Dermal Microvascular Endothelial Cells Express the 180-kDa Macrophage Mannose Receptor In Situ and In Vitro

Expression of the 180-kDa mannose receptor (MR) is mainly found on cells of the macrophage lineage. MR mediates the uptake of micro-organisms and host-derived glycoproteins. We demonstrate that endothelium of the human skin in situ and dermal microvascular endothelial cells (DMEC) in vitro expressed...

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Bibliographic Details
Published in:	The Journal of immunology (1950) Vol. 165; no. 10; pp. 5428 - 5434
Main Authors:	Groger, Marion, Holnthoner, Wolfgang, Maurer, Dieter, Lechleitner, Sonja, Wolff, Klaus, Mayr, Bettina Beate, Lubitz, Werner, Petzelbauer, Peter
Format:	Journal Article
Language:	English
Published:	United States Am Assoc Immnol 15-11-2000
Subjects:	Cell Membrane - chemistry Cell Membrane - immunology Cell Membrane - metabolism Cell Membrane - microbiology Cells, Cultured Dextrans - metabolism Endothelium, Vascular - chemistry Endothelium, Vascular - cytology Endothelium, Vascular - immunology Endothelium, Vascular - metabolism Escherichia coli - chemistry Escherichia coli - immunology Escherichia coli - metabolism Humans Immunophenotyping Lectins, C-Type Ligands Macrophages - chemistry Macrophages - immunology Macrophages - metabolism Macrophages - microbiology Mannans - metabolism mannose receptors Mannose-Binding Lectins Microcirculation - cytology Microcirculation - immunology Microcirculation - metabolism Molecular Weight Organelles - chemistry Organelles - immunology Organelles - metabolism phagolysosomes Receptors, Cell Surface - biosynthesis Skin - blood supply Skin - cytology Skin - immunology Skin - metabolism
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Summary:	Expression of the 180-kDa mannose receptor (MR) is mainly found on cells of the macrophage lineage. MR mediates the uptake of micro-organisms and host-derived glycoproteins. We demonstrate that endothelium of the human skin in situ and dermal microvascular endothelial cells (DMEC) in vitro expressed MR at both the protein and mRNA levels. In contrast, HUVEC were consistently negative for MR expression. DMEC internalized dextran as well as Escherichia coli by the way of MR into acidic phagosomes, only a few of which fused with CD63- and lysosomal-associated membrane glycoprotein-2-positive lysosomes. This contrasts with the situation in monocyte-derived dendritic cells, where almost all of the MR-Ag complexes reached CD63- and lysosomal-associated membrane glycoprotein-2-positive compartments, indicating differences in the phagolysosomal fusion rate between DMEC and dendritic cells. In conclusion, DMEC express functional MR, a finding that corroborates a role of skin endothelium in Ag capture/clearing.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1767 1550-6606
DOI:	10.4049/jimmunol.165.10.5428