Search Results - "May, Andrew P."

DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks by van Overbeek, Megan, Capurso, Daniel, Carter, Matthew M., Thompson, Matthew S., Frias, Elizabeth, Russ, Carsten, Reece-Hoyes, John S., Nye, Christopher, Gradia, Scott, Vidal, Bastien, Zheng, Jiashun, Hoffman, Gregory R., Fuller, Christopher K., May, Andrew P.

“…The repair outcomes at site-specific DNA double-strand breaks (DSBs) generated by the RNA-guided DNA endonuclease Cas9 determine how gene function is altered…”
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Mapping the genomic landscape of CRISPR–Cas9 cleavage by Cameron, Peter, Fuller, Chris K, Donohoue, Paul D, Jones, Brittnee N, Thompson, Matthew S, Carter, Matthew M, Gradia, Scott, Vidal, Bastien, Garner, Elizabeth, Slorach, Euan M, Lau, Elaine, Banh, Lynda M, Lied, Alexandra M, Edwards, Leslie S, Settle, Alexander H, Capurso, Daniel, Llaca, Victor, Deschamps, Stéphane, Cigan, Mark, Young, Joshua K, May, Andrew P

“…SITE-Seq probes Cas9 cleavage sites in vitro and returns a comprehensive list of off-target sites at different Cas9–sgRNA concentrations. RNA-guided…”
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Noninvasive identification and monitoring of cancer mutations by targeted deep sequencing of plasma DNA by Forshew, Tim, Murtaza, Muhammed, Parkinson, Christine, Gale, Davina, Tsui, Dana W Y, Kaper, Fiona, Dawson, Sarah-Jane, Piskorz, Anna M, Jimenez-Linan, Mercedes, Bentley, David, Hadfield, James, May, Andrew P, Caldas, Carlos, Brenton, James D, Rosenfeld, Nitzan

“…Plasma of cancer patients contains cell-free tumor DNA that carries information on tumor mutations and tumor burden. Individual mutations have been probed…”
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Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality by Briner, Alexandra E., Donohoue, Paul D., Gomaa, Ahmed A., Selle, Kurt, Slorach, Euan M., Nye, Christopher H., Haurwitz, Rachel E., Beisel, Chase L., May, Andrew P., Barrangou, Rodolphe

“…The RNA-guided Cas9 endonuclease specifically targets and cleaves DNA in a sequence-dependent manner and has been widely used for programmable genome editing…”
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Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct Vectors by Rumachik, Neil G., Malaker, Stacy A., Poweleit, Nicole, Maynard, Lucy H., Adams, Christopher M., Leib, Ryan D., Cirolia, Giana, Thomas, Dennis, Stamnes, Susan, Holt, Kathleen, Sinn, Patrick, May, Andrew P., Paulk, Nicole K.

“…Different approaches are used in the production of recombinant adeno-associated virus (rAAV). The two leading approaches are transiently transfected human…”
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Clinical and pathological impact of VHL, PBRM1, BAP1, SETD2, KDM6A, and JARID1c in clear cell renal cell carcinoma by Gossage, Lucy, Murtaza, Muhammed, Slatter, Andrew F., Lichtenstein, Conrad P., Warren, Anne, Haynes, Beverley, Marass, Francesco, Roberts, Ian, Shanahan, Susan J., Claas, Andreas, Dunham, Andrew, May, Andrew P., Rosenfeld, Nitzan, Forshew, Tim, Eisen, Tim

“…VHL is mutated in the majority of patients with clear cell renal cell carcinoma (ccRCC), with conflicting clinical relevance. Recent studies have identified…”
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A functional map of HIV-host interactions in primary human T cells by Hiatt, Joseph, Hultquist, Judd F., McGregor, Michael J., Bouhaddou, Mehdi, Leenay, Ryan T., Simons, Lacy M., Young, Janet M., Haas, Paige, Roth, Theodore L., Tobin, Victoria, Wojcechowskyj, Jason A., Woo, Jonathan M., Rathore, Ujjwal, Cavero, Devin A., Shifrut, Eric, Nguyen, Thong T., Haas, Kelsey M., Malik, Harmit S., Doudna, Jennifer A., May, Andrew P., Marson, Alexander, Krogan, Nevan J.

