Search Results - "Mark E. Dumont"

Comparison of Experimental Approaches Used to Determine the Structure and Function of the Class D G Protein-Coupled Yeast α-Factor Receptor by Dumont, Mark E., Konopka, James B.

“…The Saccharomyces cerevisiae α-factor mating pheromone receptor (Ste2p) has been studied as a model for the large medically important family of G…”
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Structure of the SLC4 transporter Bor1p in an inward‐facing conformation by Coudray, Nicolas, L. Seyler, Sean, Lasala, Ralph, Zhang, Zhening, Clark, Kathy M., Dumont, Mark E., Rohou, Alexis, Beckstein, Oliver, Stokes, David L.

“…Bor1p is a secondary transporter in yeast that is responsible for boron transport. Bor1p belongs to the SLC4 family which controls bicarbonate exchange and pH…”
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Structure of the Integral Membrane Protein CAAX Protease Ste24p by Pryor, Edward E., Horanyi, Peter S., Clark, Kathleen M., Fedoriw, Nadia, Connelly, Sara M., Koszelak-Rosenblum, Mary, Zhu, Guangyu, Malkowski, Michael G., Wiener, Michael C., Dumont, Mark E.

“…Posttranslational lipidation provides critical modulation of the functions of some proteins. Isoprenoids (i.e., farnesyl or geranylgeranyl groups) are attached…”
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Identification of variant HIV envelope proteins with enhanced affinities for precursors to anti-gp41 broadly neutralizing antibodies by Zhu, Hong, Mathew, Elizabeth, Connelly, Sara M, Zuber, Jeffrey, Sullivan, Mark, Piepenbrink, Michael S, Kobie, James J, Dumont, Mark E

“…HIV envelope protein (Env) is the sole target of broadly neutralizing antibodies (BNAbs) that are capable of neutralizing diverse strains of HIV. While BNAbs…”
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Display of the HIV envelope protein at the yeast cell surface for immunogen development by Mathew, Elizabeth, Zhu, Hong, Connelly, Sara M, Sullivan, Mark A, Brewer, Matthew G, Piepenbrink, Michael S, Kobie, James J, Dewhurst, Stephen, Dumont, Mark E

“…As a step toward the development of variant forms of Env with enhanced immunogenic properties, we have expressed the glycoprotein in the yeast surface display…”
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Functional and Physical Interactions among Saccharomyces cerevisiae α-Factor Receptors by Gehret, Austin U, Connelly, Sara M, Dumont, Mark E

“…Classifications Services EC Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Oligomerization of yeast α-factor receptor detected by fluorescent energy transfer between ligands by Connelly, Sara M., Sridharan, Rajashri, Naider, Fred, Dumont, Mark E.

“…G-protein-coupled receptors (GPCRs) comprise a large superfamily of transmembrane receptors responsible for transducing responses to the binding of a wide…”
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Fluorescent approaches for understanding interactions of ligands with G protein coupled receptors by Sridharan, Rajashri, Zuber, Jeffrey, Connelly, Sara M., Mathew, Elizabeth, Dumont, Mark E.

“…G protein coupled receptors are responsible for a wide variety of signaling responses in diverse cell types. Despite major advances in the determination of…”
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Expression of 25 Human ABC Transporters in the Yeast Pichia pastoris and Characterization of the Purified ABCC3 ATPase Activity by Chloupková, Maja, Pickert, Amanda, Lee, Jyh-Yeuan, Souza, Shiloe, Trinh, Yenphuong T, Connelly, Sara M, Dumont, Mark E, Dean, Michael, Urbatsch, Ina L

“…Human ATP-binding cassette (ABC) transporters comprise a family of 48 membrane-spanning transport proteins, many of which are associated with genetic diseases…”
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A Facile Method for High-throughput Co-expression of Protein Pairs by Alexandrov, Andrei, Vignali, Marissa, LaCount, Douglas J, Quartley, Erin, de Vries, Christina, De Rosa, Daniela, Babulski, Julie, Mitchell, Sarah F, Schoenfeld, Lori W, Fields, Stanley, Hol, Wim G, Dumont, Mark E, Phizicky, Eric M, Grayhack, Elizabeth J

“…We developed a method to co-express protein pairs from collections of otherwise identical Escherichia coli plasmids expressing different ORFs by incorporating…”
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Characteristics Affecting Expression and Solubilization of Yeast Membrane Proteins by White, Michael A., Clark, Kathleen M., Grayhack, Elizabeth J., Dumont, Mark E.

