Search Results - "Mchaourab, H S"

Modeling conformational states of proteins with AlphaFold by Sala, D., Engelberger, F., Mchaourab, H.S., Meiler, J.

“…Many proteins exert their function by switching among different structures. Knowing the conformational ensembles affiliated with these states is critical to…”
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De novo mutation in the dopamine transporter gene associates dopamine dysfunction with autism spectrum disorder by Hamilton, P J, Campbell, N G, Sharma, S, Erreger, K, Herborg Hansen, F, Saunders, C, Belovich, A N, Sahai, M A, Cook, E H, Gether, U, Mchaourab, H S, Matthies, H J G, Sutcliffe, J S, Galli, A

“…De novo genetic variation is an important class of risk factors for autism spectrum disorder (ASD). Recently, whole-exome sequencing of ASD families has…”
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The Yeast Mitochondrial Citrate Transport Protein:  Determination of Secondary Structure and Solvent Accessibility of Transmembrane Domain IV Using Site-Directed Spin Labeling by Kaplan, Ronald S, Mayor, June A, Kotaria, Rusudan, Walters, D. Eric, Mchaourab, Hassane S

“…To explore the spatial organization and functional dynamics of the citrate transport protein (CTP), a nitroxide scan was carried out along 22 consecutive…”
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Subunit Exchange of Small Heat Shock Proteins. Analysis of Oligomer Formation of alpha A-Crystallin and Hsp27 by Fluorescence Resonance Energy Transfer and Site-Directed Truncations by Bova, M P, Mchaourab, H S, Han, Y, Fung, BKa

“…alpha A-Crystallin, a member of the small heat shock protein (sHsp) family, is a large multimeric protein composed of 30-40 identical subunits. Its quaternary…”
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Motion of Spin-Labeled Side Chains in T4 Lysozyme. Correlation with Protein Structure and Dynamics by Mchaourab, Hassane S, Lietzow, Michael A, Hideg, Kalman, Hubbell, Wayne L

“…Thirty single cysteine substitution mutants of T4 lysozyme have been prepared and spin-labeled with a sulfhydryl-specific nitroxide reagent in order to…”
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Small heat-shock protein structures reveal a continuum from symmetric to variable assemblies by Haley, Dana A., Bova, Michael P., Huang, Qing-Ling, Mchaourab, Hassane S., Stewart, Phoebe L.

“…The small heat-shock proteins (sHSPs) form a diverse family of proteins that are produced in all organisms. They function as chaperone-like proteins in that…”
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Watching proteins move using site-directed spin labeling by Hubbell, Wayne L, Mchaourab, Hassane S, Altenbach, Christian, Lietzow, Michael A

“…Site-directed spin labeling of proteins has proven to be a practical means for determining secondary structure and its orientation; surfaces of tertiary…”
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Drosophila melanogaster: a novel animal model for the behavioral characterization of autism-associated mutations in the dopamine transporter gene by Hamilton, P J, Campbell, N G, Sharma, S, Erreger, K, Hansen, F H, Saunders, C, Belovich, A N, Sahai, M A, Cook, E H, Gether, U, Mchaourab, H S, Matthies, H J G, Sutcliffe, J S, Galli, A

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Multiquantum EPR of the mixed valence copper site in nitrous oxide reductase by Mchaourab, H.S., Pfenninger, S., Antholine, W.E., Felix, C.C., Hyde, J.S., Kroneck, P.M.

