Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks

Several species are among the most important cultivated stone fruits in the Mediterranean region, and there is an urgent need to obtain rootstocks with specific adaptations to challenging environmental conditions. The development of adventitious roots (ARs) is an evolutionary mechanism of high relev...

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Bibliographic Details
Published in:	Plants (Basel) Vol. 11; no. 7; p. 913
Main Authors:	Justamante, María Salud, Mhimdi, Mariem, Molina-Pérez, Marta, Albacete, Alfonso, Moreno, María Ángeles, Mataix, Inés, Pérez-Pérez, José Manuel
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 29-03-2022 MDPI
Subjects:	Acetic acid Adaptation Aspartic acid auxin homeostasis Auxins Butyric acid Callus Cultivation Environmental conditions Flowers & plants Fruit cultivation Fruits Genomes Genotypes Hardwoods Homeostasis hormone profiling Hydroponics Indole-3-butyric acid Indoleacetic acid Metabolites Molecular modelling Nutrients Prunus Prunus rootstocks Rooting Rootstocks Stone Trees vegetative propagation Spain auxin homeostasis Prunus rootstocks vegetative propagation hormone profiling
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Summary:	Several species are among the most important cultivated stone fruits in the Mediterranean region, and there is an urgent need to obtain rootstocks with specific adaptations to challenging environmental conditions. The development of adventitious roots (ARs) is an evolutionary mechanism of high relevance for stress tolerance, which has led to the development of environmentally resilient plants. As a first step towards understanding the genetic determinants involved in AR formation in sp., we evaluated the rooting of hardwood cuttings from five rootstocks (Adafuel, Adarcias, Cadaman, Garnem, and GF 677) grown in hydroponics. We found that auxin-induced callus and rooting responses were strongly genotype-dependent. To investigate the molecular mechanisms involved in these differential responses, we performed a time-series study of AR formation in two rootstocks with contrasting rooting performance, Garnem and GF 677, by culturing in vitro microcuttings with and without auxin treatment (0.9 mg/L of indole-3-butyric acid [IBA]). Despite showing a similar histological structure, Garnem and GF677 rootstocks displayed dynamic changes in endogenous hormone homeostasis involving metabolites such as indole-3-acetic acid (IAA) conjugated to aspartic acid (IAA-Asp), and these changes could explain the differences observed during rooting.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. Present address: Departamento de Producción Vegetal y Agrotecnología, Instituto Murciano de Investigación y Desarrollo Agrario y Medioambiental (IMIDA), 30150 Alberca Las Torres, Spain.
ISSN:	2223-7747 2223-7747
DOI:	10.3390/plants11070913