Search Results - "McDonough, Patrick M."

Specific prediction of clinical QT prolongation by kinetic image cytometry in human stem cell derived cardiomyocytes by Pfeiffer, Emily R., Vega, Raquel, McDonough, Patrick M., Price, Jeffrey H., Whittaker, Ross

“…A priority in the development and approval of new drugs is assessment of cardiovascular risk. Current methodologies for screening compounds (e.g. HERG testing)…”
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High throughput measurement of Ca2+ dynamics for drug risk assessment in human stem cell-derived cardiomyocytes by kinetic image cytometry by Cerignoli, Fabio, Charlot, David, Whittaker, Ross, Ingermanson, Randy, Gehalot, Piyush, Savchenko, Alex, Gallacher, David J., Towart, Rob, Price, Jeffrey H., McDonough, Patrick M., Mercola, Mark

“…Current methods to measure physiological properties of cardiomyocytes and predict fatal arrhythmias that can cause sudden death, such as Torsade de Pointes,…”
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Diabetes and the Accompanying Hyperglycemia Impairs Cardiomyocyte Calcium Cycling through Increased Nuclear O-GlcNAcylation by Clark, Raymond J., McDonough, Patrick M., Swanson, Eric, Trost, Susanne U., Suzuki, Misa, Fukuda, Minoru, Dillmann, Wolfgang H.

“…Diabetic cardiomyopathy is characterized by impaired cardiac contractility leading to poor myocardial performance. We investigated the role that the hexosamine…”
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Differential phosphorylation of perilipin 1A at the initiation of lipolysis revealed by novel monoclonal antibodies and high content analysis by McDonough, Patrick M, Maciejewski-Lenoir, Dominique, Hartig, Sean M, Hanna, Rita A, Whittaker, Ross, Heisel, Andrew, Nicoll, James B, Buehrer, Benjamin M, Christensen, Kurt, Mancini, Maureen G, Mancini, Michael A, Edwards, Dean P, Price, Jeffrey H

“…Lipolysis in adipocytes is regulated by phosphorylation of lipid droplet-associated proteins, including perilipin 1A and hormone-sensitive lipase (HSL)…”
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A Role for the p38 Mitogen-Activated Protein Kinase Pathway in Myocardial Cell Growth, Sarcomeric Organization, and Cardiac-Specific Gene Expression by Zechner, Dietmar, Thuerauf, Donna J., Hanford, Deanna S., McDonough, Patrick M., Glembotski, Christopher C.

“…Three hallmark features of the cardiac hypertrophic growth program are increases in cell size, sarcomeric organization, and the induction of certain…”
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High-content analysis and Kinetic Image Cytometry identify toxicity and epigenetic effects of HIV antiretrovirals on human iPSC-neurons and primary neural precursor cells by Smith, Alyson S., Ankam, Soneela, Farhy, Chen, Fiengo, Lorenzo, Basa, Ranor C.B., Gordon, Kara L., Martin, Charles T., Terskikh, Alexey V., Jordan-Sciutto, Kelly L., Price, Jeffrey H., McDonough, Patrick M.

“…Despite viral suppression due to combination antiretroviral therapy (cART), HIV-associated neurocognitive disorders (HAND) continue to affect half of people…”
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A recombinant antibody increases cardiac contractility by mimicking phospholamban phosphorylation by Meyer, Markus, Belke, Darrell D., Trost, Susanne U., Swanson, Eric, Dieterle, Thomas, Scott, Brian, Cary, Stephen P., Ho, Peter, Bluhm, Wolfgang F., McDonough, Patrick M., Silverman, Gregg J., Dillmann, Wolfgang H.

“…ABSTRACT Many cardiovascular disease states end in progressive heart failure. Changes in intracellular calcium handling, including a reduced activity of the…”
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Sorcin modulates mitochondrial Ca(2+) handling and reduces apoptosis in neonatal rat cardiac myocytes by Suarez, Jorge, McDonough, Patrick M, Scott, Brian T, Suarez-Ramirez, Angelica, Wang, Hong, Fricovsky, Eduardo S, Dillmann, Wolfgang H

“…Sorcin localizes in cellular membranes and has been demonstrated to modulate cytosolic Ca(2+) handling in cardiac myocytes. Sorcin also localizes in…”
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Deletion of the inducible 70-kDa heat shock protein genes in mice impairs cardiac contractile function and calcium handling associated with hypertrophy by KIM, Yun-Kyung, SUAREZ, Jorge, YING HU, MCDONOUGH, Patrick M, BOER, Christa, DIX, David J, DILLMANN, Wolfgang H

“…Hspa1a and Hspa1b genes encode stress-inducible 70-kDa heat shock proteins (Hsp70) that protect cells from insults such as ischemia. Mice with null mutations…”
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Identification of MicroRNAs that control lipid droplet formation and growth in hepatocytes via high-content screening by Whittaker, Ross, Loy, Patricia A, Sisman, Eugene, Suyama, Eigo, Aza-Blanc, Pedro, Ingermanson, Randall S, Price, Jeffrey H, McDonough, Patrick M

“…Hepatic lipid droplets (LDs) are associated with metabolic syndrome, type 2 diabetes, hepatitis C, and both alcoholic and nonalcoholic fatty liver disease…”
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Automated Methods for the Analysis of Skeletal Muscle Fiber Size and Metabolic Type by Kostrominova, Tatiana Y., Reiner, David S., Haas, Richard H., Ingermanson, Randall, McDonough, Patrick M.

