Calcium-binding protein, spermatid-specific 1 is expressed in human salivary glands and contains an anti-inflammatory motif

Salivary glands are involved in the production and exocrine and endocrine secretion of biologically active proteins, polypeptides, and hormones involved in growth and differentiation, homeostasis, and digestion. We have previously studied the prohormone submandibular rat 1 (SMR1), product of the Vcs...

Full description

Saved in:

Bibliographic Details
Published in:	American journal of physiology. Regulatory, integrative and comparative physiology Vol. 308; no. 7; p. R569
Main Authors:	St Laurent, Chris D, St Laurent, Katherine E, Mathison, Ron D, Befus, A Dean
Format:	Journal Article
Language:	English
Published:	United States 01-04-2015
Subjects:	Amino Acid Motifs Anaphylaxis - chemically induced Anaphylaxis - immunology Anaphylaxis - metabolism Anaphylaxis - prevention & control Animals Anti-Inflammatory Agents - pharmacology Calcium-Binding Proteins - genetics Calcium-Binding Proteins - metabolism Calcium-Binding Proteins - pharmacology Disease Models, Animal Dose-Response Relationship, Drug Gene Expression Regulation Humans Lipopolysaccharides Male Mice, Inbred BALB C Neutrophil Infiltration - drug effects Neutrophils - drug effects Neutrophils - immunology Neutrophils - metabolism Ovalbumin Peptide Fragments - metabolism Peptide Fragments - pharmacology Pneumonia - chemically induced Pneumonia - immunology Pneumonia - metabolism Pneumonia - prevention & control Protein Precursors - metabolism Rats, Sprague-Dawley RNA, Messenger - metabolism Salivary Glands - metabolism Salivary Proteins and Peptides - genetics Salivary Proteins and Peptides - metabolism Salivary Proteins and Peptides - pharmacology saliva neutrophilia Vcsa1 inflammation SMR1
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Salivary glands are involved in the production and exocrine and endocrine secretion of biologically active proteins, polypeptides, and hormones involved in growth and differentiation, homeostasis, and digestion. We have previously studied the prohormone submandibular rat 1 (SMR1), product of the Vcsa1 gene, which is highly expressed in the testes and salivary glands of rats, and can be cleaved to produce polypeptides with analgesic, erectile function, and anti-inflammatory activities. Humans lack the Vcsa1 gene, but homologous sequences and functions for analgesia and erectile function exist in the human genes Prol1, SMR3a, and SMR3b located on the human chromosomal region close to where Vcsa1 lies in the rat. Here we show the human protein calcium-binding protein spermatid-specific 1 (CABS1) contains a similar sequence to the anti-inflammatory sequence in rat SMR1, thus CABS1 may be another human gene with homologous function to Vcsa1. Using Western blot and PCR, we discovered that the human protein CABS1, previously thought to only be expressed in the testes, is also expressed in the salivary glands and lung, in a tissue-specific manner. Peptides derived from CABS1 were tested in an in vivo mouse model of lipopolysaccharide (LPS)-induced neutrophilia and an ex vivo rat model of antigen-induced intestinal anaphylaxis and significantly reduced both neutrophil accumulation in bronchoalveolar lavage fluid and antigen-induced ileal contractions, respectively. Thus human CABS1 has a peptide motif homologous to the anti-inflammatory peptide sequence of rat SMR1. Whether this similarity of CABS1 extends to the neuroendocrine regulation of the anti-inflammatory activity seen for SMR1 remains to be determined.
ISSN:	1522-1490
DOI:	10.1152/ajpregu.00153.2014