Inversion and deletion of 16q22 defined by array CGH, FISH, and RT-PCR in a patient with AML

Acute myelomonocytic leukemia with eosinophilia is commonly associated with pericentric inversions of chromosome 16, involving the core binding factor beta gene ( CBFB ) on 16q22 and the myosin heavy chain gene ( MYH11 ) on 16p13. The inv(16)(p13q22) results in a fusion gene comprising the 5′CBFB ge...

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Published in:	Cancer genetics Vol. 204; no. 6; pp. 344 - 347
Main Authors:	Dawson, Angelika J, Bal, Shibani, McTavish, Barry, Tomiuk, Michelle, Schroedter, Ingo, Ahsanuddin, Arshad N, Seftel, Matthew D, Vallente, Rhea, Mai, Sabine, Cotter, Philip D, Hovanes, Karine, Gorre, Mercedes, Gunn, Shelly R
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-06-2011
Subjects:	aCGH Acute myeloid leukemia Adult Chromosome Deletion Chromosome Inversion Chromosomes, Human, Pair 16 Comparative Genomic Hybridization deletion 3′CBFB FISH Hematology, Oncology and Palliative Medicine Humans In Situ Hybridization, Fluorescence inv Leukemia, Myeloid, Acute - genetics Male Medical Education microarray Reverse Transcriptase Polymerase Chain Reaction inv FISH microarray deletion 3′CBFB aCGH
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Summary:	Acute myelomonocytic leukemia with eosinophilia is commonly associated with pericentric inversions of chromosome 16, involving the core binding factor beta gene ( CBFB ) on 16q22 and the myosin heavy chain gene ( MYH11 ) on 16p13. The inv(16)(p13q22) results in a fusion gene comprising the 5′CBFB gene and the 3′MYH11 gene on the short arm of chromosome 16. The fusion gene interferes with the normal transcription of the CBFA/CBFB heterodimer and disrupts myeloid differentiation. The inv(16) is associated with a good prognosis. The inv(16) with deletion of the 3′CBFB region of the gene is a very rare occurrence. Although the number of cases is small, inv(16) with a deleted 3′CBFB seems to be associated with a poorer prognosis than that generally associated with inv(16). Our patient was a 30-year-old man with newly diagnosed acute myeloid leukemia who was found to have a CBFB–MYH11 fusion by reverse transcriptase–polymerase chain reaction. The high blast count and lack of differentiation were not typical for this entity and suggested clonal progression. The initial karyotype by conventional cytogenetic analysis, in all metaphases examined, was 46,XY,del(7)(q32),del(16)(q22). Fluorescence in situ hybridization analysis with a dual-color, break-apart probe corresponding to the CBFB gene locus (Abbott, Des Plaines, IL) showed a derivative chromosome 16 resulting from an inversion of the CBFB gene with a deletion of the 3′CBFB probe region. Oligonucleotide array comparative genetic hybridization analysis was performed on this patient's diagnostic bone marrow DNA referenced to a normal male control DNA by using the DNA array Heme Profile (CombiMatrix Diagnostics, Irvine, CA) microarray. This analysis showed a 1.2 Mb loss of 16q22.1, which did not include loss of the 3′CBFB gene locus, but rather sequences distal to this locus. The DNA array Heme Profile results illustrate the importance of microarray in the correct identification of abnormalities that will affect prognosis.
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ISSN:	2210-7762 2210-7770
DOI:	10.1016/j.cancergen.2011.05.005