Search Results - "Martsev, Sergey P"

Folding of an antibody variable domain in two functional conformations in vitro: calorimetric and spectroscopic study of the anti-ferritin antibody VL domain by Tsybovsky, Yaroslav, Shubenok, Denis V., Kravchuk, Zinaida I., Martsev, Sergey P.

“…Understanding refolding pathways of recombinant antibody fragments is essential for efficient production of these proteins of high biomedical significance. The…”
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Amyloid Fibril Formation of the Mouse VL Domain at Acidic pH by Martsev, Sergey P, Dubnovitsky, Anatoly P, Vlasov, Alexander P, Hoshino, Masaru, Hasegawa, Kazuhiro, Naiki, Hironobu, Goto, Yuji

“…The recombinant VL domain that represents the variable part of the light chain (type κ) of mouse monoclonal antibody F11 directed against human spleen ferritin…”
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Fusion of the antiferritin antibody VL domain to barnase results in enhanced solubility and altered pH stability by Martsev, Sergey P., Tsybovsky, Yaroslav I., Stremovskiy, Oleg A., Odintsov, Sergey G., Balandin, Taras G., Arosio, Paolo, Kravchuk, Zinaida I., Deyev, Sergey M.

“…Chimeric immunotoxins that combine antigen recognition domains of antibodies and cytotoxic RNases have attracted much attention in recent years as potential…”
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Independent folding and conformational changes of the barnase module in the VL-barnase immunofusion: calorimetric evidence by Tsybovsky, Yaroslav I, Kedrov, Alexey A, Martsev, Sergey P

“…Although stability is critical for in vivo application of immunotoxins, a thermodynamic description of their folding/stability is still lacking. We applied…”
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Significance of serum immunoglobulin G for leukocytosis and prognosis in childhood B-lineage acute lymphoblastic leukemia by Potapnev, Michael P., Belevtsev, Michael V., Bortkevich, Ludmila G., Grinev, Vasily V., Martsev, Sergey P., Kravchuk, Zinaida I., Migal, Natalia V., Aleinikova, Olga V.

“…Background This study was conducted to evaluate the significance of serum level of immunoglobulins (Igs) and particularly IgG for leukemic cell persistence in…”
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Partially structured state of the functional VH domain of the mouse anti-ferritin antibody F11 by Martsev, Sergey P, Dubnovitsky, Anatoly P, Stremovsky, Oleg A, Chumanevich, Alexander A, Tsybovsky, Yaroslav I, Kravchuk, Zinaida I, Deyev, Sergey M

“…An antibody combining site generally involves the two variable domains, VH from the heavy and VL from the light chain. We expressed the individual VH domain of…”
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Thermodynamic and functional characterization of a stable IgG conformer obtained by renaturation from a partially structured low pH-induced state by Martsev, Sergey P., Kravchuk, Zinaida I., Vlasov, Alexander P., Lyakhnovich, Georgy V.

“…At pH 2, rabbit IgG adopts a partially structured state that exhibits loss of thermal unfolding transition, tentatively assigned to the CH2 domain, whilst…”
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Antiferritin Single-Chain Fv Fragment Is a Functional Protein with Properties of a Partially Structured State:  Comparison with the Completely Folded VL Domain by Martsev, Sergey P, Chumanevich, Alexander A, Vlasov, Alexander P, Dubnovitsky, Anatoly P, Tsybovsky, Yaroslav I, Deyev, Sergey M, Cozzi, Anna, Arosio, Paolo, Kravchuk, Zinaida I

“…Differential scanning calorimetry and spectroscopic probes were applied to study folding and stability of the single-chain Fv fragment (scFv) of the anti-human…”
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Large increase in thermal stability of the CH2 domain of rabbit IgG after acid treatment as evidenced by differential scanning calorimetry by Martsev, S P, Kravchuk, Z I, Vlasov, A P

“…Rabbit IgG after exposure to 0.05 M glycine-HCl, pH 2.0, and native IgG were compared by differential scanning calorimetry (DSC) at pH 3.5 and C1q binding…”
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Amyloid Fibril Formation of the Mouse V L Domain at Acidic pH by Martsev, Sergey P., Dubnovitsky, Anatoly P., Vlasov, Alexander P., Hoshino, Masaru, Hasegawa, Kazuhiro, Naiki, Hironobu, Goto, Yuji

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Antiferritin single-chain Fv fragment is a functional protein with properties of a partially structured state: comparison with the completely folded V(L) domain by Martsev, S P, Chumanevich, A A, Vlasov, A P, Dubnovitsky, A P, Tsybovsky, Y I, Deyev, S M, Cozzi, A, Arosio, P, Kravchuk, Z I

“…Differential scanning calorimetry and spectroscopic probes were applied to study folding and stability of the single-chain Fv fragment (scFv) of the anti-human…”
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Amyloid fibril formation of the mouse V(L) domain at acidic pH by Martsev, Sergey P, Dubnovitsky, Anatoly P, Vlasov, Alexander P, Hoshino, Masaru, Hasegawa, Kazuhiro, Naiki, Hironobu, Goto, Yuji

“…The recombinant V(L) domain that represents the variable part of the light chain (type kappa) of mouse monoclonal antibody F11 directed against human spleen…”
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Antiferritin Single-Chain Fv Fragment Is a Functional Protein with Properties of a Partially Structured State:  Comparison with the Completely Folded V L Domain by Martsev, Sergey P., Chumanevich, Alexander A., Vlasov, Alexander P., Dubnovitsky, Anatoly P., Tsybovsky, Yaroslav I., Deyev, Sergey M., Cozzi, Anna, Arosio, Paolo, Kravchuk, Zinaida I.

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Distinct stability of recombinant L and H subunits of human ferritin: calorimetric and ANS binding studies by Martsev, S P, Vlasov, A P, Arosio, P

“…Thermodynamic and pH stability of recombinant human L- and H-ferritins were probed by differential scanning calorimetry and 8-anilino-1-naphthalenesulfonate…”
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Antiferritin single-chain antibody: a functional protein with incomplete folding? by Martsev, Sergey P., Kravchuk, Zinaida I., Chumanevich, Alexander A., Vlasov, Alexander P., Dubnovitsky, Anatoly P., Bespalov, Ivan A., Arosio, Paolo, Deyev, Sergey M.

“…The pET(scF11) plasmid was constructed comprising the gene of a single-chain antibody against human ferritin. This plasmid encodes the leader peptide pelB…”
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Two high-affinity monoclonal IgG2a antibodies with differing thermodynamic stability demonstrate distinct antigen-induced changes in protein A-binding affinity by Kravchuk, Zinaida I., Chumanevich, Alexander A., Vlasov, Alexander P., Martsev, Sergey P.

“…Two IgG2a monoclonal antibodies (G10 and F11) are described which have similar affinity for human spleen ferritin and identical protein A-binding affinity. The…”
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Modification of monoclonal and polyclonal IgG with palladium (II) coproporphyrin I: stimulatory and inhibitory functional effects induced by two different methods by Martsev, Sergey P., Preygerzon, Valery A., Mel'nikova, Yanina I., Kravchuk, Zinaida I., Ponomarev, Gely V., Lunev, Vitaly E., Savitsky, Alexander P.

“…Antibodies conjugated with porphyrins and metalloporphyrins have a great potential for applications in fluorescence or phosphorescence immunoassays as well as…”
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