Search Results - "Mauro, Vincent P."

A critical analysis of codon optimization in human therapeutics by Mauro, Vincent P, Chappell, Stephen A

“…Highlights • Codon optimization is a gene engineering approach to increase protein production. • Assumptions underlying codon optimization approaches may be…”
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Base pairing between hepatitis C virus RNA and 18S rRNA is required for IRES-dependent translation initiation in vivo by Matsuda, Daiki, Mauro, Vincent P.

“…Significance Translation of hepatitis C virus (HCV) RNA is facilitated by an internal ribosome entry site (IRES) that binds small (40S) ribosomal subunits via…”
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Translation regulation by ribosomes: Increased complexity and expanded scope by Mauro, Vincent P., Matsuda, Daiki

“…The primary function of ribosomes is to decode mRNAs into polypeptide chains; however, this description is overly simplistic. Accumulating evidence shows that…”
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The Ribosome Filter Redux by Mauro, Vincent P., Edelman, Gerald M.

“…The ribosome filter hypothesis postulates that ribosomes are not simply translation machines but also function as regulatory elements that differentially…”
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Codon Optimization in the Production of Recombinant Biotherapeutics: Potential Risks and Considerations by Mauro, Vincent P.

“…Biotherapeutics are increasingly becoming the mainstay in the treatment of a variety of human conditions, particularly in oncology and hematology. The…”
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The Ribosome Filter Hypothesis by Mauro, Vincent P., Edelman, Gerald M.

“…A variety of posttranscriptional mechanisms affects the processing, subcellular localization, and translation of messenger RNAs (mRNAs). Translational control…”
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Determinants of initiation codon selection during translation in mammalian cells by Matsuda, Daiki, Mauro, Vincent P

“…Factors affecting translation of mRNA contribute to the complexity of eukaryotic proteomes. In some cases, translation of a particular mRNA can generate…”
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Antisense Masking Reveals Contributions of mRNA-rRNA Base Pairing to Translation of Gtx and FGF2 mRNAs by Panopoulos, Panagiotis, Mauro, Vincent P.

“…We previously showed that a 9-nucleotide sequence from the 5′ leader of the Gtx homeodomain mRNA facilitates translation initiation by base pairing to 18S…”
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Ribosomal Tethering and Clustering as Mechanisms for Translation Initiation by Chappell, Stephen A., Edelman, Gerald M., Mauro, Vincent P.

“…Eukaryotic mRNAs often recruit ribosomal subunits some distance upstream of the initiation codon; however, the mechanisms by which they reach the initiation…”
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A 9-nt Segment of a Cellular mRNA Can Function as an Internal Ribosome Entry Site (IRES) and When Present in Linked Multiple Copies Greatly Enhances IRES Activity by Chappell, Stephen A., Edelman, Gerald M., Mauro, Vincent P.

“…This study addresses the properties of a newly identified internal ribosome entry site (IRES) contained within the mRNA of the homeodomain protein Gtx…”
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Ribosomal Shunting Mediated by a Translational Enhancer Element That Base Pairs to 18S rRNA by Chappell, Stephen A., Dresios, John, Edelman, Gerald M., Mauro, Vincent P.

“…In eukaryotes, 40S ribosomal subunits move from their recruitment site on the mRNA to the initiation codon by an as yet poorly understood process. One…”
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Differential Utilization of Upstream AUGs in the β-Secretase mRNA Suggests That a Shunting Mechanism Regulates Translation by Rogers, George W., Edelman, Gerald M., Mauro, Vincent P.

“…β-Secretase [also known as the β-site amyloid precursor protein-cleaving enzyme 1 (BACE1)] is an enzyme involved in the production of Aβ-amyloid plaques in the…”
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Internal Initiation of Translation of Five Dendritically Localized Neuronal mRNAs by Pinkstaff, Jason K., Chappell, Stephen A., Mauro, Vincent P., Edelman, Gerald M., Krushel, Les A.

“…In neurons, translation of dendritically localized mRNAs is thought to play a role in affecting synaptic efficacy. Inasmuch as components of the translation…”
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The Internal Ribosome Entry Site (IRES) Contained within the RNA-binding Motif Protein 3 (Rbm3) mRNA Is Composed of Functionally Distinct Elements by Chappell, Stephen A., Mauro, Vincent P.

“…Although the internal ribosome entry sites (IRESes) of viral mRNAs are highly structured and comprise several hundred nucleotides, there is a variety of…”
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Considerations in the Use of Codon Optimization for Recombinant Protein Expression by Mauro, Vincent P, Chappell, Stephen A

“…Codon optimization is a gene engineering approach that is commonly used for enhancing recombinant protein expression. This approach is possible because (1)…”
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Reconciling contradictory reports regarding translation of BACE1 mRNA: initiation mechanism is altered by different expression systems by Koh, Dora C, Mauro, Vincent P

“…We previously showed that translation from the rat BACE1 5' leader is cap-dependent and that four AUG codons (AUG1-4) in the 5' leader were bypassed, partially…”
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Biochemical and Functional Analysis of a 9-nt RNA Sequence That Affects Translation Efficiency in Eukaryotic Cells by Chappell, Stephen A., Edelman, Gerald M., Mauro, Vincent P.

“…We previously identified an internal ribosome entry site (IRES) within the 5′ leader of the mRNA encoding the Gtx homeodomain protein and showed that shorter…”
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Transcript Leader Regions of two Saccharomyces cerevisiae mRNAs Contain Internal Ribosome Entry Sites That Function in Living Cells by Zhou, Wei, Edelman, Gerald M., Mauro, Vincent P.

“…In higher eukaryotes, translation of some mRNAs occurs by internal initiation. It is not known, however, whether this mechanism is used to initiate the…”
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Glucocorticoid Receptor Pathways are Involved in the Inhibition of Astrocyte Proliferation by Crossin, Kathryn L., Tai, Ming-Hong, Krushel, Leslie A., Mauro, Vincent P., Edelman, Gerald M.

“…In earlier studies, the neural cell adhesion molecule, N-CAM, was found to inhibit the proliferation of rat astrocytes both in vitro and in vivo. To identify…”
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Identification of Two Short Internal Ribosome Entry Sites Selected from Libraries of Random Oligonucleotides by Owens, Geoffrey C., Chappell, Stephen A., Mauro, Vincent P., Edelman, Gerald M.

“…Sequences that control translation of mRNA may play critical roles in regulating protein levels. One such element is the internal ribosome entry site (IRES)…”
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