Cloning and characterization of a gene encoding extracellular metalloprotease from Streptomyces lividans

Cloning and characterization of a gene encoding extracellular metalloprotease from Streptomyces lividans

The prt gene, encoding a protease (Prt) from Streptomyces lividans TK24, was cloned and sequenced. An S. lividans host with plasmid-borne prt secreted 200 micrograms/ml of a 22-kDa Prt into the culture medium. Prt is classified as a metalloprotease since its activity is significantly inhibited by 1,...

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Bibliographic Details
Published in:Gene Vol. 111; no. 1; p. 125
Main Authors: Lichenstein, H S, Busse, L A, Smith, G A, Narhi, L O, McGinley, M O, Rohde, M F, Katzowitz, J L, Zukowski, M M
Format: Journal Article
Language:English
Published: Netherlands 01-02-1992
Subjects:
Amino Acid Sequence
Bacterial Proteins
Base Sequence
Cloning, Molecular
DNA, Bacterial
Electrophoresis, Polyacrylamide Gel
Genes, Bacterial
Genomic Library
Hydrogen-Ion Concentration
Metalloendopeptidases - genetics
Metalloendopeptidases - metabolism
Molecular Sequence Data
Restriction Mapping
Sequence Alignment
Streptomyces - enzymology
Streptomyces - genetics
Temperature
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Description
Summary:The prt gene, encoding a protease (Prt) from Streptomyces lividans TK24, was cloned and sequenced. An S. lividans host with plasmid-borne prt secreted 200 micrograms/ml of a 22-kDa Prt into the culture medium. Prt is classified as a metalloprotease since its activity is significantly inhibited by 1,10-phenanthroline or EDTA. The region upstream from prt codes for an incomplete open reading frame (ORF) oriented opposite to prt. This ORF has a strong similarity to a gene family (lysR) whose members regulate the transcription of structural genes required for either biosynthesis or degradation.
ISSN:0378-1119
DOI:10.1016/0378-1119(92)90613-t