Search Results - "Marsh, Brad J"

Journeys through the Golgi--taking stock in a new era by Emr, Scott, Glick, Benjamin S, Linstedt, Adam D, Lippincott-Schwartz, Jennifer, Luini, Alberto, Malhotra, Vivek, Marsh, Brad J, Nakano, Akihiko, Pfeffer, Suzanne R, Rabouille, Catherine, Rothman, James E, Warren, Graham, Wieland, Felix T

“…The Golgi apparatus is essential for protein sorting and transport. Many researchers have long been fascinated with the form and function of this organelle…”
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Expedited approaches to whole cell electron tomography and organelle mark-up in situ in high-pressure frozen pancreatic islets by Noske, Andrew B., Costin, Adam J., Morgan, Garry P., Marsh, Brad J.

“…We have developed a simplified, efficient approach for the 3D reconstruction and analysis of mammalian cells in toto by electron microscope tomography (ET), to…”
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Islet Cholesterol Accumulation Due to Loss of ABCA1 Leads to Impaired Exocytosis of Insulin Granules by KRUIT, Janine K, WIJESEKARA, Nadeeja, JOHNSON, James D, LIGHT, Peter E, MARSH, Brad J, MACDONALD, Patrick E, BRUCE VERCHERE, C, HAYDEN, Michael R, MANNING FOX, Jocelyn E, DAI, Xiao-Qing, BRUNHAM, Liam R, SEARLE, Gavin J, MORGAN, Garry P, COSTIN, Adam J, RENMEI TANG, BHATTACHARJEE, Alpana

“…The ATP-binding cassette transporter A1 (ABCA1) is essential for normal insulin secretion from β-cells. The aim of this study was to elucidate the mechanisms…”
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High-Resolution 3D Quantitative Analysis of Caveolar Ultrastructure and Caveola-Cytoskeleton Interactions by Richter, Tobias, Floetenmeyer, Matthias, Ferguson, Charles, Galea, Janette, Goh, Jaclyn, Lindsay, Margaret R, Morgan, Garry P, Marsh, Brad J, Parton, Robert G

“…Caveolae are characteristic invaginations of the mammalian plasma membrane (PM) implicated in lipid regulation, signal transduction and endocytosis. We have…”
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Electron Tomography Reveals Rab6 Is Essential to the Trafficking of trans‐Golgi Clathrin and COPI‐Coated Vesicles and the Maintenance of Golgi Cisternal Number by Storrie, Brian, Micaroni, Massimo, Morgan, Garry P., Jones, Nick, Kamykowski, Jeffrey A., Wilkins, Ngozi, Pan, Timothy H., Marsh, Brad J.

“…We have shown previously that Rab6, a small, trans‐Golgi‐localized GTPase, acts upstream of the conserved oligomeric Golgi complex (COG) and ZW10/RINT1…”
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Rab6a/a' are important Golgi regulators of pro-inflammatory TNF secretion in macrophages by Micaroni, Massimo, Stanley, Amanda C, Khromykh, Tatiana, Venturato, Juliana, Wong, Colin X F, Lim, Jet P, Marsh, Brad J, Storrie, Brian, Gleeson, Paul A, Stow, Jennifer L

“…Lipopolysaccharide (LPS)-activated macrophages secrete pro-inflammatory cytokines, including tumor necrosis factor (TNF) to elicit innate immune responses…”
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A Cholesterol‐Dependent Endocytic Mechanism Generates Midbody Tubules During Cytokinesis by Kettle, Emma, Page, Scott L., Morgan, Garry P., Malladi, Chandra S., Wong, Chin L., Boadle, Ross A., Marsh, Brad J., Robinson, Phillip J., Chircop, Megan

“…Electron tomography reveals that many vesicles within the intracellular bridge (ICB) of a cytokinetic cell are not independent but connected, forming a newly…”
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Direct Continuities between Cisternae at Different Levels of the Golgi Complex in Glucose-Stimulated Mouse Islet Beta Cells by Marsh, Brad J., Volkmann, Niels, McIntosh, J. Richard, Howell, Kathryn E., Bjorkman, Pamela J.

“…Direct continuity between the membranes of cisternae in the Golgi complex in mammalian cells rarely has been observed; when seen, its documentation has been…”
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Lessons from tomographic studies of the mammalian Golgi by Marsh, Brad J.

