Search Results - "Mcnally, James G."

Single-molecule analysis of transcription factor binding at transcription sites in live cells by Morisaki, Tatsuya, Müller, Waltraud G., Golob, Nicole, Mazza, Davide, McNally, James G.

“…Although numerous live-cell measurements have shown that transcription factors (TFs) bind chromatin transiently, no measurements of transient binding have been…”
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Regulation of RNA polymerase II activation by histone acetylation in single living cells by Stasevich, Timothy J., Hayashi-Takanaka, Yoko, Sato, Yuko, Maehara, Kazumitsu, Ohkawa, Yasuyuki, Sakata-Sogawa, Kumiko, Tokunaga, Makio, Nagase, Takahiro, Nozaki, Naohito, McNally, James G., Kimura, Hiroshi

“…The interplay of histone acetylation and RNA polymerase II activity is investigated using fluorescence microscopy; acetylation of H3 at Lys 27 enhances the…”
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Minimizing the Impact of Photoswitching of Fluorescent Proteins on FRAP Analysis by Mueller, Florian, Morisaki, Tatsuya, Mazza, Davide, McNally, James G.

“…Fluorescence recovery after photobleaching (FRAP) is a widely used imaging technique for measuring the mobility of fluorescently tagged proteins in living…”
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FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know? by Mueller, Florian, Mazza, Davide, Stasevich, Timothy J, McNally, James G

“…The binding of nuclear proteins to chromatin in live cells has been analyzed by kinetic modeling procedures applied to experimental data from fluorescence…”
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Unraveling heme detoxification in the malaria parasite by in situ correlative X-ray fluorescence microscopy and soft X-ray tomography by Kapishnikov, Sergey, Grolimund, Daniel, Schneider, Gerd, Pereiro, Eva, McNally, James G., Als-Nielsen, Jens, Leiserowitz, Leslie

“…A key drug target for malaria has been the detoxification pathway of the iron-containing molecule heme, which is the toxic byproduct of hemoglobin digestion…”
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Photoswitching-free FRAP analysis with a genetically encoded fluorescent tag by Morisaki, Tatsuya, McNally, James G

“…Fluorescence recovery after photobleaching (FRAP) is a widely used imaging technique for measuring protein dynamics in live cells that has provided many…”
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Measuring Chromatin Interaction Dynamics on the Second Time Scale at Single-Copy Genes by Poorey, Kunal, Viswanathan, Ramya, Carver, Melissa N., Karpova, Tatiana S., Cirimotich, Shana M., McNally, James G., Bekiranov, Stefan, Auble, David T.

“…The chromatin immunoprecipitation (ChIP) assay is widely used to capture interactions between chromatin and regulatory proteins, but it is unknown how stable…”
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Analysis of Binding Reactions by Fluorescence Recovery after Photobleaching by Sprague, Brian L., Pego, Robert L., Stavreva, Diana A., McNally, James G.

“…Fluorescence recovery after photobleaching (FRAP) is now widely used to investigate binding interactions in live cells. Although various idealized solutions…”
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Changes in Chromatin Structure and Mobility in Living Cells at Sites of DNA Double-Strand Breaks by Kruhlak, Michael J., Celeste, Arkady, Dellaire, Graham, Fernandez-Capetillo, Oscar, Müller, Waltraud G., McNally, James G., Bazett-Jones, David P., Nussenzweig, André

“…The repair of DNA double-strand breaks (DSBs) is facilitated by the phosphorylation of H2AX, which organizes DNA damage signaling and chromatin remodeling…”
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Cross-Validating FRAP and FCS to Quantify the Impact of Photobleaching on In Vivo Binding Estimates by Stasevich, Timothy J., Mueller, Florian, Michelman-Ribeiro, Ariel, Rosales, Tilman, Knutson, Jay R., McNally, James G.

