Search Results - "McClean, Megan"

Transcription factor localization dynamics and DNA binding drive distinct promoter interpretations by Sweeney, Kieran, McClean, Megan N.

“…Environmental information may be encoded in the temporal dynamics of transcription factor (TF) activation and subsequently decoded by gene promoters to enact…”
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Single-cell RNA sequencing reveals intrinsic and extrinsic regulatory heterogeneity in yeast responding to stress by Gasch, Audrey P, Yu, Feiqiao Brian, Hose, James, Escalante, Leah E, Place, Mike, Bacher, Rhonda, Kanbar, Jad, Ciobanu, Doina, Sandor, Laura, Grigoriev, Igor V, Kendziorski, Christina, Quake, Stephen R, McClean, Megan N

“…From bacteria to humans, individual cells within isogenic populations can show significant variation in stress tolerance, but the nature of this heterogeneity…”
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Signal processing by the HOG MAP kinase pathway by Hersen, Pascal, McClean, Megan N, Mahadevan, L, Ramanathan, Sharad

“…Signaling pathways relay information about changes in the external environment so that cells can respond appropriately. How much information a pathway can…”
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Strain‐dependent differences in coordination of yeast signalling networks by Scott, Taylor D., Xu, Ping, McClean, Megan N.

“…The yeast mitogen‐activated protein kinase pathways serve as a model system for understanding how network interactions affect the way in which cells coordinate…”
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Lustro: High-Throughput Optogenetic Experiments Enabled by Automation and a Yeast Optogenetic Toolkit by Harmer, Zachary P., McClean, Megan N.

“…Optogenetic systems use genetically encoded light-sensitive proteins to control cellular processes. This provides the potential to orthogonally control cells…”
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Engineered bacteria self-organize to sense pressure by Morales, Neydis Moreno, McClean, Megan N

“…Bacteria are engineered with pattern-generating circuits to produce self-organized materials that can function as pressure sensors…”
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Automated calibration of optoPlate LEDs to reduce light dose variation in optogenetic experiments by Grødem, Edvard Os, Sweeney, Kieran, McClean, Megan N

“…Optogenetic systems use light to precisely control and investigate cellular processes. Until recently, there had been few instruments available for applying…”
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Single Combination Procedure for Acne Scarring by McClean, Megan E., Taylor, Mark B.

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High-Throughput Optogenetics Experiments in Yeast Using the Automated Platform Lustro by Harmer, Zachary P, McClean, Megan N

“…Optogenetics offers precise control over cellular behavior by utilizing genetically encoded light-sensitive proteins. However, optimizing these systems to…”
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Suture Lifting: A Review of the Literature and Our Experiences by McClean, Megan E., Boen, Monica, Alhaddad, Marwan, Hoss, Elika, Kollipara, Ramya, Butterwick, Kimberly

“…BACKGROUNDIn the past 5 years, several absorbable sutures for use in minimally invasive suture lifts have appeared on the market. These newer sutures were…”
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Live-cell analysis of IMPDH protein levels during yeast colony growth provides insights into the regulation of GTP synthesis by Shand, Erica L, Sweeney, Kieran, Sundling, Kaitlin E, McClean, Megan N, Brow, David A

“…The purine nucleotides ATP and GTP are made from the common precursor inosine monophosphate (IMP). Maintaining the correct balance of these nucleotides for…”
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Correction to “Lustro: High-Throughput Optogenetic Experiments Enabled by Automation and a Yeast Optogenetic Toolkit” by Harmer, Zachary P., McClean, Megan N.

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Tuning interdomain conjugation to enable in situ population modification in yeasts by Stindt, Kevin R, McClean, Megan N

“…The ability to modify and control natural and engineered microbiomes is essential for biotechnology and biomedicine. Fungi are critical members of most…”
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Modeling single-cell phenotypes links yeast stress acclimation to transcriptional repression and pre-stress cellular states by Bergen, Andrew C, Kocik, Rachel A, Hose, James, McClean, Megan N, Gasch, Audrey P

“…Stress defense and cell growth are inversely related in bulk culture analyses; however, these studies miss substantial cell-to-cell heterogeneity, thus…”
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A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae by An‐adirekkun, Jidapas (My), Stewart, Cameron J., Geller, Stephanie H., Patel, Michael T., Melendez, Justin, Oakes, Benjamin L., Noyes, Marcus B., McClean, Megan N.

“…Optogenetic tools for controlling gene expression are ideal for tuning synthetic biological networks due to the exquisite spatiotemporal control available with…”
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Protocol to probe how promoters decode TF dynamics in Saccharomyces cerevisiae by combining optogenetic control with microscopy by Sweeney, Kieran, Luffey, Emily L., McClean, Megan N.

“…Gene promoters filter transcription factor (TF) localization dynamics and changes in the DNA binding affinity of TFs. Here, we present a protocol to probe how…”
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Cross-talk and decision making in MAP kinase pathways by Ramanathan, Sharad, McClean, Megan N, Mody, Areez, Broach, James R

“…Cells must respond specifically to different environmental stimuli in order to survive. The signal transduction pathways involved in sensing these stimuli…”
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Real-time optogenetic control of intracellular protein concentration in microbial cell cultures by Melendez, Justin, Patel, Michael, Oakes, Benjamin L, Xu, Ping, Morton, Patrick, McClean, Megan N

“…Perturbations in the concentration of a specific protein are often used to study and control biological networks. The ability to "dial-in" and programmatically…”
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Noise and interlocking signaling pathways promote distinct transcription factor dynamics in response to different stresses by Petrenko, Natalia, Chereji, Razvan V, McClean, Megan N, Morozov, Alexandre V, Broach, James R

“…All cells perceive and respond to environmental stresses through elaborate stress-sensing networks. Yeast cells sense stress through diverse signaling pathways…”
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Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae by McIsaac, R Scott, Silverman, Sanford J, McClean, Megan N, Gibney, Patrick A, Macinskas, Joanna, Hickman, Mark J, Petti, Allegra A, Botstein, David

“…We describe the development and characterization of a system that allows the rapid and specific induction of individual genes in the yeast Saccharomyces…”
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