Search Results - "Matsuhisa, Akio"

Antioxidant vitamins and lysophospholipids are critical for inducing mouse spermatogenesis under organ culture conditions by Sanjo, Hiroyuki, Yao, Tatsuma, Katagiri, Kumiko, Sato, Takuya, Matsumura, Takafumi, Komeya, Mitsuru, Yamanaka, Hiroyuki, Yao, Masahiro, Matsuhisa, Akio, Asayama, Yuta, Ikeda, Kazutaka, Kano, Kuniyuki, Aoki, Junken, Arita, Makoto, Ogawa, Takehiko

“…In vitro mouse spermatogenesis using a classical organ culture method became possible by supplementing basal culture medium with only the product of bovine…”
Get full text

In vitro mouse spermatogenesis with an organ culture method in chemically defined medium by Sanjo, Hiroyuki, Komeya, Mitsuru, Sato, Takuya, Abe, Takeru, Katagiri, Kumiko, Yamanaka, Hiroyuki, Ino, Yoko, Arakawa, Noriaki, Hirano, Hisashi, Yao, Tatsuma, Asayama, Yuta, Matsuhisa, Akio, Yao, Masahiro, Ogawa, Takehiko

“…We previously reported the successful induction and completion of mouse spermatogenesis by culturing neonatal testis tissues. The culture medium consisted of…”
Get full text

Single human sperm cryopreservation method using hollow-core agarose capsules by Araki, Yasuyuki, Ph.D, Yao, Tatsuma, M.S, Asayama, Yuta, M.S, Matsuhisa, Akio, Ph.D, Araki, Yasuhisa, Ph.D

“…Objective To develop an efficient cryopreservation method using a single sperm. Design Experimental study. Setting Laboratory of a private institute…”
Get full text

Human Intelectin Is a Novel Soluble Lectin That Recognizes Galactofuranose in Carbohydrate Chains of Bacterial Cell Wall by Tsuji, Shoutaro, Uehori, Junji, Matsumoto, Misako, Suzuki, Yasuhiko, Matsuhisa, Akio, Toyoshima, Kumao, Seya, Tsukasa

“…Galactofuranosyl residues are present in various microorganisms but not in mammals. In this study, we identified a human lectin binding to galactofuranosyl…”
Get full text

Development of a new in situ hybridization method for the detection of global bacterial DNA to provide early evidence of a bacterial infection in spontaneous bacterial peritonitis by Enomoto, Hirayuki, Inoue, Shin-ichi, Matsuhisa, Akio, Aizawa, Nobuhiro, Imanishi, Hiroyasu, Saito, Masaki, Iwata, Yoshinori, Tanaka, Hironori, Ikeda, Naoto, Sakai, Yoshiyuki, Takashima, Tomoyuki, Shimomura, Soji, Iijima, Hiroko, Nakamura, Hideji, Nishiguchi, Shuhei

“…Background & Aims Despite the importance of identifying the causative pathogen(s), ascitic fluid cultures are occasionally negative in patients with…”
Get full text

Combinational approach using in situ hybridization targeting 23S ribosomal RNA genes and blood cultures for bacterial identification in patients with neutropenia and fever by Koh, Hideo, M.D., Ph.D, Aimoto, Mizuki, M.D., Ph.D, Matsuhisa, Akio, Ph.D, Inoue, Shin-Ichi, M.Sc, Katayama, Takako, Okamura, Hiroshi, M.D, Yoshimura, Takuro, M.D., Ph.D, Koh, Shiro, M.D., Ph.D, Nanno, Satoru, M.D, Nishimoto, Mitsutaka, M.D., Ph.D, Nakashima, Yasuhiro, M.D., Ph.D, Hirose, Asao, M.D., Ph.D, Nakamae, Mika, M.D., Ph.D, Nakane, Takahiko, M.D., Ph.D, Hino, Masayuki, M.D., Ph.D, Nakamae, Hirohisa, M.D., Ph.D

“…Abstract Background A new 23S ribosomal RNA genes-targeted in situ hybridization (ISH) probe to detect global bacterial genomic DNA (59 species from 35 genera;…”
Get full text

A Cytolethal Distending Toxin Gene-Based Multiplex PCR Assay for Detection of Campylobacter spp. in Stool Specimens and Comparison with Culture Method by SHIRAMARU, Sachi, ASAKURA, Masahiro, INOUE, Haruna, NAGITA, Akira, MATSUHISA, Akio, YAMASAKI, Shinji

“…In this study, we evaluated the applicability of cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR for the direct detection and…”
Get full text

Comparative analysis of cytolethal distending toxin ( cdt) genes among Campylobacter jejuni, C. coli and C. fetus strains by Asakura, Masahiro, Samosornsuk, Worada, Taguchi, Masumi, Kobayashi, Kazuhiro, Misawa, Naoaki, Kusumoto, Masahiro, Nishimura, Kazuhiko, Matsuhisa, Akio, Yamasaki, Shinji

“…The cytolethal distending toxin ( cdt) gene clusters of Campylobacter coli strain Co1-243 and C. fetus strain Col-187 were cloned and sequenced to understand…”
Get full text

