Search Results - "Marshall Stark, W"

Site-specific recombinases: molecular machines for the Genetic Revolution by Olorunniji, Femi J, Rosser, Susan J, Stark, W Marshall

“…The fields of molecular genetics, biotechnology and synthetic biology are demanding ever more sophisticated molecular tools for programmed precise modification…”
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Structural basis for topological regulation of Tn3 resolvase by Montaño, Sherwin P, Rowland, Sally-J, Fuller, James R, Burke, Mary E, MacDonald, Alasdair I, Boocock, Martin R, Stark, W Marshall, Rice, Phoebe A

“…Site-specific DNA recombinases play a variety of biological roles, often related to the dissemination of antibiotic resistance, and are also useful synthetic…”
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Variable orthogonality of serine integrase interactions within the ϕC31 family by MacDonald, Alasdair I., Baksh, Aron, Holland, Alexandria, Shin, Heewhan, Rice, Phoebe A., Stark, W. Marshall, Olorunniji, Femi J.

“…Serine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage…”
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Genome Integration and Excision by a New Streptomyces Bacteriophage, ϕJoe by Fogg, Paul C M, Haley, Joshua A, Stark, W Marshall, Smith, Margaret C M

“…Bacteriophages are the source of many valuable tools for molecular biology and genetic manipulation. In , most DNA cloning vectors are based on serine…”
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Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination by Colloms, Sean D, Merrick, Christine A, Olorunniji, Femi J, Stark, W Marshall, Smith, Margaret C M, Osbourn, Anne, Keasling, Jay D, Rosser, Susan J

“…Synthetic biology requires effective methods to assemble DNA parts into devices and to modify these devices once made. Here we demonstrate a convenient rapid…”
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The protein–protein interactions required for assembly of the Tn3 resolution synapse by Rowland, Sally‐J., Boocock, Martin R., Burke, Mary E., Rice, Phoebe A., Stark, W. Marshall

“…The site‐specific recombinase Tn3 resolvase initiates DNA strand exchange when two res recombination sites and six resolvase dimers interact to form a synapse…”
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The Serine Recombinases by Stark, W Marshall

“…In site-specific recombination, two short DNA sequences ('sites') are each cut at specific points in both strands, and the cut ends are rejoined to new…”
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Mathematical model of a serine integrase-controlled toggle switch with a single input by Pokhilko, Alexandra, Ebenhöh, Oliver, Stark, W. Marshall, Colloms, Sean D.

“…Dual-state genetic switches that can change their state in response to input signals can be used in synthetic biology to encode memory and control gene…”
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Control of serine integrase recombination directionality by fusion with the directionality factor by Olorunniji, Femi J, McPherson, Arlene L, Rosser, Susan J, Smith, Margaret C M, Colloms, Sean D, Stark, W Marshall

“…Bacteriophage serine integrases are extensively used in biotechnology and synthetic biology for assembly and rearrangement of DNA sequences. Serine integrases…”
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Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending by Bebel, Aleksandra, Karaca, Ezgi, Kumar, Banushree, Stark, W Marshall, Barabas, Orsolya

“…Bacterial Xer site-specific recombinases play an essential genome maintenance role by unlinking chromosome multimers, but their mechanism of action has…”
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Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina by Gao, Hong, Murugesan, Buvani, Hoßbach, Janina, Evans, Stephanie K, Stark, W Marshall, Smith, Margaret C M

“…Few natural product pathways from rare Actinomycetes have been studied due to the difficulty in applying molecular approaches in these genetically intractable…”
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Purification and In Vitro Characterization of Zinc Finger Recombinases by Olorunniji, Femi J, Rosser, Susan J, Marshall Stark, W

“…Zinc finger recombinases (ZFRs) are designer site-specific recombinases that have been adapted for a variety of genome editing purposes. Due to their modular…”
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Structural Basis for Catalytic Activation of a Serine Recombinase by Keenholtz, Ross A., Rowland, Sally-J., Boocock, Martin R., Stark, W. Marshall, Rice, Phoebe A.

“…Sin resolvase is a site-specific serine recombinase that is normally controlled by a complex regulatory mechanism. A single mutation, Q115R, allows the enzyme…”
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Gated rotation mechanism of site-specific recombination by ΦC31 integrase by Olorunniji, Femi J., Buck, Dorothy E., Colloms, Sean D., McEwan, Andrew R., Smith, Margaret C. M., Stark, W. Marshall, Rosser, Susan J.

“…Integrases, such as that of the Streptomyces temperate bacteriophage ΦC31, promote site-specific recombination between DNA sequences in the bacteriophage and…”
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A simplified mathematical model of directional DNA site-specific recombination by serine integrases by Pokhilko, Alexandra, Zhao, Jia, Stark, W. Marshall, Colloms, Sean D., Ebenhöh, Oliver

“…Serine integrases catalyse site-specific recombination to integrate and excise bacteriophage genomes into and out of their host's genome. These enzymes exhibit…”
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Making serine integrases work for us by Stark, W Marshall

“…Highlights • Serine integrases are becoming popular tools for research and biotechnology. • Some applications (such as genetic logic systems) require several…”
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Zinc finger recombinases with adaptable DNA sequence specificity by Proudfoot, Chris, McPherson, Arlene L, Kolb, Andreas F, Stark, W Marshall

“…Site-specific recombinases have become essential tools in genetics and molecular biology for the precise excision or integration of DNA sequences. However,…”
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Chimeric Recombinases with Designed DNA Sequence Recognition by Akopian, Aram, He, Jiuya, Boocock, Martin R., Stark, W. Marshall

“…Site-specific recombination typically occurs only between DNA sequences that have co-evolved with a natural recombinase enzyme to optimize sequence…”
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Synapsis and catalysis by activated Tn3 resolvase mutants by Olorunniji, Femi J., He, Jiuya, Wenwieser, Sandra V.C.T., Boocock, Martin R., Stark, W. Marshall

“…The serine recombinase Tn3 resolvase catalyses recombination between two 114 bp res sites, each of which contains binding sites for three resolvase dimers. We…”
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Intermediates in serine recombinase-mediated site-specific recombination by Marshall Stark, W, Boocock, Martin R, Olorunniji, Femi J, Rowland, Sally-J

“…Site-specific recombinases are enzymes that promote precise rearrangements of DNA sequences. They do this by cutting and rejoining the DNA strands at specific…”
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