“…Human Immunodeficiency Virus (HIV) relies on host molecular machinery for replication. Systematic attempts to genetically or biochemically define these host…”
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Unraveling the potential of CRISPR-Cas9 for gene therapy by Barrangou, Rodolphe, May, Andrew P

“…The molecular machinery from the prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)-Cas immune system has broadly been repurposed…”
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Structural Basis for the Assembly and Functions of a Viral Polymer that Inactivates Multiple Tumor Suppressors by Ou, Horng D, Kwiatkowski, Witek, Deerinck, Thomas J, Noske, Andrew, Blain, Katie Y, Land, Hannah S, Soria, Conrado, Powers, Colin J, May, Andrew P, Shu, Xiaokun, Tsien, Roger Y, Fitzpatrick, James A.J, Long, Jeff A, Ellisman, Mark H, Choe, Senyon, O'Shea, Clodagh C

“…Evolution of minimal DNA tumor virus' genomes has selected for small viral oncoproteins that hijack critical cellular protein interaction networks. The…”
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Advances in Industrial Biotechnology Using CRISPR-Cas Systems by Donohoue, Paul D., Barrangou, Rodolphe, May, Andrew P.

“…The term ‘clustered regularly interspaced short palindromic repeats’ (CRISPR) has recently become synonymous with the genome-editing revolution. The RNA-guided…”
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Macro-to-micro structural proteomics: native source proteins for high-throughput crystallization by Totir, Monica, Echols, Nathaniel, Nanao, Max, Gee, Christine L, Moskaleva, Alisa, Gradia, Scott, Iavarone, Anthony T, Berger, James M, May, Andrew P, Zubieta, Chloe, Alber, Tom

“…Structural biology and structural genomics projects routinely rely on recombinantly expressed proteins, but many proteins and complexes are difficult to obtain…”
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Conformational changes of the multifunction p97 AAA ATPase during its ATPase cycle by Wilson-Kubalek, Elizabeth M, Rouiller, Isabelle, DeLaBarre, Byron, May, Andrew P, Weis, William I, Brunger, Axel T, Milligan, Ronald A

“…p97 (also called VCP), a member of the AAA ATPase family, is involved in several cellular processes, including membrane fusion and extraction of proteins from…”
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Single-cell RNA-seq reveals dynamic paracrine control of cellular variation by Shalek, Alex K., Satija, Rahul, Shuga, Joe, Trombetta, John J., Gennert, Dave, Lu, Diana, Chen, Peilin, Gertner, Rona S., Gaublomme, Jellert T., Yosef, Nir, Schwartz, Schraga, Fowler, Brian, Weaver, Suzanne, Wang, Jing, Wang, Xiaohui, Ding, Ruihua, Raychowdhury, Raktima, Friedman, Nir, Hacohen, Nir, Park, Hongkun, May, Andrew P., Regev, Aviv

“…High-throughput single-cell transcriptomics offers an unbiased approach for understanding the extent, basis and function of gene expression variation between…”
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Conformational Changes of p97 during Nucleotide Hydrolysis Determined by Small-Angle X-Ray Scattering by Davies, Jason M., Tsuruta, Hirotsugu, May, Andrew P., Weis, William I.

