Search Results - "Matulef, Kimberly"

Instantaneous ion configurations in the K⁺ ion channel selectivity filter revealed by 2D IR spectroscopy by Kratochvil, Huong T., Carr, Joshua K., Matulef, Kimberly, Annen, Alvin W., Li, Hui, Maj, Michal, Ostmeyer, Jared, Serrano, Arnaldo L., Raghuraman, H., Moran, Sean D., Skinner, J. L., Perozo, Eduardo, Roux, Benoît, Valiyaveetil, Francis I., Zanni, Martin T.

“…Potassium channels are responsible for the selective permeation of K⁺ ions across cell membranes. K⁺ ions permeate in single file through the selectivity…”
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Probing the Effects of Gating on the Ion Occupancy of the K+ Channel Selectivity Filter Using Two-Dimensional Infrared Spectroscopy by Kratochvil, Huong T, Maj, Michał, Matulef, Kimberly, Annen, Alvin W, Ostmeyer, Jared, Perozo, Eduardo, Roux, Benoît, Valiyaveetil, Francis I, Zanni, Martin T

“…The interplay between the intracellular gate and the selectivity filter underlies the structural basis for gating in potassium ion channels. Using a…”
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Ion-binding properties of a K⁺ channel selectivity filter in different conformations by Liu, Shian, Focke, Paul J., Matulef, Kimberly, Bian, Xuelin, Moënne-Loccoz, Pierre, Valiyaveetil, Francis I., Lockless, Steve W.

“…K⁺ channels are membrane proteins that selectively conduct K⁺ ions across lipid bilayers. Many voltage-gated K⁺ (KV) channels contain two gates, one at the…”
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A facile approach for the in vitro assembly of multimeric membrane transport proteins by Riederer, Erika A, Focke, Paul J, Georgieva, Elka R, Akyuz, Nurunisa, Matulef, Kimberly, Borbat, Peter P, Freed, Jack H, Blanchard, Scott C, Boudker, Olga, Valiyaveetil, Francis I

“…Membrane proteins such as ion channels and transporters are frequently homomeric. The homomeric nature raises important questions regarding coupling between…”
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Semisynthetic K⁺ channels show that the constricted conformation of the selectivity filter is not the C-type inactivated state by Devaraneni, Prasanna K., Komarov, Alexander G., Costantino, Corey A., Devereaux, Jordan J., Matulef, Kimberly, Valiyaveetil, Francis I.

“…C-type inactivation of K ⁺ channels plays a key role in modulating cellular excitability. During C-type inactivation, the selectivity filter of a K ⁺ channel…”
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Main-chain mutagenesis reveals intrahelical coupling in an ion channel voltage-sensor by Infield, Daniel T., Matulef, Kimberly, Galpin, Jason D., Lam, Kin, Tajkhorshid, Emad, Ahern, Christopher A., Valiyaveetil, Francis I.

“…Membrane proteins are universal signal decoders. The helical transmembrane segments of these proteins play central roles in sensory transduction, yet the…”
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CYCLIC NUCLEOTIDE-GATED ION CHANNELS by Matulef, Kimberly, Zagotta, William N

“…Cyclic nucleotide-gated (CNG) ion channels were first discovered in rod photoreceptors, where they are responsible for the primary electrical signal of the…”
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Using protein backbone mutagenesis to dissect the link between ion occupancy and C-type inactivation in K⁺ channels by Matulef, Kimberly, Komarov, Alexander G., Costantino, Corey A., Valiyaveetil, Francis I.

“…K ⁺ channels distinguish K ⁺ from Na ⁺ in the selectivity filter, which consists of four ion-binding sites (S1–S4, extracellular to intracellular) that are…”
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A facile approach for incorporating tyrosine esters to probe ion-binding sites and backbone hydrogen bonds by Reddi, Ravikumar, Chatterjee, Satyaki, Matulef, Kimberly, Gustafson, Andrew, Gao, Lujia, Valiyaveetil, Francis I

“…Amide-to-ester substitutions are used to study the role of the amide bonds of the protein backbone in protein structure, function, and folding. An amber…”
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Structures of Gating Intermediates in a K+ channel by Reddi, Ravikumar, Matulef, Kimberly, Riederer, Erika, Moenne-Loccoz, Pierre, Valiyaveetil, Francis I.

“…[Display omitted] •Ion flux through a K+ channel is regulated by channel activation and inactivation.•W67F in KcsA prevents inactivation and alters pH…”
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Structural Snapshots of Intermediates in the Gating of a K+ Channel by Reddi, Ravikumar, Matulef, Kimberly, Riederer, Erika, Moenne-Loccoz, Pierre, Valiyaveetil, Francis I.

“…Regulation of ion conduction through the pore of a K + channel takes place through the coordinated action of the activation gate at the bundle crossing of the…”
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Patch-Clamp Recordings of the KcsA K + Channel in Unilamellar Blisters by Matulef, Kimberly, Valiyaveetil, Francis I

“…Patch-clamp electrophysiology is the standard technique used for the high-resolution functional measurements on ion channels. While studies using patch clamp…”
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Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression by Focke, Paul J, Hein, Christopher, Hoffmann, Beate, Matulef, Kimberly, Bernhard, Frank, Dötsch, Volker, Valiyaveetil, Francis I

“…Cell free protein synthesis (CFPS) has emerged as a promising methodology for protein expression. While polypeptide production is very reliable and efficient…”
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Side-Dependent Inhibition of a Prokaryotic ClC by DIDS by Matulef, Kimberly, Maduke, Merritt

“…The x-ray structure of the Escherichia coli chloride/proton antiporter ClC-ec1 provides a structural paradigm for the widespread and diverse ClC family of…”
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TRPV1, TRPA1, and TRPM8 are expressed in axon terminals in the cornea: TRPV1 axons contain CGRP and secretogranin II; TRPA1 axons contain secretogranin 3 by Schecterson, Leslayann C, Pazevic, Alexander A, Yang, Ruian, Matulef, Kimberly, Gordon, Sharona E

“…The cornea is highly enriched in sensory neurons expressing the thermal TRP channels TRPV1, TRPA1, and TRPM8, and is an accessible tissue for study and…”
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The Role of Backbone Hydrogen Bonds in the Voltage Sensor of K+ Channels by Infield, Daniel T., Matulef, Kimberly, Galpin, Jason D., Ahern, Christopher A., Valiyaveetil, Francis I.

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Individual Ion Binding Sites in the K+ Channel Play Distinct Roles in C-type Inactivation and in Recovery from Inactivation by Matulef, Kimberly, Annen, Alvin W., Nix, Jay C., Valiyaveetil, Francis I.

“…The selectivity filter of K+ channels contains four ion binding sites (S1–S4) and serves dual functions of discriminating K+ from Na+ and acting as a gate…”
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Structural basis for C-type inactivation in a Shaker family voltage-gated K + channel by Reddi, Ravikumar, Matulef, Kimberly, Riederer, Erika A, Whorton, Matthew R, Valiyaveetil, Francis I

“…C-type inactivation is a process by which ion flux through a voltage-gated K (K ) channel is regulated at the selectivity filter. While prior studies have…”
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The CLC 'chloride channel' family: revelations from prokaryotes by Matulef, Kimberly, Maduke, Merritt

“…Members of the CLC 'chloride channel' family play vital roles in a wide variety of physiological settings. Research on prokaryotic CLC homologues provided…”
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Protein Backbone Mutagenesis Reveals a Novel Link between Ion Occupancy and C-type Inactivation in K+ Channels by Matulef, Kimberly, Komarov, Alexander G., Valiyaveetil, Francis I.

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