In Situ Identification of Both IL-4 and IL-10 Cytokine-Receptor Interactions during Tissue Regeneration

In Situ Identification of Both IL-4 and IL-10 Cytokine-Receptor Interactions during Tissue Regeneration

Cytokines secreted by individual immune cells regulate tissue regeneration and allow communication between various cell types. Cytokines bind to cognate receptors and trigger the healing process. Determining the orchestration of cytokine interactions with their receptors on their cellular targets is...

Full description

Saved in:
Bibliographic Details
Published in:Cells (Basel, Switzerland) Vol. 12; no. 11; p. 1522
Main Authors: Nikovics, Krisztina, Favier, Anne-Laure, Rocher, Mathilde, Mayinga, Céline, Gomez, Johanna, Dufour-Gaume, Frédérique, Riccobono, Diane
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 31-05-2023
MDPI
Subjects:
Blood vessels
Cell interaction
Cell research
Cytokine receptors
Cytokines
Dendritic cells
Development and progression
Endothelial cells
Ethanol
Health aspects
IL-10
IL-4
Inflammation
interaction
Interleukin 10
Interleukin 4
Leukocytes
Lymphocytes
Macrophages
Musculoskeletal system
Physiological aspects
Protein interaction
Proteins
proximity ligation assay
receptor
regeneration
Regeneration (Biology)
Tissue engineering
Tumor necrosis factor-TNF
France
Germany
IL-4
interaction
receptor
proximity ligation assay
regeneration
IL-10
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cytokines secreted by individual immune cells regulate tissue regeneration and allow communication between various cell types. Cytokines bind to cognate receptors and trigger the healing process. Determining the orchestration of cytokine interactions with their receptors on their cellular targets is essential to fully understanding the process of inflammation and tissue regeneration. To this end, we have investigated the interactions of Interleukin-4 cytokine (IL-4)/Interleukin-4 cytokine receptor (IL-4R) and Interleukin-10 cytokine (IL-10)/Interleukin-10 cytokine receptor (IL-10R) using in situ Proximity Ligation Assays in a regenerative model of skin, muscle and lung tissues in the mini-pig. The pattern of protein-protein interactions was distinct for the two cytokines. IL-4 bound predominantly to receptors on macrophages and endothelial cells around the blood vessels while the target cells of IL-10 were mainly receptors on muscle cells. Our results show that in situ studies of cytokine-receptor interactions can unravel the fine details of the mechanism of action of cytokines.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2073-4409
2073-4409
DOI:10.3390/cells12111522