“…Valosin-containing protein (VCP)/p97 is an AAA family ATPase that has been implicated in the removal of misfolded proteins from the endoplasmic reticulum and…”
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Reprogramming human T cell function and specificity with non-viral genome targeting by Roth, Theodore L., Puig-Saus, Cristina, Yu, Ruby, Shifrut, Eric, Carnevale, Julia, Li, P. Jonathan, Hiatt, Joseph, Saco, Justin, Krystofinski, Paige, Li, Han, Tobin, Victoria, Nguyen, David N., Lee, Michael R., Putnam, Amy L., Ferris, Andrea L., Chen, Jeff W., Schickel, Jean-Nicolas, Pellerin, Laurence, Carmody, David, Alkorta-Aranburu, Gorka, del Gaudio, Daniela, Matsumoto, Hiroyuki, Morell, Montse, Mao, Ying, Cho, Min, Quadros, Rolen M., Gurumurthy, Channabasavaiah B., Smith, Baz, Haugwitz, Michael, Hughes, Stephen H., Weissman, Jonathan S., Schumann, Kathrin, Esensten, Jonathan H., May, Andrew P., Ashworth, Alan, Kupfer, Gary M., Greeley, Siri Atma W., Bacchetta, Rosa, Meffre, Eric, Roncarolo, Maria Grazia, Romberg, Neil, Herold, Kevan C., Ribas, Antoni, Leonetti, Manuel D., Marson, Alexander

“…Decades of work have aimed to genetically reprogram T cells for therapeutic purposes 1 , 2 using recombinant viral vectors, which do not target transgenes to…”
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A large CRISPR-induced bystander mutation causes immune dysregulation by Simeonov, Dimitre R., Brandt, Alexander J., Chan, Alice Y., Cortez, Jessica T., Li, Zhongmei, Woo, Jonathan M., Lee, Youjin, Carvalho, Claudia M. B., Indart, Alyssa C., Roth, Theodore L., Zou, James, May, Andrew P., Lupski, James R., Anderson, Mark S., Buaas, F. William, Rokhsar, Daniel S., Marson, Alexander

“…A persistent concern with CRISPR-Cas9 gene editing has been the potential to generate mutations at off-target genomic sites. While CRISPR-engineering mice to…”
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Structure and Mechanism of ArnA: Conformational Change Implies Ordered Dehydrogenase Mechanism in Key Enzyme for Polymyxin Resistance by Gatzeva-Topalova, Petia Z., May, Andrew P., Sousa, Marcelo C.

“…The modification of lipid A with 4-amino-4-deoxy-L-arabinose (Ara4N) allows gram-negative bacteria to resist the antimicrobial activity of cationic…”
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Large dataset enables prediction of repair after CRISPR–Cas9 editing in primary T cells by Leenay, Ryan T., Aghazadeh, Amirali, Hiatt, Joseph, Tse, David, Roth, Theodore L., Apathy, Ryan, Shifrut, Eric, Hultquist, Judd F., Krogan, Nevan, Wu, Zhenqin, Cirolia, Giana, Canaj, Hera, Leonetti, Manuel D., Marson, Alexander, May, Andrew P., Zou, James

“…Understanding of repair outcomes after Cas9-induced DNA cleavage is still limited, especially in primary human cells. We sequence repair outcomes at 1,656…”
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Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex by Pollen, Alex A, Nowakowski, Tomasz J, Shuga, Joe, Wang, Xiaohui, Leyrat, Anne A, Lui, Jan H, Li, Nianzhen, Szpankowski, Lukasz, Fowler, Brian, Chen, Peilin, Ramalingam, Naveen, Sun, Gang, Thu, Myo, Norris, Michael, Lebofsky, Ronald, Toppani, Dominique, Kemp, Darnell W, Wong, Michael, Clerkson, Barry, Jones, Brittnee N, Wu, Shiquan, Knutsson, Lawrence, Alvarado, Beatriz, Wang, Jing, Weaver, Lesley S, May, Andrew P, Jones, Robert C, Unger, Marc A, Kriegstein, Arnold R, West, Jay A A

“…Pollen et al. show that low-coverage RNA sequencing of single cells is a powerful approach for characterizing heterogeneous cell populations. Large-scale…”
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Nucleic acid cleavage with a hyperthermophilic Cas9 from an uncultured Ignavibacterium by Schmidt, Stephanie Tzouanas, Yu, Feiqiao Brian, Blainey, Paul C., May, Andrew P., Quake, Stephen R.

“…Clustered regularly interspaced short palindromic repeats (CRISPR)- associated 9 (Cas9) systems have been effectively harnessed to engineer the genomes of…”
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