Cell-autonomous requirement for DNaseII in nonapoptotic cell death

Cell-autonomous requirement for DNaseII in nonapoptotic cell death

DNA fragmentation is a critical component of apoptosis but it has not been characterized in nonapoptotic forms of cell death, such as necrosis and autophagic cell death. In mammalian apoptosis, caspase-activated DNase cleaves DNA into nucleosomal fragments in dying cells, and subsequently DNase II,...

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Bibliographic Details
Published in:Cell death and differentiation Vol. 16; no. 10; pp. 1362 - 1371
Main Authors: Bass, B P, Tanner, E A, Mateos San Martín, D, Blute, T, Kinser, R D, Dolph, P J, McCall, K
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 01-10-2009
Nature Publishing Group
Subjects:
Animals
Apoptosis
Autophagy
Biochemistry
Biomedical and Life Sciences
Cell Biology
Cell Cycle Analysis
Cell death
Cell Death - physiology
Drosophila melanogaster - cytology
Drosophila melanogaster - enzymology
Endodeoxyribonucleases - metabolism
Fasting
Life Sciences
Lysosomes - metabolism
Oocytes - cytology
Oogenesis
original-paper
Stem Cells
DNase II
ovary
autophagic cell death
necrosis
lysosome
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Summary:DNA fragmentation is a critical component of apoptosis but it has not been characterized in nonapoptotic forms of cell death, such as necrosis and autophagic cell death. In mammalian apoptosis, caspase-activated DNase cleaves DNA into nucleosomal fragments in dying cells, and subsequently DNase II, an acid nuclease, completes the DNA degradation but acts non-cell autonomously within lysosomes of engulfing cells. Here we examine the requirement for DNases during two examples of programmed cell death (PCD) that occurs in the Drosophila melanogaster ovary, starvation-induced death of mid-stage egg chambers and developmental nurse cell death in late oogenesis. Surprisingly, we found that DNaseII was required cell autonomously in nurse cells during developmental PCD, indicating that it acts within dying cells. Dying nurse cells contain autophagosomes, indicating that autophagy may contribute to these forms of PCD. Furthermore, we provide evidence that developmental nurse cell PCD in late oogenesis shows hallmarks of necrosis. These findings indicate that DNaseII can act cell autonomously to degrade DNA during nonapoptotic cell death.
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ISSN:1350-9047
1476-5403
DOI:10.1038/cdd.2009.79