Optimization and characterization of immobilized E. coli for engineered thermostable xylanase excretion and cell viability

There are many parameters that may have influenced the properties of cell during immobilization process. Particularly, the immobilization methods, carrier materials, and enzyme loading amount that have been proved to be important for immobilization process. The physiological responses of microorgani...

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Bibliographic Details
Published in:	Arabian journal of chemistry Vol. 15; no. 6; p. 103803
Main Authors:	Nor Ashikin, Nur Atiqah Lyana, Mohd Fuzi, Siti Fatimah Zaharah, Abdul Manaf, Shoriya Aruni, Abdul Manas, Nor Hasmaliana, Md Shaarani@ Md Nawi, Shalyda, Md Illias, Rosli
Format:	Journal Article
Language:	English
Published:	Elsevier B.V 01-06-2022 Elsevier
Subjects:	Engineered E. coli Graphene oxide Immobilization Optimization Thermostable enzyme Immobilization Graphene oxide Thermostable enzyme Engineered E. coli Optimization
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Summary:	There are many parameters that may have influenced the properties of cell during immobilization process. Particularly, the immobilization methods, carrier materials, and enzyme loading amount that have been proved to be important for immobilization process. The physiological responses of microorganisms are depending on the immobilization technique used. Typical alterations to the micro-environment of the immobilized cell involved the altered water activity, presence of ionic charges, cell confinement and modified surface tension. In this study, the graphene oxide was selected as a suitable carrier for immobilization process of recombinant E.coli and adsorption was chosen as an appropriate method to improve the production of engineered thermostable xylanase. High level production of thermostable xylanase by immobilized recombinant cell in the 5 L bioreactor was studied by using optimum research surface methodology (RSM) conditions was studied. The immobilization of E. coli onto nanoparticle matrix manages to improve the cell performance by improving the protein expression, reduced the occurrences of cell lysis as well as improved the plasmid stability of the host cell. Thus, immobilization contributes a physical support for both whole cells as well as enzymes to develop a better operative achievement system for industrialized fields and give rise to the biological advancement existing enzyme for instance xylanase.
ISSN:	1878-5352 1878-5379
DOI:	10.1016/j.arabjc.2022.103803