“…This work demonstrates the use of multiquantum EPR to study the magnetic properties of copper complexes and copper proteins. Pure absorption spectra are…”
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Mechanism of Chaperone Function in Small Heat-shock Proteins: FLUORESCENCE STUDIES OF THE CONFORMATIONS OF T4 LYSOZYME BOUND TO alpha B-CRYSTALLIN by Sathish, HA, Stein, R A, Yang, G, Mchaourab, H S

“…To further develop the mechanistic understanding of small heat-shock protein (sHSP) chaperone activity, we investigate the nature of the intermediate states…”
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Aggregation state of spin-labeled cecropin AD in solution by Mchaourab, Hassane S, Hyde, James S, Feix, Jimmy B

“…A spin-labeled derivative of the ion channel peptide cecropin AD (Fink et al.,1988) was synthesized and used to investigate its aggregation state in water and…”
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Conformation of T4 Lysozyme in Solution. Hinge-Bending Motion and the Substrate-Induced Conformational Transition Studied by Site-Directed Spin Labeling by Mchaourab, Hassane S, Oh, Kyoung Joon, Fang, Celia J, Hubbell, Wayne L

“…T4 lysozyme and mutants thereof crystallize in different conformations that are related to each other by a bend about a hinge in the molecule. This observation…”
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Site-directed Spin Labeling Study of Subunit Interactions in the alpha -Crystallin Domain of Small Heat-shock Proteins: Comparison of the Oligomer Symmetry in alpha A-Crystallin, HSP 27, and HSP 16.3 by Berengian, A R, Parfenova, M, Mchaourab, H S

“…Site-directed spin labeling was used to investigate quaternary interactions along a conserved sequence in the alpha -crystallin domain of alpha A-crystallin,…”
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A Multifrequency Electron Spin Resonance Study of T4 Lysozyme Dynamics by Barnes, Jeff P., Liang, Zhichun, Mchaourab, Hassane S., Freed, Jack H., Hubbell, Wayne L.

“…Electron spin resonance (ESR) spectroscopy at 250 GHz and 9 GHz is utilized to study the dynamics and local structural ordering of a nitroxide-labeled enzyme,…”
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Motion of Spin-Labeled Side Chains in T4 Lysozyme:  Effect of Side Chain Structure by Mchaourab, Hassane S, Kálai, Tamás, Hideg, Kálmán, Hubbell, Wayne L

“…Previous studies have shown that the mobility of nitroxide side chains in a protein, inferred from the electron paramagnetic resonance (EPR) spectra, can be…”
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Determination of Protein Secondary Structure and Solvent Accessibility Using Site-Directed Fluorescence Labeling. Studies of T4 Lysozyme Using the Fluorescent Probe Monobromobimane by Mansoor, Steven E, Mchaourab, Hassane S, Farrens, David L

“…We report an investigation of how much protein structural information could be obtained using a site-directed fluorescence labeling (SDFL) strategy. In our…”
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Identification of Protein Folding Patterns Using Site-Directed Spin Labeling. Structural Characterization of a β-Sheet and Putative Substrate Binding Regions in the Conserved Domain of αA-Crystallin by Koteiche, Hanane A, Berengian, Anderee R, Mchaourab, Hassane S

“…The folding pattern of the segment of αA-crystallin encoded by exon 2 and containing putative substrate binding sites was explored using site-directed spin…”
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Alternating Access of the Putative Substrate-Binding Chamber in the ABC Transporter MsbA by Zou, Ping, Mchaourab, Hassane S.

“…MsbA is an ATP-binding cassette transporter from Escherichia coli that is involved in trafficking lipid A across the inner membrane. ATP-binding cassette…”
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Site-Directed Spin-Labeling Study of the Structure and Subunit Interactions along a Conserved Sequence in the α-Crystallin Domain of Heat-Shock Protein 27. Evidence of a Conserved Subunit Interface by Mchaourab, Hassane S, Berengian, Anderee R, Koteiche, Hanane A

“…Site-directed spin-labeling (SDSL) was used to investigate the secondary structure, solvent accessibility, and tertiary and quaternary interactions along the…”
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Binding and State of Aggregation of Spin-Labeled Cecropin AD in Phospholipid Bilayers: Effects of Surface Charge and Fatty Acyl Chain Length by Mchaourab, Hassane S, Hyde, James S, Feix, Jimmy B

“…The binding and state of aggregation of cecropin in large unilamellar vesicles of different surface potential and varying acyl chain length were examined using…”
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