Search Results - "Mcintosh, Lawrence P."

Arginine: Its pKa value revisited by Fitch, Carolyn A, Platzer, Gerald, Okon, Mark, Garcia-Moreno, Bertrand E, McIntosh, Lawrence P

“…Using complementary approaches of potentiometry and NMR spectroscopy, we have determined that the equilibrium acid dissociation constant (pKa value) of the…”
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Biophysical characterization of the ETV6 PNT domain polymerization interfaces by Gerak, Chloe A.N., Cho, Sophia Y., Kolesnikov, Maxim, Okon, Mark, Murphy, Michael E.P., Sessions, Richard B., Roberge, Michel, McIntosh, Lawrence P.

“…ETV6 is an E26 transformation specific family transcriptional repressor that self-associates by its PNT domain to facilitate cooperative DNA binding…”
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The Modular Structure of the Inner-Membrane Ring Component PrgK Facilitates Assembly of the Type III Secretion System Basal Body by Bergeron, Julien R.C., Worrall, Liam J., De, Soumya, Sgourakis, Nikolaos G., Cheung, Adrienne H., Lameignere, Emilie, Okon, Mark, Wasney, Gregory A., Baker, David, McIntosh, Lawrence P., Strynadka, Natalie C.J.

“…The type III secretion system (T3SS) is a large macromolecular assembly found at the surface of many pathogenic Gram-negative bacteria. Its role is to inject…”
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Synergy of aromatic residues and phosphoserines within the intrinsically disordered DNA-binding inhibitory elements of the Ets-1 transcription factor by Desjardins, Geneviève, Meeker, Charles A., Bhachech, Niraja, Currie, Simon L., Okon, Mark, Graves, Barbara J., McIntosh, Lawrence P.

“…The E26 transformation-specific (Ets-1) transcription factor is autoinhibited by a conformationally disordered serine-rich region (SRR) that transiently…”
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A Protein Export Pathway Involving Escherichia coli Porins by Prehna, Gerd, Zhang, Guijin, Gong, Xiandi, Duszyk, Marek, Okon, Mark, McIntosh, Lawrence P., Weiner, Joel H., Strynadka, Natalie C.J.

“…Escherichia coli export the protein YebF into the extracellular medium by a two-step process. However, as no general outer membrane protein secretion system…”
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Remeasuring HEWL pKa values by NMR spectroscopy: Methods, analysis, accuracy, and implications for theoretical pKa calculations by Webb, Helen, Tynan-Connolly, Barbara Mary, Lee, Gregory M., Farrell, Damien, O'Meara, Fergal, Søndergaard, Chresten R., Teilum, Kaare, Hewage, Chandralal, McIntosh, Lawrence P., Nielsen, Jens Erik

“…Site‐specific pKa values measured by NMR spectroscopy provide essential information on protein electrostatics, the pH‐dependence of protein structure, dynamics…”
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Discovery and characterization of small molecules targeting the DNA-binding ETS domain of ERG in prostate cancer by Butler, Miriam S, Roshan-Moniri, Mani, Hsing, Michael, Lau, Desmond, Kim, Ari, Yen, Paul, Mroczek, Marta, Nouri, Mannan, Lien, Scott, Axerio-Cilies, Peter, Dalal, Kush, Yau, Clement, Ghaidi, Fariba, Guo, Yubin, Yamazaki, Takeshi, Lawn, Sam, Gleave, Martin E, Gregory-Evans, Cheryl Y, McIntosh, Lawrence P, Cox, Michael E, Rennie, Paul S, Cherkasov, Artem

“…Genomic alterations involving translocations of the ETS-related gene ERG occur in approximately half of prostate cancer cases. These alterations result in…”
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Variable Control of Ets-1 DNA Binding by Multiple Phosphates in an Unstructured Region by Pufall, Miles A., Lee, Gregory M., Nelson, Mary L., Kang, Hyun-Seo, Velyvis, Algirdas, Kay, Lewis E., McIntosh, Lawrence P., Graves, Barbara J.

“…Cell signaling that culminates in posttranslational modifications directs protein activity. Here we report how multiple$Ca^{2+}-dependent$phosphorylation sites…”
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Putting a Stop to l,d-Transpeptidases by De, Soumya, McIntosh, Lawrence P.

