Enhanced Expansion of Human Pluripotent Stem Cells and Somatic Cell Reprogramming Using Defined and Xeno-Free Culture Conditions

Enhanced Expansion of Human Pluripotent Stem Cells and Somatic Cell Reprogramming Using Defined and Xeno-Free Culture Conditions

Human embryonic stem cells and induced pluripotent stem cells (hPSC) have an unprecedented opportunity to revolutionize the fields of developmental biology as well as tissue engineering and regenerative medicine. However, their applications have been significantly limited by the lack of chemically d...

Full description

Saved in:
Bibliographic Details
Published in:Bioengineering (Basel) Vol. 10; no. 9; p. 999
Main Authors: Timilsina, Suraj, McCandliss, Kaitlyn Faith, Trivedi, Evan, Villa-Diaz, Luis G
Format: Journal Article
Language:English
Published: Basel MDPI AG 24-08-2023
MDPI
Subjects:
Abnormalities
Bioengineering
Cell adhesion
Cell culture
Chloride
Chromosome aberrations
Cold
Culture media
Developmental biology
Diagnostic reagents industry
Embryo cells
Embryonic stem cells
human embryonic stem cells
human pluripotent stem cells
Medical research
Microwave heating
Nitrogen
Physiological aspects
Plates
Pluripotency
PMEDSAH
Polymerization
Polymers
Preconditioning
Regenerative medicine
reprogramming
Scientific equipment and supplies industry
self-renewal
Somatic cells
Stem cell research
Stem cells
synthetic polymer grafting
Tissue culture
Tissue engineering
United States
United States > US
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human embryonic stem cells and induced pluripotent stem cells (hPSC) have an unprecedented opportunity to revolutionize the fields of developmental biology as well as tissue engineering and regenerative medicine. However, their applications have been significantly limited by the lack of chemically defined and xeno-free culture conditions. The demand for the high-quality and scaled-up production of cells for use in both research and clinical studies underscores the need to develop tools that will simplify the in vitro culture process while reducing the variables. Here, we describe a systematic study to identify the optimal conditions for the initial cell attachment of hPSC to tissue culture dishes grafted with polymers of N-(3-Sulfopropyl)-N-Methacryloxyethyl-N, N-Dimethylammoniun Betaine (PMEDSAH) in combination with chemically defined and xeno-free culture media. After testing multiple supplements and chemicals, we identified that pre-conditioning of PMEDSAH grafted plates with 10% human serum (HS) supported the initial cell attachment, which allowed for the long-term culture and maintenance of hPSC compared to cells cultured on Matrigel-coated plates. Using this culture condition, a 2.1-fold increase in the expansion of hPSC was observed without chromosomal abnormalities. Furthermore, this culture condition supported a higher reprogramming efficiency (0.37% vs. 0.22%; p < 0.0068) of somatic cells into induced pluripotent stem cells compared to the non-defined culture conditions. This defined and xeno-free hPSC culture condition may be used in obtaining the large populations of hPSC and patient-derived iPSC required for many applications in regenerative and translational medicine.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2306-5354
2306-5354
DOI:10.3390/bioengineering10090999