Secretion of matrix metalloproteinases 2 and 9 and tissue inhibitor of metalloproteinases into follicular fluid during follicle development in equine ovaries

Extensive tissue remodelling is required in equine ovaries for follicle growth and development and also migration of the follicle to the ovulatory fossa, where ovulation occurs. The mechanisms for these processes are largely unexplored. Matrix metalloproteinases (MMPs) and their endogenous tissue in...

Full description

Saved in:

Bibliographic Details
Published in:	Reproduction (Cambridge, England) Vol. 121; no. 4; pp. 553 - 560
Main Authors:	Riley, SC, Gibson, AH, Leask, R, Mauchline, DJ, Pedersen, HG, Watson, ED
Format:	Journal Article
Language:	English
Published:	Colchester Society for Reproduction and Fertility 01-04-2001 Portland
Subjects:	Animals Biological and medical sciences Female Follicular Fluid - chemistry Follicular Fluid - enzymology Fundamental and applied biological sciences. Psychology Glycosylation Horses - physiology Immunohistochemistry Mammalian female genital system Matrix Metalloproteinase 2 - analysis Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 9 - analysis Matrix Metalloproteinase 9 - metabolism Morphology. Physiology Ovarian Follicle - chemistry Ovarian Follicle - physiology Ovary - chemistry Tissue Inhibitor of Metalloproteinase-1 - analysis Tissue Inhibitor of Metalloproteinase-1 - metabolism Tissue Inhibitor of Metalloproteinase-2 - analysis Tissue Inhibitor of Metalloproteinase-2 - metabolism Tissue Inhibitor of Metalloproteinase-3 - analysis Tissue Inhibitor of Metalloproteinase-3 - metabolism Tissue Inhibitor of Metalloproteinase-4 Tissue Inhibitor of Metalloproteinases - analysis Tissue Inhibitor of Metalloproteinases - metabolism Vertebrates: reproduction Enzyme Secretion Folliculogenesis Enzyme inhibitor Metalloendopeptidases EC 3.4.24.24 EC 3.4.24.35 Gelatinase B Gelatinase A Ovary Peptidases Vertebrata Mammalia Female genital system Horse Development Hydrolases Perissodactyla Ovarian follicle Ungulata
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Extensive tissue remodelling is required in equine ovaries for follicle growth and development and also migration of the follicle to the ovulatory fossa, where ovulation occurs. The mechanisms for these processes are largely unexplored. Matrix metalloproteinases (MMPs) and their endogenous tissue inhibitors (TIMPs) are important for control of breakdown of extracellular matrix during tissue remodelling. The aims of this study were to determine the pattern and sites of secretion of the gelatinases MMP-2 and -9 and TIMPs into follicular fluid during follicle development in mare ovaries. The predominant gelatinase detected in follicular fluid was MMP-2, which was present in similar amounts throughout follicular development, as demonstrated by zymography. MMP-9 was also present in follicular fluid and secretion increased significantly (P < 0.05) with development of follicles from < 10 mm to 11-20 mm in diameter. Follicular fluid also contained TIMP-1, TIMP-2, unglycosylated and glycosylated TIMP-3, and TIMP-4, as shown by reverse zymography. The abundance of TIMPs remained largely unchanged during follicle development. MMP-2 and -9 were localized by immunohistochemistry to stromal cells and granulosa and theca cells. TIMP-1, -2, -3 and -4 were present in granulosa and theca cells of the follicle and in stromal cells and also associated with extracellular matrix of the ovarian stromal tissue. The MMPs and TIMPs are likely to be involved in the regulation of the breakdown of extracellular matrix during tissue remodelling for follicle development and migration to the ovulation fossa in mares.
ISSN:	1470-1626 1741-7899
DOI:	10.1530/rep.0.1210553