“…In this issue of Structure, Lecoq et al. investigated the structural and dynamic basis for the unexpected inhibition of peptidoglycan-crosslinking…”
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pH-dependent random coil (1)H, (13)C, and (15)N chemical shifts of the ionizable amino acids: a guide for protein pK a measurements by Platzer, Gerald, Okon, Mark, McIntosh, Lawrence P

“…The pK a values and charge states of ionizable residues in polypeptides and proteins are frequently determined via NMR-monitored pH titrations. To aid the…”
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Ras signaling requires dynamic properties of Ets1 for phosphorylation-enhanced binding to coactivator CBP by Nelson, Mary L, Kang, Hyun-Seo, Lee, Gregory M, Blaszczak, Adam G, Lau, Desmond K.W, McIntosh, Lawrence P, Graves, Barbara J

“…Ras/MAPK signaling is often aberrantly activated in human cancers. The downstream effectors are transcription factors, including those encoded by the ETS gene…”
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Mutation of the Salt Bridge-forming Residues in the ETV6-SAM Domain Interface Blocks ETV6-NTRK3-induced Cellular Transformation by Cetinbas, Naniye, Huang-Hobbs, Helen, Tognon, Cristina, Leprivier, Gabriel, An, Jianghong, McKinney, Steven, Bowden, Mary, Chow, Connie, Gleave, Martin, McIntosh, Lawrence P., Sorensen, Poul H.

“…The ETV6-NTRK3 (EN) chimeric oncogene is expressed in diverse tumor types. EN is generated by a t(12;15) translocation, which fuses the N-terminal SAM (sterile…”
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The Crystal Structure of MexR from Pseudomonas aeruginosa in Complex with Its Antirepressor Armr by Wilke, Mark S., Heller, Markus, Creagh, A. Louise, Haynes, Charles A., McIntosh, Lawrence P., Poole, Keith, Strynadka, Natalie C. J.

“…The intrinsic antimicrobial resistance of the opportunistic human pathogen Pseudomonas aeruginosa is compounded in mutant strains that overexpress multidrug…”
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Structure, dynamics, and ionization equilibria of the tyrosine residues in Bacillus circulans xylanase by Baturin, Simon J., Okon, Mark, McIntosh, Lawrence P.

“…We have developed NMR spectroscopic methods to investigate the tyrosines within Bacillus circulans xylanase (BcX). Four slowly exchanging buried tyrosine…”
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A Secondary Xylan-binding Site Enhances the Catalytic Activity of a Single-domain Family 11 Glycoside Hydrolase by Ludwiczek, Martin L., Heller, Markus, Kantner, Terrence, McIntosh, Lawrence P.

“…Bacillus circulans xylanase (BcX) is a single-domain family 11 glycoside hydrolase. Using NMR-monitored titrations, we discovered that an inactive variant of…”
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A Multipronged Screening Approach Targeting Inhibition of ETV6 PNT Domain Polymerization by Gerak, Chloe A N, Zhang, Si Miao, Balgi, Aruna D, Sadowski, Ivan J, Sessions, Richard B, McIntosh, Lawrence P, Roberge, Michel

“…ETV6 is an ETS family transcriptional repressor for which head-to-tail polymerization of its PNT domain facilitates cooperative binding to DNA by its ETS…”
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Direct Demonstration of the Flexibility of the Glycosylated Proline-Threonine Linker in the Cellulomonas fimi Xylanase Cex through NMR Spectroscopic Analysis by Poon, David K.Y., Withers, Stephen G., McIntosh, Lawrence P.

“…The modular xylanase Cex (or CfXyn10A) from Cellulomonas fimi consists of an N-terminal catalytic domain and a C-terminal cellulose-binding domain, joined by a…”
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Titration_DB: Storage and analysis of NMR-monitored protein pH titration curves by Farrell, Damien, Miranda, Emanuel Sá, Webb, Helen, Georgi, Nikolaj, Crowley, Peter B., McIntosh, Lawrence P., Nielsen, Jens Erik

“…NMR‐monitored pH titration experiments are routinely used to measure site‐specific protein pKa values. Accurate experimental pKa values are essential in…”
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The Affinity of Ets-1 for DNA is Modulated by Phosphorylation Through Transient Interactions of an Unstructured Region by Lee, Gregory M., Pufall, Miles A., Meeker, Charles A., Kang, Hyun-Seo, Graves, Barbara J., McIntosh, Lawrence P.

“…Binding of the transcription factor Ets-1 to DNA is allosterically regulated by a serine-rich region (SRR) that modulates the dynamic character of the adjacent…”
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Structural Characterization of the Type-III Pilot-Secretin Complex from Shigella flexneri by Okon, Mark, Moraes, Trevor F., Lario, Paula I., Creagh, A. Louise, Haynes, Charles A., Strynadka, Natalie C.J., McIntosh, Lawrence P.

“…Assembly of the type-III secretion apparatus, which translocates proteins through both membranes of Gram-negative bacterial pathogens into host cells, requires…”
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