“…It is of interest to quantify the size, shape, and metabolic subtype of skeletal muscle fibers in many areas of biomedical research. To do so, skeletal muscle…”
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Assay of Calcium Transients and Synapses in Rat Hippocampal Neurons by Kinetic Image Cytometry and High-Content Analysis: An In Vitro Model System for Postchemotherapy Cognitive Impairment by McDonough, Patrick M, Prigozhina, Natalie L, Basa, Ranor C B, Price, Jeffrey H

“…Postchemotherapy cognitive impairment (PCCI) is commonly exhibited by cancer patients treated with a variety of chemotherapeutic agents, including the…”
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Quantification of lipid droplets and associated proteins in cellular models of obesity via high-content/high-throughput microscopy and automated image analysis by McDonough, Patrick M, Agustin, Ramses M, Ingermanson, Randall S, Loy, Patricia A, Buehrer, Benjamin M, Nicoll, James B, Prigozhina, Natalie L, Mikic, Ivana, Price, Jeffrey H

“…Intracellular lipid droplets are associated with a myriad of afflictions including obesity, fatty liver disease, coronary artery disease, and infectious…”
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A dynamic pool of calcium in catecholamine storage vesicles. Exploration in living cells by a novel vesicle-targeted chromogranin A-aequorin chimeric photoprotein by Mahapatra, Nitish R, Mahata, Manjula, Hazra, Partha P, McDonough, Patrick M, O'Connor, Daniel T, Mahata, Sushil K

“…Chromaffin vesicles contain very high concentration of Ca2+ (approximately 20-40 mM total), compared with approximately 100 nM in the cytosol. Aequorin, a…”
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In vivo adenoviral transfer of sorcin reverses cardiac contractile abnormalities of diabetic cardiomyopathy by Suarez, Jorge, Belke, Darrell D, Gloss, Bernd, Dieterle, Thomas, McDonough, Patrick M, Kim, Yun-Kyung, Brunton, Laurence L, Dillmann, Wolfgang H

“…In many types of heart failure cardiac myocyte Ca(2+) handling is abnormal because of downregulation of key Ca(2+) - handling proteins like sarco(endo)plasmic…”
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Quantification of hormone sensitive lipase phosphorylation and colocalization with lipid droplets in murine 3T3L1 and human subcutaneous adipocytes via automated digital microscopy and high-content analysis by McDonough, Patrick M, Ingermanson, Randall S, Loy, Patricia A, Koon, Erick D, Whittaker, Ross, Laris, Casey A, Hilton, Jeffrey M, Nicoll, James B, Buehrer, Benjamin M, Price, Jeffrey H

“…Lipolysis in adipocytes is associated with phosphorylation of hormone sensitive lipase (HSL) and translocation of HSL to lipid droplets. In this study,…”
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The MKK6-p38 MAPK pathway prolongs the cardiac contractile calcium transient, downregulates SERCA2, and activates NF-AT by ANDREWS, Catherine, HO, Peter D, DILLMANN, Wolfgang H, GLEMBOTSKI, Christopher C, MCDONOUGH, Patrick M

“…Our goal was to determine if the MKK6-p38 MAPK pathway regulates cardiac intracellular calcium ([Ca(2+)](i)). We also tested if MKK6 might influence expression…”
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Effects of mutant and antisense RNA of phospholamban on SR Ca2+-ATPase activity and cardiac myocyte contractility by HUAPING HE, MEYER, M, MARTIN, J. L, MCDONOUGH, P. M, HO, P, XIAOJING LOU, LEW, W. Y. W, HILAL-DANDAN, R, DILLMANN, W. H

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The Raf-MEK-ERK Cascade Represents a Common Pathway for Alteration of Intracellular Calcium by Ras and Protein Kinase C in Cardiac Myocytes by Ho, Peter D., Zechner, Dietmar K., He, Huaping, Dillmann, Wolfgang H., Glembotski, Christopher C., McDonough, Patrick M.

“…Ras and protein kinase C (PKC), which regulate the Raf-MEK-ERK cascade, may participate in the development of cardiac hypertrophy, a condition characterized by…”
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Plasma membrane assays and three-compartment image cytometry for high content screening by Prigozhina, Natalie L, Zhong, Ling, Hunter, Edward A, Mikić, Ivana, Callaway, Scott, Roop, Dennis R, Mancini, Michael A, Zacharias, David A, Price, Jeffrey H, McDonough, Patrick M

“…High throughput image cytometers analyze individual cells in digital photomicrographs by first assigning pixels within each image to plasma membrane,…”
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