Four putative subtypes of human parvovirus B19 based on amino acid polymorphism in the C-terminal region of non-structural protein

The nucleotide sequence of 10 isolates of human parvovirus B19 (B19) were determined and compared throughout 96.3% of the open reading frames (4145 nucleotides from nt. 509–4653). In the 4145 nucleotides, 122 mutation sites were found, of which 24 were accompanied by amino acid displacement. Further...

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Bibliographic Details
Published in:	Journal of medical virology Vol. 62; no. 1; pp. 60 - 69
Main Authors:	Fukada, Kenji, Matumoto, Kouji, Takakura, Fumihiro, Yamaki, Masaharu, Sato, Hiroyuki, Okochi, Kazuo, Maeda, Yoshiaki
Format:	Journal Article
Language:	English
Published:	New York John Wiley & Sons, Inc 01-09-2000 Wiley-Liss
Subjects:	Amino Acid Sequence Amino Acid Substitution Base Sequence Biological and medical sciences distribution DNA Mutational Analysis DNA, Viral - blood Fundamental and applied biological sciences. Psychology Genetics Genotype Human parvovirus B19 Humans Microbiology non-structural protein Open Reading Frames Parvovirus B19, Human - classification Parvovirus B19, Human - genetics Parvovirus B19, Human - isolation & purification Polymerase Chain Reaction Polymorphism, Genetic Polymorphism, Single-Stranded Conformational subtype Viral Nonstructural Proteins - genetics Virology Virus Typing Genetic variability Nucleotide sequence Parvoviridae Genotype Erythrovirus Subtype Virus B19 Aminoacid sequence Parvovirinae Polymorphism
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Summary:	The nucleotide sequence of 10 isolates of human parvovirus B19 (B19) were determined and compared throughout 96.3% of the open reading frames (4145 nucleotides from nt. 509–4653). In the 4145 nucleotides, 122 mutation sites were found, of which 24 were accompanied by amino acid displacement. Furthermore, the polymorphism of the amino acids was seen in about 110 bases near the carboxy terminal of the non‐structural protein, ranging from nt. 2011 to 2123, where four amino acid mutation points were found to exist. Based on the amino acid polymorphism of these four mutation sites in this area, 10 isolates of the B19 parvovirus could be divided into 4 subtypes (subtypes A, B, C, and D). The frequency of isolation of the subtypes depended on the time and location of collection of the B19 viremic blood specimens. J. Med. Virol. 62:60–69, 2000. © 2000 Wiley‐Liss, Inc.
Bibliography:	istex:E17B8770E62D2D94F5AF3F1C33CF008448511747 ark:/67375/WNG-MHHJSW84-8 ArticleID:JMV10 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0146-6615 1096-9071
DOI:	10.1002/1096-9071(200009)62:1<60::AID-JMV10>3.0.CO;2-X