Nucleic acid cleavage by a Cu(II) polyaza macrocyclic complex

In this paper, we describe the activity of the [Cu 2BMXD] complex as a nucleic acid cleavage agent. The rate constant for the DNA backbone cleavage reaction was estimated to be 4.67 × 10 −2 h −1 at 50 °C, pH 7.0. We demonstrate that the DNA cleavage reaction promoted by this complex may occur via an...

Full description

Saved in:

Bibliographic Details
Published in:	Polyhedron Vol. 24; no. 4; pp. 495 - 499
Main Authors:	Oliveira, M.C.B., Couto, M.S.R., Severino, P.C., Foppa, T., Martins, G.T.S., Szpoganicz, B., Peralta, R.A., Neves, A., Terenzi, H.
Format:	Journal Article
Language:	English
Published:	Elsevier Ltd 10-03-2005
Subjects:	Binuclear copper complex BMXD Chemical nuclease DNA cleavage DNA–drug interaction Polyaza ligand BMXD Chemical nuclease DNA cleavage DNA–drug interaction Polyaza ligand Binuclear copper complex
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	In this paper, we describe the activity of the [Cu 2BMXD] complex as a nucleic acid cleavage agent. The rate constant for the DNA backbone cleavage reaction was estimated to be 4.67 × 10 −2 h −1 at 50 °C, pH 7.0. We demonstrate that the DNA cleavage reaction promoted by this complex may occur via an oxidative pathway. This study establishes [Cu 2BMXD] as a novel artificial nuclease. The search for efficient artificial nucleases is a consequence of the huge efforts towards DNA sequencing by genome projects around the world. In the present study, we describe the activity of the Cu(II)–BMXD complex (BMXD = 3,6,9,17,20,23-hexaazatricyclo[23.3.1.1 11,15]triaconta-1(29),11(30),12,14,25,27-hexaene) as a DNA cleavage agent. The rate constant for the DNA backbone cleavage reaction at room temperature (25 °C) and pH 7.0 was estimated to be 1.1 × 10 −6 s −1, which corresponds, approximately, to a half-life ( t 1/2) of one week. We demonstrated that the DNA cleavage reaction promoted by the Cu 2BMXD complex may occur via an oxidative pathway through performing reactions in the presence of free-radical acceptors and comparing the activity under aerobic and anaerobic conditions. The Cu 2BMXD complex reported here is established as a novel artificial nuclease.
ISSN:	0277-5387
DOI:	10.1016/j.poly.2004.12.016