Diagnosis of Spontaneous Bacterial Peritonitis and an ＂In Situ＂ Hybridization Approach to Detect an ＂Unidentified＂ Pathogen by Enomoto, Hirayuki, Inoue, Shin-ichi, Matsuhisa, Akio, Nishiguchi, Shuhei

“…Spontaneous bacterial peritonitis (SBP) is a frequent and severe complication in cirrhotic patients with ascites. Although identifying the pathogen(s) plays a…”
Get full text

Evaluation of a PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Assay for Molecular Epidemiological Study of Shiga Toxin-Producing Escherichia coli by SUGIMOTO, Norihiko, SHIMA, Kensuke, HINENOYA, Atsushi, ASAKURA, Masahiro, MATSUHISA, Akio, WATANABE, Haruo, YAMASAKI, Shinji

“…In this study, we have evaluated our recently developed polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay for the molecular…”
Get full text

Sepsis--diagnostic approaches for bacterial sepsis by Matsuhisa, Akio

Get more information

Tol1, a Fission Yeast Phosphomonoesterase, Is an In Vivo Target of Lithium, and Its Deletion Leads to Sulfite Auxotrophy by Miyamoto, R, Sugiura, R, Kamitani, S, Yada, T, Lu, Y, Sio, S O, Asakura, M, Matsuhisa, A, Shuntoh, H, Kuno, T

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
Get full text

Amplification of bacterial genomic DNA from all ascitic fluids with a highly sensitive polymerase chain reaction by Enomoto, Hirayuki, Inoue, Shin-Ichi, Matsuhisa, Akio, Iwata, Yoshinori, Aizawa, Nobuhiro, Sakai, Yoshiyuki, Takata, Ryo, Ikeda, Naoto, Hasegawa, Kunihiro, Nakano, Chikage, Nishimura, Takashi, Yoh, Kazunori, Miyamoto, Yuho, Ishii, Noriko, Yuri, Yukihisa, Ishii, Akio, Takashima, Tomoyuki, Nishikawa, Hiroki, Iijima, Hiroko, Nishiguchi, Shuhei

“…Due to varying positive rates of polymerase chain reaction (PCR) amplification, interpretation of conventional PCR results for non‑infectious ascites remains…”
Get full text

A simple staining method for dna and rna blotted on a membrane using a polyethyleneimine-enzyme conjugate by Matsuhisa, A, Kishi, Y, Shiba, K

“…We describe a new method for the detection of nucleic acids fixed on a membrane involving enzyme-conjugated polyethyleneimine. This method is based on the…”
Get more information

Detection of Bacteria in Phagocyte-Smears from Septicemia-Suspected Blood by In Situ Hybridization Using Biotinylated Probes by Matsuhisa, Akio, Saito, Yoshihiro, Sakamoto, Yoshimasa, Keshi, Hiroyuki, Ueyama, Hiroshi, Aikawa, Youko, Kishi, Youichiro, Ohno, Tsuneya

“…We report herein the detection of intracellular bacteria in phagocyte-smears obtained from septicemia-suspected blood samples by in situ hybridization. This…”
Get full text

Development of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection and identification of Campylobacter jejuni, Campylobacter coli and Campylobacter fetus by Asakura, Masahiro, Samosornsuk, Worada, Hinenoya, Atsushi, Misawa, Naoaki, Nishimura, Kazuhiko, Matsuhisa, Akio, Yamasaki, Shinji

“…A cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection of cdtA, cdtB or cdtC gene of Campylobacter jejuni,…”
Get full text

Binding of Streptavidin to Bacteria or Fungi and Its Applications in Detecting These Microbes by Matsuhisa, Akio, Saito, Yoshihiro, Ueyama, Hiroshi, Yamamoto, Makiko, Ohono, Tsuneya

“…We have investigated the characteristics and utilities of streptavidin-binding to gram-negative and gram-positive bacteria and Candida spp. The pre-treatment…”
Get full text

Detection of Staphylococci in mouse phagocytic cells by in situ hybridization using biotinylated DNA probes by Matsuhisa, A, Saito, Y, Ueyama, H, Aikawa, Y, Ohono, T

“…In situ hybridization was used to detect intracellular Staphylococcus aureus and S. epidermidis in mouse phagocytic cells after experimental infection of C3H…”
Get more information

Assignment of diagnostic approaches for bacterial sepsis by Matsuhisa, Akio, Hoshina, Sadayori

Get more information

DETECTION OF HUMAN PAPILLOMAVIRUS 16 AND 18 DNA IN CERVICAL DYSPLASIA AND CERVICAL CARCINOMA BY IN SITU HYBRIDIZATION by FUJITA, KAZUNOBU, TANAKA, HISAKO, MATSUHISA, AKIO, MATSUI, TAKAAKI, NIIMURA, MICHIHITO, OHNO, TSUNEYA

“…We improved the in situ hybridization method using biotinylatyed probes for the detection of human papillomavirus (HPV) 16 and 18 DNA in formalin-fixed and…”
Get full text