor alocis, Prevotella spp, Leptotrichia spp.) were detected. Analyses are on-going to elucidate the diversity and functional potential of the subgingival microbiome associated with RA.
ISSN:2000-2297
2000-2297
DOI:10.1080/20002297.2017.1325216