Search Results - "Markoulatos, P."

Molecular approaches for HPV genotyping and HPV-DNA physical status by Tsakogiannis, D, Gartzonika, C, Levidiotou-Stefanou, S, Markoulatos, P

“…Persistent infection with high-risk human papillomavirus (HPV) genotypes is the leading cause of cervical cancer development. To this end several studies have…”
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Genetic variability of the HPV16 early genes and LCR. Present and future perspectives by Bletsa, G, Zagouri, F, Amoutzias, G D, Nikolaidis, M, Zografos, E, Markoulatos, P, Tsakogiannis, D

“…Human papillomavirus 16 (HPV16) infection is the aetiologic factor for the development of cervical dysplasia and is regarded as highly carcinogen, because it…”
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WarmStart colorimetric LAMP for the specific and rapid detection of HPV16 and HPV18 DNA by Daskou, M., Tsakogiannis, D., Dimitriou, T.G., Amoutzias, G.D., Mossialos, D., Kottaridi, C., Gartzonika, C., Markoulatos, P.

“…•The WarmStart colorimetric LAMP was developed for the detection of HPV16, HPV18.•The WarmStart colorimetric LAMP amplify the E6 gene of HPV16 and HPV18…”
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WarmStart colorimetric RT‐LAMP for the rapid, sensitive and specific detection of Enteroviruses A–D targeting the 5′UTR region by Daskou, M., Dimitriou, T.G., Alexopoulou, D.S., Tsakogiannis, D., Amoutzias, G.D., Mossialos, D., Kyriakopoulou, Z., Markoulatos, P.

“…Aims The aim of the present study was to develop a colorimetric LAMP assay for the detection of enteroviruses belonging to species A–D targeting the 5′…”
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Multiplex polymerase chain reaction: A practical approach by Markoulatos, P., Siafakas, N., Moncany, M.

“…Considerable time and effort can be saved by simultaneously amplifying multiple sequences in a single reaction, a process referred to as multiplex polymerase…”
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A colorimetric IsoPCR for the rapid and sensitive visual detection of high-risk HPV16 in clinical samples with hydroxynaphthol blue by Daskou, M., Tsakogiannis, D., Alexopoulou, D.S., Dimitriou, T.G, Mossialos, D., Amoutzias, G.D, Kottaridi, C., Markoulatos, P.

“…•A simple and fast methodology was developed for visual detection of HPV16 DNA.•The method merges the benefits of PCR, LAMP and the assets of HNB…”
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Polymorphic variability in the exon 19 of the RB1 gene and its flanking intronic sequences in HPV16-associated precancerous lesions in the Greek population by Tsakogiannis, D, Moschonas, G D, Daskou, M, Stylianidou, Z, Kyriakopoulou, Z, Kottaridi, C, Dimitriou, T G, Gartzonika, C, Markoulatos, P

“…The tumour suppressor protein RB plays a decisive role in negative control of the cell cycle, inhibiting tumour development. The present analysis investigated…”
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Sites of disruption within E1 and E2 genes of HPV16 and association with cervical dysplasia by Tsakogiannis, D., Gortsilas, P., Kyriakopoulou, Z., Ruether, I.G.A., Dimitriou, T.G., Orfanoudakis, G., Markoulatos, P.

“…Integration of HPV16 DNA into the host chromosome usually disrupts the E1 and/or E2 genes. The present study investigated the disruption of E1, E2 genes in a…”
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Development of a reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) that detects enteroviruses by targeting the highly conserved 5′-UTR region by Daskou, M., Dimitriou, T. G., Kouklamani-Giannouli, G., Nikolaidis, M., Mossialos, D., Amoutzias, G. D., Markoulatos, P.

“…Enteroviruses are positive sense single-stranded RNA viruses. Most infections from enteroviruses are asymptomatic and can circulate “silently”, increasing the…”
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Α 2-stage, nested-like nucleic acid amplification method (IsoPCR) for the highly sensitive detection of HPV16 and HPV18 DNA by Daskou, M., Tsakogiannis, D., Dimitriou, T.G., Manali, M., Apti, C., Amoutzias, G.D., Mossialos, D., Kottaridi, C., Markoulatos, P.

“…Molecular detection of HPV DNA is considered as the gold standard for the diagnosis of cervical disease. Although the molecular assays for the identification…”
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Detection of negative and positive RNA strand of poliovirus Sabin 1 and echovirus E19 by a stem‐loop reverse transcription PCR by Fikatas, A., Dimitriou, T.G., Kyriakopoulou, Z., Moschonas, G.D., Amoutzias, G.D., Mossialos, D., Gartzonika, C., Levidiotou‐Stefanou, S., Markoulatos, P.

