Cross-reactivity of Shigella flexneri serotype 2a O antigen antibodies following immunization or infection

To study the cross-reactivity pattern of Shigella flexneri 2a O-antigen antibodies, sera from humans and monkeys challenged with S. flexneri 2a, and from humans and guinea pigs immunized with a recombinant vaccine expressing serotype 2a O-antigen, were tested against a panel of lipopolysaccharide ex...

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Bibliographic Details
Published in:	Vaccine Vol. 14; no. 11; pp. 1062 - 1068
Main Authors:	Van De Verg, Lillian L., Bendiuk, Natalie O., Kotloff, Karen, Marsh, Michelle M., Ruckert, Jennifer L., Puryear, Jill L., Taylor, David N., Hartman, Antoinette B.
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 01-08-1996 Elsevier
Subjects:	Adult Animals antibodies Antibodies, Bacterial - blood Antibodies, Bacterial - immunology Bacterial Vaccines - immunology Bacteriology Biological and medical sciences Carbohydrate Sequence Cross Reactions cross-reactivity Dysentery, Bacillary - immunology Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Guinea Pigs Haplorhini Humans Immunoglobulin A - blood Immunoglobulin A - immunology Immunoglobulin G - blood Immunoglobulin G - immunology Lipopolysaccharides - immunology LPS Microbiology Molecular Sequence Data O Antigens - immunology Shigella flexneri Shigella flexneri - classification Shigella flexneri - immunology Shigella flexneri 2a vaccine Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, Synthetic - immunology vaccine antibodies cross-reactivity Shigella flexneri 2a LPS Human Immunization Antibody Shigella flexneri Vaccine Polyvalent vaccine Infection O antigen Immunogenicity Animal Cross reaction Bacteria Serotype Enterobacteriaceae
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Summary:	To study the cross-reactivity pattern of Shigella flexneri 2a O-antigen antibodies, sera from humans and monkeys challenged with S. flexneri 2a, and from humans and guinea pigs immunized with a recombinant vaccine expressing serotype 2a O-antigen, were tested against a panel of lipopolysaccharide extracted from heterologous S. flexneri. Sera from the two groups of humans, who were volunteers in either a clinical challenge or vaccination study, showed similar patterns: cross-reactivity was more often seen with IgA antibodies, and these were mostly cross-reactive with serotype 2b, which shares the type II antigen, and serotypes 1a, 5a, and Y, which share 4 or 3,4 group antigen, with 2a. The majority of sera from immunized guinea pigs showed both IgG and IgA cross-reactivity with 1a, 5a, and Y, but not 2b. The majority of sera from challenged monkeys showed cross-reactivity with almost all flexneri serotypes tested, with 1a, 2b, and Y being recognized most often, and the cross-reactive antibodies were more often IgG than IgA. These results show that either immunization or challenge with the 2a serotype induces cross-reactive antibodies which recognize similar subsets of heterologous serotypes, and suggest that it may be possible to design multivalent vaccines against S. flexneri.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0264-410X 1873-2518
DOI:	10.1016/0264-410X(96)00006-0