MuSK autoantibodies in myasthenia gravis detected by cell based assay — A multinational study

Abstract Seronegative myasthenia gravis (MG) presents a serious gap in MG diagnosis and understanding. We applied a cell based assay (CBA) for the detection of muscle specific kinase (MuSK) antibodies undetectable by radioimmunoassay. We tested 633 triple-seronegative MG patients' sera from 13...

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Bibliographic Details
Published in:	Journal of neuroimmunology Vol. 284; pp. 10 - 17
Main Authors:	Tsonis, A.I, Zisimopoulou, P, Lazaridis, K, Tzartos, J, Matsigkou, E, Zouvelou, V, Mantegazza, R, Antozzi, C, Andreetta, F, Evoli, A, Deymeer, F, Saruhan-Direskeneli, G, Durmus, H, Brenner, T, Vaknin, A, Berrih-Aknin, S, Behin, A, Sharshar, T, De Baets, M, Losen, M, Martinez-Martinez, P, Kleopa, K.A, Zamba-Papanicolaou, E, Kyriakides, T, Kostera-Pruszczyk, A, Szczudlik, P, Szyluk, B, Lavrnic, D, Basta, I, Peric, S, Tallaksen, C, Maniaol, A, Casasnovas Pons, C, Pitha, J, Jakubíkova, M, Hanisch, F, Tzartos, S.J
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 15-07-2015
Subjects:	Adult Aged Allergy and Immunology Autoantibodies Autoantibodies - blood Cell based assay Diagnosis Female Flow Cytometry Humans International Cooperation LDL-Receptor Related Proteins - immunology Male Middle Aged MuSK Myasthenia gravis Myasthenia Gravis - blood Myasthenia Gravis - diagnosis Myasthenia Gravis - pathology Neurology Neuromyelitis Optica - diagnosis Radioimmunoassay Receptor Protein-Tyrosine Kinases - immunology Receptors, Cholinergic - immunology Thymus Gland - pathology Thymus Hyperplasia - diagnosis acetylcholine receptor AChR low-density lipoprotein receptor-related protein 4 CBA Autoantibodies MuSK myasthenia gravis LRP4 MG muscle specific kinase Diagnosis cell based assay Myasthenia gravis Cell based assay
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Summary:	Abstract Seronegative myasthenia gravis (MG) presents a serious gap in MG diagnosis and understanding. We applied a cell based assay (CBA) for the detection of muscle specific kinase (MuSK) antibodies undetectable by radioimmunoassay. We tested 633 triple-seronegative MG patients' sera from 13 countries, detecting 13% as positive. MuSK antibodies were found, at significantly lower frequencies, in 1.9% of healthy controls and 5.1% of other neuroimmune disease patients, including multiple sclerosis and neuromyelitis optica. The clinical data of the newly diagnosed MuSK-MG patients are presented. 27% of ocular seronegative patients were MuSK antibody positive. Moreover, 23% had thymic hyperplasia suggesting that thymic abnormalities are more common than believed.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0165-5728 1872-8421
DOI:	10.1016/j.jneuroim.2015.04.015