“…Basic structure studies of the biosynthetic machinery of the cell by electron microscopy (EM) have underpinned much of our fundamental knowledge in the areas…”
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Organellar Relationships in the Golgi Region of the Pancreatic Beta Cell Line, HIT-T15, Visualized by High Resolution Electron Tomography by Marsh, Brad J., Mastronarde, David N., Buttle, Karolyn F., Howell, Kathryn E., McIntosh, J. Richard

“…The positional relationships among all of the visible organelles in a densely packed region of cytoplasm from an insulin secreting, cultured mammalian cell…”
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Predicting Function from Structure: 3D Structure Studies of the Mammalian Golgi Complex by Mogelsvang, Soren, Marsh, Brad J., Ladinsky, Mark S., Howell, Kathryn E.

“…3D electron tomography studies of the structure of the mammalian Golgi complex have led to four functional predictions (1). The sorting and exit site from the…”
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Efficient automatic noise reduction of electron tomographic reconstructions based on iterative median filtering by van der Heide, Peter, Xu, Xiao-Ping, Marsh, Brad J., Hanein, Dorit, Volkmann, Niels

“…A simple, fast and efficient noise-reduction protocol for three-dimensional electron tomographic reconstructions of biological material is presented. The…”
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A 3D image filter for parameter-free segmentation of macromolecular structures from electron tomograms by Ali, Rubbiya A, Landsberg, Michael J, Knauth, Emily, Morgan, Garry P, Marsh, Brad J, Hankamer, Ben

“…3D image reconstruction of large cellular volumes by electron tomography (ET) at high (≤ 5 nm) resolution can now routinely resolve organellar and…”
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Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography by Marsh, Brad J.

“…It is now possible to dissect subcellular membrane architecture and dynamics at the nanoanatomical level in 3D through the development of a technique known as…”
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Illoura™: a software tool for analysis, visualization and semantic querying of cellular and other spatial biological data by McComb, Tim, Cairncross, Oliver, Noske, Andrew B., Wood, David L. A., Marsh, Brad J., Ragan, Mark A.

“…New high-resolution approaches for mapping ultrastructure of cells in 3D are leading to unprecedented quantities of spatial data. Here we present Illoura, a…”
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β-cell ABCA1 influences insulin secretion, glucose homeostasis and response to thiazolidinedione treatment by Verchere, C Bruce, Hayden, Michael R, Brunham, Liam R, Kruit, Janine K, Pape, Terry D, Timmins, Jenelle M, Reuwer, Anne Q, Vasanji, Zainisha, Marsh, Brad J, Rodrigues, Brian, Johnson, James D, Parks, John S

“…Type 2 diabetes is characterized by both peripheral insulin resistance and reduced insulin secretion by beta-cells. The reasons for beta-cell dysfunction in…”
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Regulated Autophagy Controls Hormone Content in Secretory-Deficient Pancreatic Endocrine β-Cells by Marsh, Brad J, Soden, Chad, Alarcón, Cristina, Wicksteed, Barton L, Yaekura, Kazuro, Costin, Adam J, Morgan, Garry P, Rhodes, Christopher J

“…Endocrine cells are continually regulating the balance between hormone biosynthesis, secretion, and intracellular degradation to ensure that cellular hormone…”
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Viewing Golgi Structure and Function from a Different Perspective—Insights from Electron Tomography by Marsh, Brad J., Pavelka, Margit

“…Historically, ultrastructural investigations, which have focused on elucidating the biological idiosyncrasies of the Golgi apparatus, have tended towards…”
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The mammalian Golgi - complex debates by Marsh, Brad J, Howell, Kathryn E

“…Since the first description of the Golgi in 1898, key issues regarding this organelle have remained contentious among cell biologists. Resolving these complex…”
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Effects of insulin on intracellular GLUT4 vesicles in adipocytes: evidence for a secretory mode of regulation by Martin, S, Millar, C A, Lyttle, C T, Meerloo, T, Marsh, B J, Gould, G W, James, D E

“…The facilitative glucose transporter, GLUT4 undergoes insulin-dependent movement to the cell surface in adipocytes. The magnitude of the insulin effect is much…”
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