Search Results - "McKee, Lauren"
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Bacteroidetes bacteria in the soil: Glycan acquisition, enzyme secretion, and gliding motility
Published in Advances in applied microbiology (2020)“…The secretion of extracellular enzymes by soil microbes is rate-limiting in the recycling of biomass. Fungi and bacteria compete and collaborate for nutrients…”
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Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay
Published in Methods in molecular biology (Clifton, N.J.) (2017)“…Use of the 3,5-dinitrosalicylic acid reagent allows the simple and rapid quantification of reducing sugars. The method can be used for analysis of biological…”
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Growth of Chitinophaga pinensis on Plant Cell Wall Glycans and Characterisation of a Glycoside Hydrolase Family 27 β-l-Arabinopyranosidase Implicated in Arabinogalactan Utilisation
Published in PloS one (08-10-2015)“…The genome of the soil bacterium Chitinophaga pinensis encodes a diverse array of carbohydrate active enzymes, including nearly 200 representatives from over…”
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Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay
Published in Methods in molecular biology (Clifton, N.J.) (2023)“…Use of the 3,5-dinitrosalicylic acid reagent allows the simple, rapid quantification of reducing sugars. The method can be used for analysis of biological…”
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A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases
Published in The Journal of biological chemistry (01-01-2021)“…The gut microbiota plays a central role in human health by enzymatically degrading dietary fiber and concomitantly excreting short chain fatty acids that are…”
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Comparative analysis of sterol acquisition in the oomycetes Saprolegnia parasitica and Phytophthora infestans
Published in PloS one (02-02-2017)“…The oomycete class includes pathogens of animals and plants which are responsible for some of the most significant global losses in agriculture and…”
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Structure-property relationship of native-like lignin nanoparticles from softwood and hardwood
Published in Industrial crops and products (15-12-2023)“…Renewable resources such as wood are an important candidate towards the replacement of fossil-based materials with bio-based materials. Lignin, comprising up…”
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Polysaccharide degradation by the Bacteroidetes: mechanisms and nomenclature
Published in Environmental microbiology reports (01-10-2021)“…Summary The Bacteroidetes phylum is renowned for its ability to degrade a wide range of complex carbohydrates, a trait that has enabled its dominance in many…”
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Focused Metabolism of β-Glucans by the Soil Bacteroidetes Species Chitinophaga pinensis
Published in Applied and environmental microbiology (01-01-2019)“…The genome and natural habitat of suggest it has the ability to degrade a wide variety of carbohydrate-based biomass. Complementing our earlier investigations…”
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A discrete genetic locus confers xyloglucan metabolism in select human gut Bacteroidetes
Published in Nature (London) (27-02-2014)“…A genetic locus from the gut symbiont Bacteroides ovatus is identified and described that encodes a cohort of enzymes and carbohydrate-binding proteins…”
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Family 92 carbohydrate-binding modules specific for β-1,6-glucans increase the thermostability of a bacterial chitinase
Published in Biochimie (01-09-2023)“…In biomass-processing industries there is a need for enzymes that can withstand high temperatures. Extensive research efforts have been dedicated to finding…”
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Glycan processing in gut microbiomes
Published in Current opinion in microbiology (01-06-2022)“…[Display omitted] •Gut microbiomes and their enzymes enable processing of glycans consumed or secreted by the host.•Common mechanisms of glycan utilization are…”
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Germline β−1,3-glucan deposits are required for female gametogenesis in Arabidopsis thaliana
Published in Nature communications (12-07-2024)“…Correct regulation of intercellular communication is a fundamental requirement for cell differentiation. In Arabidopsis thaliana , the female germline…”
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Multiple enzymatic approaches to hydrolysis of fungal β‐glucans by the soil bacterium Chitinophaga pinensis
Published in The FEBS journal (01-06-2023)“…The genome of the soil Bacteroidota Chitinophaga pinensis encodes a large number of glycoside hydrolases (GHs) with noteworthy features and potentially novel…”
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Structural and biochemical analysis of family 92 carbohydrate-binding modules uncovers multivalent binding to β-glucans
Published in Nature communications (23-04-2024)“…Carbohydrate-binding modules (CBMs) are non-catalytic proteins found appended to carbohydrate-active enzymes. Soil and marine bacteria secrete such enzymes to…”
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Exploring the Potential for Fungal Antagonism and Cell Wall Attack by Bacillus subtilis natto
Published in Frontiers in microbiology (31-03-2020)“…To develop more ecologically sustainable agricultural practices requires that we reduce our reliance on synthetic chemical pesticides for crop protection. This…”
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The degree of acetylation affects the microbial degradability of mannans
Published in Polymer degradation and stability (01-11-2016)“…Hemicelluloses as major components of plant cell walls are acetylated to different extents. The biological functions of acetylation are not completely…”
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Human Gut Faecalibacterium prausnitzii Deploys a Highly Efficient Conserved System To Cross-Feed on β-Mannan-Derived Oligosaccharides
Published in mBio (29-06-2021)“…β-Mannans are hemicelluloses that are abundant in modern diets as components in seed endosperms and common additives in processed food. Currently, the…”
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Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β‐subunit of the heterotrimeric G‐protein
Published in The Plant journal : for cell and molecular biology (01-11-2017)“…Summary Arabidopsis heterotrimeric G‐protein complex modulates pathogen‐associated molecular pattern‐triggered immunity (PTI) and disease resistance responses…”
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The Impact of Steroidal Glycoalkaloids on the Physiology of Phytophthora infestans, the Causative Agent of Potato Late Blight
Published in Molecular plant-microbe interactions (01-07-2017)“…Steroidal glycoalkaloids (SGAs) are plant secondary metabolites known to be toxic to animals and humans and that have putative roles in defense against pests…”
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