“…Binding can now be quantified in live cells, but the accuracy of such measurements remains uncertain. To address this uncertainty, we compare fluorescence…”
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Prion Induction by the Short-Lived, Stress-Induced Protein Lsb2 Is Regulated by Ubiquitination and Association with the Actin Cytoskeleton by Chernova, Tatiana A., Romanyuk, Andrey V., Karpova, Tatiana S., Shanks, John R., Ali, Moiez, Moffatt, Nela, Howie, Rebecca L., O'Dell, Andrew, McNally, James G., Liebman, Susan W., Chernoff, Yury O., Wilkinson, Keith D.

“…Yeast prions are self-perpetuating, QN-rich amyloids that control heritable traits and serve as a model for mammalian amyloidoses. De novo prion formation by…”
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Dissecting the binding mechanism of the linker histone in live cells: an integrated FRAP analysis by Stasevich, Timothy J, Mueller, Florian, Brown, David T, McNally, James G

“…The linker histone H1 has a fundamental role in DNA compaction. Although models for H1 binding generally involve the H1 C‐terminal tail and sites S1 and S2…”
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Direct Measurement of Association and Dissociation Rates of DNA Binding in Live Cells by Fluorescence Correlation Spectroscopy by Michelman-Ribeiro, Ariel, Mazza, Davide, Rosales, Tilman, Stasevich, Timothy J., Boukari, Hacene, Rishi, Vikas, Vinson, Charles, Knutson, Jay R., McNally, James G.

“…Measurement of live-cell binding interactions is vital for understanding the biochemical reactions that drive cellular processes. Here, we develop,…”
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Concurrent Fast and Slow Cycling of a Transcriptional Activator at an Endogenous Promoter by Karpova, Tatiana S, Kim, Min J, Spriet, Corentin, Nalley, Kip, Stasevich, Timothy J, Kherrouche, Zoulika, Heliot, Laurent, McNally, James G

“…For gene regulation, some transcriptional activators bind periodically to promoters with either a fast (~1 minute) or a slow (~15 to 90 minutes) cycle. It is…”
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Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload by Kuipers, Marjorie A, Stasevich, Timothy J, Sasaki, Takayo, Wilson, Korey A, Hazelwood, Kristin L, McNally, James G, Davidson, Michael W, Gilbert, David M

“…The heterohexameric minichromosome maintenance protein complex (Mcm2-7) functions as the eukaryotic helicase during DNA replication. Mcm2-7 loads onto…”
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Hsp90 Inhibition Transiently Activates Src Kinase and Promotes Src-Dependent Akt and Erk Activation by Koga, Fumitaka, Xu, Wanping, Karpova, Tatiana S., McNally, James G., Baron, Roland, Neckers, Len

“…Hsp90 plays an essential role in maintaining stability and activity of its clients, including oncogenic signaling proteins that regulate key signal…”
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Towards an atlas of mammalian cell ultrastructure by cryo soft X-ray tomography by Müller, Waltraud G., Bernard Heymann, J., Nagashima, Kunio, Guttmann, Peter, Werner, Stephan, Rehbein, Stefan, Schneider, Gerd, McNally, James G.

“…We provide a catalog of 3D cryo soft X-ray tomography (cryo-SXT) images obtained from ∼6 to 12μm thick mouse adenocarcinoma cells. Included are multiple…”
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Assembly of the transcription machinery: ordered and stable, random and dynamic, or both? by Stasevich, Timothy J, McNally, James G

“…The assembly of the transcription machinery is a key step in gene activation, but even basic details of this process remain unclear. Here we discuss the…”
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Rapid Glucocorticoid Receptor Exchange at a Promoter Is Coupled to Transcription and Regulated by Chaperones and Proteasomes by Stavreva, Diana A., Müller, Waltraud G., Hager, Gordon L., Smith, Carolyn L., McNally, James G.

“…Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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A Reaction-Diffusion Model to Study RNA Motion by Quantitative Fluorescence Recovery after Photobleaching by Braga, José, McNally, James G., Carmo-Fonseca, Maria

“…Fluorescence recovery after photobleaching (FRAP) is a powerful technique to study molecular dynamics inside living cells. During the past years, several…”
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