“…Biochemical and structural analysis of membrane proteins often critically depends on the ability to overexpress and solubilize them. To identify properties of…”
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The Crystal Structure of an Integral Membrane Fatty Acid α-Hydroxylase by Zhu, Guangyu, Koszelak-Rosenblum, Mary, Connelly, Sara M., Dumont, Mark E., Malkowski, Michael G.

“…Neuronal electrical impulse propagation is facilitated by the myelin sheath, a compact membrane surrounding the axon. The myelin sheath is highly enriched in…”
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Purification and ATP Hydrolysis of the Putative Cholesterol Transporters ABCG5 and ABCG8 by Wang, Zhanling, Stalcup, Lindsay D, Harvey, Brandy J, Weber, Joachim, Chloupkova, Maja, Dumont, Mark E, Dean, Michael, Urbatsch, Ina L

“…Mutations in the ATP-binding cassette (ABC) transporters ABCG5 and ABCG8 lead to sitosterolemia, a disorder characterized by sterol accumulation and premature…”
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Human CaaX protease ZMPSTE24 expressed in yeast: Structure and inhibition by HIV protease inhibitors by Clark, Kathleen M., Jenkins, Jermaine L., Fedoriw, Nadia, Dumont, Mark E.

“…The function and localization of proteins and peptides containing C‐terminal “CaaX” (Cys‐aliphatic‐aliphatic‐anything) sequence motifs are modulated by…”
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Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein-coupled Receptor in Yeast by Sridharan, Rajashri, Connelly, Sara M., Naider, Fred, Dumont, Mark E.

“…We report here on the relationship between ligand binding and signaling responses in the yeast pheromone response pathway, a well characterized G…”
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A Novel Screening Approach for Optimal and Functional Fusion of T4 Lysozyme in GPCRs by Mathew, Elizabeth, Dumont, Mark E

“…Determination of high-resolution, three-dimensional structures of transmembrane proteins (TMPs) has, in many cases, only been accomplished through the use of…”
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Biochemical and genetic analysis of the yeast proteome with a movable ORF collection by Gelperin, Daniel M, White, Michael A, Wilkinson, Martha L, Kon, Yoshiko, Kung, Li A, Wise, Kevin J, Lopez-Hoyo, Nelson, Jiang, Lixia, Piccirillo, Stacy, Yu, Haiyuan, Gerstein, Mark, Dumont, Mark E, Phizicky, Eric M, Snyder, Michael, Grayhack, Elizabeth J

“…Functional analysis of the proteome is an essential part of genomic research. To facilitate different proteomic approaches, a MORF (moveable ORF) library of…”
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Identification of Destabilizing and Stabilizing Mutations of Ste2p, a G Protein-Coupled Receptor in Saccharomyces cerevisiae by Zuber, Jeffrey, Danial, Shairy Azmy, Connelly, Sara M, Naider, Fred, Dumont, Mark E

“…The isolation of mutations affecting the stabilities of transmembrane proteins is useful for enhancing the suitability of proteins for structural…”
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Deducing the symmetry of helical assemblies: Applications to membrane proteins by Coudray, Nicolas, Lasala, Ralph, Zhang, Zhening, Clark, Kathy M., Dumont, Mark E., Stokes, David L.

“…Helical reconstruction represents a convenient and powerful approach for structure determination of macromolecules that assemble into helical arrays. In the…”
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Coordinating the impact of structural genomics on the human α-helical transmembrane proteome by Pieper, Ursula, Schlessinger, Avner, Kloppmann, Edda, Chang, Geoffrey A, Chou, James J, Dumont, Mark E, Fox, Brian G, Fromme, Petra, Hendrickson, Wayne A, Malkowski, Michael G, Rees, Douglas C, Stokes, David L, Stowell, Michael H B, Wiener, Michael C, Rost, Burkhard, Stroud, Robert M, Stevens, Raymond C, Sali, Andrej

“…Given the recent successes in determining membrane-protein structures, we explore the tractability of determining representatives for the entire human membrane…”
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