“…In this report a strand specific RT‐PCR was established for the detection of the replicative negative RNA strand of poliovirus sabin 1 (Sabin1) and Echovirus…”
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Identification of rearranged sequences of HPV16 DNA in precancerous and cervical cancer cases by Tsakogiannis, D., Bletsa, M., Kyriakopoulou, Z., Dimitriou, T.G., Kotsovassilis, C., Panotopoulou, E., Markoulatos, P.

“…Integration of HPV16 DNA into the host chromosome is considered to be a crucial step towards genomic instability and cervical cancer development. Aim of the…”
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Multiplex PCR assay for the rapid identification of human papillomavirus genotypes 16, 18, 45, 35, 66, 33, 51, 58, and 31 in clinical samples by Tsakogiannis, D, Diamantidou, V, Toska, E, Kyriakopoulou, Z, Dimitriou, T. G, Ruether, I. G. A, Gortsilas, P, Markoulatos, P

“…The causal association between persistent human papillomavirus (HPV) infection and cervical cancer has lead to the development of a variety of molecular assays…”
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Molecular and evolutionary analysis of HPV16 E6 and E7 genes in Greek women by TSAKOGIANNIS, D, PAPADOPOULOU, A, KONTOSTATHI, G, RUETHER, I. G. A, KYRIAKOPOULOU, Z, DIMITRIOU, T. G, ORFANOUDAKIS, G, MARKOULATOS, P

“…Human papillomavirus type 16 (HPV16) non-European variants have been associated with persistent infection and cervical cancer development, while the L83V…”
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Nucleotide polymorphisms of the human papillomavirus 16 E1 gene by Tsakogiannis, D, Darmis, F, Gortsilas, P, Ruether, I. G. A, Kyriakopoulou, Z, Dimitriou, T. G, Amoutzias, G, Markoulatos, P

“…The E1 ORF is one of the most conserved regions in the human papillomavirus (HPV) genome. The complete E1 gene of the HPV16 genome was amplified with four…”
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Development of a multiplex RT-PCR assay for the identification of recombination types at different genomic regions of vaccine-derived polioviruses by Dimitriou, T. G., Kyriakopoulou, Z., Tsakogiannis, D., Fikatas, A., Gartzonika, C., Levidiotou-Stefanou, S., Markoulatos, P.

“…Polioviruses (PVs) are the causal agents of acute paralytic poliomyelitis. Since the 1960s, poliomyelitis has been effectively controlled by the use of two…”
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Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66 by Tsakogiannis, D., Papacharalampous, M., Toska, E., Kyriakopoulou, Z., Dimitriou, T.G., Ruether, I.G.A., Komiotis, D., Markoulatos, P.

“…Long-term infection with high-risk HPV genotypes is the leading cause of cervical cancer. In the present study a Duplex Real-time PCR assay was developed in…”
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Complete nucleotide sequence analysis of the VP1 genomic region of Echoviruses 6 isolated from sewage in Greece revealed 98% similarity with Echoviruses 6 that were characterized from an aseptic meningitis outbreak 1year later by Kyriakopoulou, Z, Dedepsidis, E, Pliaka, V, Tsakogiannis, D, Ruether, IGA, Krikelis, A, Markoulatos, P

“…The molecular characterization of two enterovirus strains (LR51A5 and LR61G3) isolated from the sewage treatment plant unit in Larissa, Greece, in May and June…”
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Complete nucleotide sequence analysis of the VP1 genomic region of Echoviruses 6 isolated from sewage in Greece revealed 98% similarity with Echoviruses 6 that were characterized from an aseptic meningitis outbreak 1 year later by Kyriakopoulou, Z., Dedepsidis, E., Pliaka, V., Tsakogiannis, D., Ruether, I.G.A., Krikelis, A., Markoulatos, P.

“…The molecular characterization of two enterovirus strains (LR51A5 and LR61G3) isolated from the sewage treatment plant unit in Larissa, Greece, in May and June…”
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Sequence variation analysis of the E2 gene of human papilloma virus type 16 in cervical lesions from women in Greece by Tsakogiannis, D., Ruether, I. G. A., Kyriakopoulou, Z., Pliaka, V., Theoharopoulou, A., Skordas, V., Panotopoulou, E., Nepka, C., Markoulatos, P.

“…The E2 gene of human papilloma virus is expressed at the early stage of the viral life cycle, encoding the E2 transcription factor, and regulates the…”
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