HPLC Determination of Ochratoxin A in a Low Volume of Human Blood Serum

A high-performance liquid chromatography (HPLC) method has been developed for the determination of ochratoxin A (OTA) in human blood serum. Samples were purified on a C18 solid phase extraction column. The developed method required a relatively low serum volume (0.5 ml). Significant correlation (r o...

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Bibliographic Details
Published in:	Analytical letters Vol. 41; no. 5; pp. 757 - 766
Main Authors:	Ghali, R., Hmaissia-Khlifa, K., Mezigh, Ch, Ghorbel, H., Maaroufi, K., Maschgoul, S., Hedilli, A.
Format:	Journal Article
Language:	English
Published:	Philadelphia, PA Taylor & Francis Group 01-04-2008 Taylor & Francis
Subjects:	Analytical chemistry Chemistry Chromatographic methods and physical methods associated with chromatography Exact sciences and technology HPLC Ochratoxin A Other chromatographic methods serum SPE Tunisia Performance evaluation Human Solid phase extraction HPLC chromatography SPE Ochratoxin A Blood Chemical enrichment Accuracy Detection limit Serum Tunisia HPLC
Online Access:	Get full text
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Summary:	A high-performance liquid chromatography (HPLC) method has been developed for the determination of ochratoxin A (OTA) in human blood serum. Samples were purified on a C18 solid phase extraction column. The developed method required a relatively low serum volume (0.5 ml). Significant correlation (r of 0.998) was found over the range from 0.10 to 8 ng/ml, with a detection limit of 0.1 ng/ml and better performance in terms of precision and accuracy. Mean recoveries at 0.5 and 2 ng/ml were respectively 69.7±1.2 and 71.9±2.8%. This method was used as a rapid and noninvasive tool to assess human exposure to OTA. Among 40 analyzed serum samples, 27.5% were found to contain OTA with levels going from 0.1 to 11.98 ng/ml with a mean concentration of 0.73±2.35 ng/ml.
ISSN:	0003-2719 1532-236X
DOI:	10.1080/00032710801934528