Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury

Vigna subterranea is widely consumed as a traditional staple food in Nigeria and some West African countries. The ethanolic seed extract of V. subterranea (EEVS) was investigated for its gastroprotective effects on aspirin plus pylorus ligation-induced gastric ulcerated rats using an in vivo assay....

Full description

Saved in:

Bibliographic Details
Published in:	Journal of integrative medicine Vol. 16; no. 5; pp. 342 - 349
Main Authors:	Balogun, Morufu Eyitayo, Besong, Elizabeth Enohnyaket, Obimma, Jacinta Nkechi, Mbamalu, Ogochukwu Sophia, Djobissie, Fankou Serges Athanase
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-09-2018
Subjects:	Animals Anti-oxidative Anti-Ulcer Agents - pharmacology Anti-Ulcer Agents - therapeutic use Antioxidants - pharmacology Antioxidants - therapeutic use Aspirin Aspirin plus pylorus ligation Edema Gastric Mucosa - drug effects Gastric Mucosa - metabolism Gastric Mucosa - pathology Gastric ulcers Gastrointestinal Agents - pharmacology Gastrointestinal Agents - therapeutic use Glutathione Peroxidase - metabolism Hydrogen-Ion Concentration Leukocytes Male Malondialdehyde - metabolism Mucus - metabolism Nuts Phytotherapy Plant Extracts - pharmacology Plant Extracts - therapeutic use Rats Rats, Wistar Severity of Illness Index Stomach Ulcer - chemically induced Stomach Ulcer - drug therapy Stomach Ulcer - metabolism Stomach Ulcer - prevention & control Superoxide Dismutase - metabolism Vigna Vigna subterranea Rats Aspirin plus pylorus ligation Vigna subterranea Anti-oxidative Gastric ulcers
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Vigna subterranea is widely consumed as a traditional staple food in Nigeria and some West African countries. The ethanolic seed extract of V. subterranea (EEVS) was investigated for its gastroprotective effects on aspirin plus pylorus ligation-induced gastric ulcerated rats using an in vivo assay. Gastric mucosal ulceration was induced experimentally in Groups 2 to 5 using aspirin plus pylorus ligation. Rats in Group 1 were orally pretreated with 3% Tween 80 only as normal control. Groups 2 to 5 were pretreated with 3% Tween 80 (ulcer group), 20 mg/kg of omeprazole (positive group), and 200 and 400 mg/kg of EEVS (experimental groups), respectively, once daily for 21 days before ulcer induction. Parameters including those for gastric secretions, ulcerated areas and gastric wall histology were assessed. Levels of superoxide dismutase (SOD), glutathione peroxidase (GPX), and malondialdehyde (MDA) in the gastric tissue homogenate were also determined. Pretreatment with EEVS significantly (P < 0.05) reduced the ulcer index, gastric volume and total acidity in rats with aspirin plus pylorus ligation-induced ulcer. The pH and mucus of gastric content increased significantly (P < 0.05) while the levels of SOD and GPX were observed to be elevated with a reduced amount of MDA. Significant severe gastric mucosal injury was exhibited in the ulcer group and EEVS or omeprazole offered significant (P < 0.05) protection against mucosal ulceration. Histologically, the gastric submucosal layer showed remarkable decrease in edema and leucocytes infiltration compared with ulcer group. The study suggests that EEVS offered a protective action against aspirin plus pylorus ligation-induced gastric ulcers in Wistar rats. The protective effect might be mediated via antisecretory, cytoprotective and antioxidative mechanisms.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2095-4964
DOI:	10.1016/j.joim.2018.07.004